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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Ação antifúngica do óleo essencial de Rosa alba L. / Antifungal action of essential oil of Rosa alba L.

Reis, Oswaldo Henrique Barolli 23 February 2011 (has links)
Made available in DSpace on 2016-05-02T13:55:30Z (GMT). No. of bitstreams: 1 OswaldoHenriqueBarolliReis-Dissertacao.pdf: 707277 bytes, checksum: 3b71c133dbad0b57929d4b255619719c (MD5) Previous issue date: 2011-02-23 / In swine culture, the study of mycology contributes to food security in the understanding of various diseases. A knowledge of fungal agents on the skin of healthy animals and surface pathologies is important for effective control of the potential risks of infection, especially when associated with confined species, where the imbalance in the microbiota of the environment can be crucial in the interactions between environment and animals. The objective was to investigate the in vitro antimicrobial activity of essential oil of Rosa alba L. petals on microbial growth of yeasts Candida albicans (ATCC 10231) and filamentous fungi that causes opportunistic infectious processes in the skin of pigs, such as Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) and Trichophyton tonsurans (ATCC 10870). The oils were extracted by the process of hydrodistillation in Clevenger apparatus, using fresh white rose petals. Microbiological tests were performed by the agar diffusion method (pour-plate) with Sabouraud-dextrose. Wells 5 mm in diameter, produced in the agar, were filled with 15 μL of essential oil and the plates containing filamentous fungi were incubated in an inverted position at 28 °C for 10 days and the plates containing yeast were incubated at 30 °C for 48 hours. After this time, the inhibition circles were measured. The data were subjected to analysis of variance and means were compared by Tukey`s test at 5% probability. It was found that the activity of essential oil under study is significant in relation to the growth of microbial species tested. / Na suinocultura, o estudo da micologia contribui tanto na segurança alimentar como no entendimento de diversas patologias. O conhecimento dos agentes fúngicos presentes na pele de animais saudáveis e com patologias superficiais é importante para um controle eficaz dos riscos potenciais de infecção, principalmente quando associados a espécies confinadas, onde o desequilíbrio da microbiota ambiental pode ser decisivo nas interações entre o ambiente e os animais. Objetivou-se investigar a atividade antimicrobiana in vitro do óleo essencial de pétalas de Rosa alba L. sobre o crescimento microbiano de leveduras Candida albicans (ATCC 10231) e fungos filamentosos causadores de processos infecciosos oportunistas na pele de suínos, tais como Microsporum nanum (ATCC 11832), Trichophyton mentagrophytes (ATCC 9533), Trichophyton rubrum (ATCC 10218) e Trichophyton tonsurans (ATCC 10870). Os óleos foram extraídos pelo processo de hidrodestilação em aparelho de Clevenger, utilizando partes frescas das pétalas de rosa branca. Os testes microbiológicos foram realizados pelo método de difusão em ágar ( pour-plate ) com meio de cultura ágar Sabouraud-dextrose. Poços de 5 mm de diâmetro, produzidos no ágar, foram preenchidos com 15 μL do óleo essencial e, em seguida, as placas contendo os fungos filamentosos foram incubadas de forma invertida, a 28 ºC por 10 dias; e as placas contendo a levedura foram incubadas a 30 ºC por 48 horas. Passado este tempo, os halos de inibição tiveram seus diâmetros medidos. Os dados obtidos foram submetidos à análise de variância e as médias comparadas através do teste de Tukey ao nível de 5% de probabilidade. Verificou-se que a atividade do óleo essencial em estudo é significativa em relação ao crescimento das espécies microbianas testadas.

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