Abstract A new technique for bonding of polymer micro-fluidic devices has been developed. This method can easily bond biochips with complex flow patterns and metal layer. Above all, using a patterned glass, the micro-channel structures on Poly-Methyl Meth-Acrylate (PMMA) substrates were generated by one-step hot embossing procedure. In contrast with the traditional thermal bonding, this paper presents low-temperature and low-pressure packaging for polymer micro-fluidic platforms. Furthermore, the disposable plastic biochip has successfully been tested by the measurement of tensile strength and surface roughness. This paper also reports details of the passive and active micro-mixers. According to experimental and numerical investigations, the mixing performance of passive micro-mixers is expectably to be found. In addition, to quantify the mixing concentration distribution in the micro-channel, it has been demonstrated by launching the image analysis programs. The bonding efficiency of the solvent is twenty four times as strong as thermal bonding efficiency.