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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

The use of microsatellites as a surrogate for quantitative trait variation in conservation

Gunn, Melissa Rose, School of Biological, Earth & Environmental Science, UNSW January 2003 (has links)
Conservation biologists are interested in maintaining genetic variation in small populations, with a view to maintaining fitness and the ability of the species to adapt to changing environmental conditions. The most important type of genetic variation is therefore that which affects fitness and reproduction, and is therefore subject to natural selection. Such fitness traits are often quantitative, i.e. are the result of a suite of loci, and are continuously variable. Microsatellite markers are a popular method of determining the level of variation present in a species??? genome. The assumption is made that microsatellites, which are neutral markers, behave in the same manner as quantitative traits. If this assumption were proved incorrect, then the use of neutral markers in conservation monitoring would have to be re-evaluated. In this study, experiments have been conducted using Drosophila melanogaster to test the assumption that variation in quantitative traits under stabilising selection declines at the same rate as heterozygosity in microsatellite markers, during a population bottleneck. Experimental population bottlenecks were of two effective population sizes (Ne), Ne=2 for one generation and Ne=60 for 35 generations. Based on the effective population size, we expected both types of bottlenecks to lose 25% of neutral genetic variation. Ten replicates of each bottleneck were maintained, along with four large control populations with Ne=320. In each population, heterozygosity (He) for eight microsatellite loci was compared with the heritability and additive genetic variance of two quantitative traits subject to balancing selection: fecundity and sternopleural bristle number. Microsatellite heterozygosity decreased in accordance with neutral predictions, whereas additive genetic variation in quantitative traits altered more than expected in both large and in bottlenecked populations relative to the initial sampling values, indicating that variation in quantitative traits was not being lost at the same rate as predicted by neutral theory. For most traits, the changes in additive genetic variance were congruent in all populations, large or bottlenecked. This congruence suggests that a common process was affecting all populations, such as adaptation. A mite infestation in early generations is a possible source of selective pressure. When bottlenecked populations were compared to the contemporaneous large populations (Ne = 320), the additive genetic variance of most traits was seen to have been lost in accordance with predictions from the loss of microsatellite heterozygosity. Loss of variation in microsatellites can thus be used to predict the loss of variation in quantitative traits due to bottlenecks, but not to predict the potentially much larger changes due to other processes such as adaptation. The effects of concurrent environmental stress and reduced population size were also evaluated. Endangered populations are often subject to environmental stress in addition to reduced population size, but the effect of stress on the additive genetic variance of fitness traits in organisms undergoing population bottlenecks is unknown. If the presence of stress alters the level of additive genetic variance in fitness traits, the viability of such populations could be substantially affected. The loss of microsatellite heterozygosity was not affected by the presence of a stress agent during a bottleneck. I found some significant effects of stress on the additive genetic variance of sternopleural bristles and fecundity; there was also a significant interaction between stress and the response to directional selection in sternopleural bristles. There was also an increase in the coefficient of variation of VA for sternopleural bristles. Stress may therefore affect the manner in which populations respond to selective pressures.
2

Applications and Orbit Scenarios for a Multistatic InSAR Formation Flying Microsatellite Mission

Peterson, Erica H. 26 February 2009 (has links)
The Space Flight Laboratory (SFL) at the University of Toronto Institute for Aerospace Studies is currently designing CanX-4 and CanX-5, a pair of formation-flying nanosatellites that will target centimeter-level position determination and sub-meter control. Once formation flight has been demonstrated, future missions can carry payloads designed to exploit these capabilities. Earth Observation is one such application that can benefit greatly from the availability of multiple platforms with precise position determination and attitude control. This work explores multistatic interferometric synthetic aperture radar (InSAR) as a particularly promising implementation of formation flight. Several mission scenarios are considered, including three commonly proposed InSAR constellation configurations, namely the Cartwheel, the Cross-Track Pendulum, and the Car-Pe configuration, as well as three large ( kilowatt) SAR transmitters (L-, C- and X-band) and one microsatellite transmitter (X-band, 150W). Using a framework of STK and MATLAB simulation and analysis tools, each case is evaluated with respect to the available interferometric baselines, ground coverage, resolution, and utility for selected applications including digital elevation modeling, moving target detection, and superresolution imagery. The “large” X-band transmitter is found to produce the most favorable operating area and resolution, and the Car-Pe configuration provides the greatest utility and flexibility for a combination of the three selected applications.
3

Applications and Orbit Scenarios for a Multistatic InSAR Formation Flying Microsatellite Mission

Peterson, Erica H. 26 February 2009 (has links)
The Space Flight Laboratory (SFL) at the University of Toronto Institute for Aerospace Studies is currently designing CanX-4 and CanX-5, a pair of formation-flying nanosatellites that will target centimeter-level position determination and sub-meter control. Once formation flight has been demonstrated, future missions can carry payloads designed to exploit these capabilities. Earth Observation is one such application that can benefit greatly from the availability of multiple platforms with precise position determination and attitude control. This work explores multistatic interferometric synthetic aperture radar (InSAR) as a particularly promising implementation of formation flight. Several mission scenarios are considered, including three commonly proposed InSAR constellation configurations, namely the Cartwheel, the Cross-Track Pendulum, and the Car-Pe configuration, as well as three large ( kilowatt) SAR transmitters (L-, C- and X-band) and one microsatellite transmitter (X-band, 150W). Using a framework of STK and MATLAB simulation and analysis tools, each case is evaluated with respect to the available interferometric baselines, ground coverage, resolution, and utility for selected applications including digital elevation modeling, moving target detection, and superresolution imagery. The “large” X-band transmitter is found to produce the most favorable operating area and resolution, and the Car-Pe configuration provides the greatest utility and flexibility for a combination of the three selected applications.
4

Genomic analysis of the horse Y chromosome

Santani, Avni Bhawan 17 February 2005 (has links)
Stallion fertility is of significant economic importance in the multibillion dollar equine industry. Presently, the underlying genetic causes of infertility in stallions are unknown. Analysis of the human genome has shown that in more than 25% of cases, male infertility is associated with deletions/rearrangements in the Y chromosome. Presently there is no gene map for the Y chromosome in the horse. Therefore, the primary aim of this study is to build a detailed physical map of the chromosome with a long-term aim to identify and analyze Y-specific factors affecting fertility in stallions. To materialize this, we constructed the first radiation hybrid and FISH map of the euchromatic region of the horse Y chromosome. This basic map was used to obtain Y-specific BAC clones that provided new STS markers from the end sequences. Chromosome walking provided 73 BACs comprising 7 contigs that were built across the euchromatic region using 124 markers for content mapping. The results were validated by restriction fingerprinting and Fiber FISH. The map is presently the most informative among the domestic species and second to only human and mouse Y chromosome maps. The construction of this map will pave the way for isolation and functional characterization of genes critical for normal male fertility and reproduction and will in the future lead to the development of a diagnostic test to facilitate early identification of deletions/rearrangements on the Y chromosome of potentially affected foals/stallions. The second part of the study comprised the first extended investigation to assess genetic variation in the horse Y chromosome. Approximately 4.5Mb of the euchromatic region was screened for polymorphic microsatellite markers. Of the 27 markers that were characterized and screened for polymorphism in 14 breeds of the domestic horse and eight extant equids, only one was polymorphic in the domestic horse, suggesting a low level of genetic variation on the chromosome. However, 21 of the markers showed noteworthy variation (on average four alleles/marker) among the eight equids. These markers will be vital in future studies aimed at elucidating the genetic relationships between the various equids through phylogenetic analysis.
5

Genotyping of gestational trophoblastic disease

Lai, Yau-lin, Caroline. January 2001 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 49-58).
6

Variation among Phytophthora cinnamomi isolates from oak forest soils in the eastern United States

Eggers, Jordan. January 1900 (has links)
Thesis (M.S.)--West Virginia University, 2009. / Title from document title page. Document formatted into pages; contains viii, 56 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 52-56).
7

The use of microsatellites as a surrogate for quantitative trait variation in conservation

Gunn, Melissa Rose, School of Biological, Earth & Environmental Science, UNSW January 2003 (has links)
Conservation biologists are interested in maintaining genetic variation in small populations, with a view to maintaining fitness and the ability of the species to adapt to changing environmental conditions. The most important type of genetic variation is therefore that which affects fitness and reproduction, and is therefore subject to natural selection. Such fitness traits are often quantitative, i.e. are the result of a suite of loci, and are continuously variable. Microsatellite markers are a popular method of determining the level of variation present in a species??? genome. The assumption is made that microsatellites, which are neutral markers, behave in the same manner as quantitative traits. If this assumption were proved incorrect, then the use of neutral markers in conservation monitoring would have to be re-evaluated. In this study, experiments have been conducted using Drosophila melanogaster to test the assumption that variation in quantitative traits under stabilising selection declines at the same rate as heterozygosity in microsatellite markers, during a population bottleneck. Experimental population bottlenecks were of two effective population sizes (Ne), Ne=2 for one generation and Ne=60 for 35 generations. Based on the effective population size, we expected both types of bottlenecks to lose 25% of neutral genetic variation. Ten replicates of each bottleneck were maintained, along with four large control populations with Ne=320. In each population, heterozygosity (He) for eight microsatellite loci was compared with the heritability and additive genetic variance of two quantitative traits subject to balancing selection: fecundity and sternopleural bristle number. Microsatellite heterozygosity decreased in accordance with neutral predictions, whereas additive genetic variation in quantitative traits altered more than expected in both large and in bottlenecked populations relative to the initial sampling values, indicating that variation in quantitative traits was not being lost at the same rate as predicted by neutral theory. For most traits, the changes in additive genetic variance were congruent in all populations, large or bottlenecked. This congruence suggests that a common process was affecting all populations, such as adaptation. A mite infestation in early generations is a possible source of selective pressure. When bottlenecked populations were compared to the contemporaneous large populations (Ne = 320), the additive genetic variance of most traits was seen to have been lost in accordance with predictions from the loss of microsatellite heterozygosity. Loss of variation in microsatellites can thus be used to predict the loss of variation in quantitative traits due to bottlenecks, but not to predict the potentially much larger changes due to other processes such as adaptation. The effects of concurrent environmental stress and reduced population size were also evaluated. Endangered populations are often subject to environmental stress in addition to reduced population size, but the effect of stress on the additive genetic variance of fitness traits in organisms undergoing population bottlenecks is unknown. If the presence of stress alters the level of additive genetic variance in fitness traits, the viability of such populations could be substantially affected. The loss of microsatellite heterozygosity was not affected by the presence of a stress agent during a bottleneck. I found some significant effects of stress on the additive genetic variance of sternopleural bristles and fecundity; there was also a significant interaction between stress and the response to directional selection in sternopleural bristles. There was also an increase in the coefficient of variation of VA for sternopleural bristles. Stress may therefore affect the manner in which populations respond to selective pressures.
8

Genetic variability and population differentiation in Scandinavian wolverines

Duffy, Andrew J. January 1997 (has links) (PDF)
Thesis (M.S.)--Dalhousie University, 1998. / Includes bibliographical references (leaves 65-73).
9

Genetic differentiation in Alewife populations using microsatellite loci

Chilakamarri, Sunita R. January 2005 (has links)
Thesis (M.S.) -- Worcester Polytechnic Institute. / Keywords: fixation indices; genetic differentiation; microsatellite; alewife. Includes bibliographical references (p.43-51).
10

Genetic analysis of rainbow trout (Oncorhynchus mykiss) strain identification via microsatellites and analysis of expressed sequence tags in intestine, liver, kidney, and ovary /

Stewart, Amanda B. January 1900 (has links)
Thesis (Ph. D.)--West Virginia University, 2006. / Title from document title page. Document formatted into pages; contains vi, 153 p. : ill. Includes abstract. Includes bibliographical references (p. 53-100).

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