In protists a biodiversity hotspot could be defined as region with exceptionally high ratio of local to global species diversity. In 2012 Aquitaine (France) was revealed to represent a hotspot of Synurales (lineage within silica-scaled chrysophytes). To investigate how the diversity is distributed and where the species are hidden, samples from 42 lakes were collected during spring peak of Chrysophyte occurrence (from March 27th to March 31st ). Samples were taken in two microbiotopes - phytoplankton and metaphyton. Metaphyton was represented by different substrates (aquatic plants). I identified seventy-six species from genera Mallomonas and Synura, which confirmed Aquitaine to be a hotspot. In this thesis, I investigated ecological drivers of chrysophytes diversity and community structure. The chrysophyte diversity was driven mostly by mutual impact of pH and conductivity, north-south gradient of lakes location and sampled microbiotopes. Number of identified species increased with increasing pH and conductivity, with increasing size of waterbody and with location of the lake more towards the South. More taxa were revealed from metaphyton (77) than from plankton (61), substrates played an important role. Lakes in Aquitaine are rich in different species of aquatic plant, which immersed create...