The aim of my thesis was to analyze the quality parameters of raw bulk milk with a focus on hygienic and microbiological quality. For this purpose we choose two markers: somatic cell count (SCC) and total bacterial count (TBC). Data were obtained from two dairy farms Dolní Bukovsko and Popovice for the period from June 2008 to February 2011. For statistical evaluation was used MS Excel and Statistics 6.0. SCC and the average TBC values were recorded at higher density in Dolní Bukovsko. However, the averages for these two breeds are consistent with the legislation of the European Union and the Czech Republic (both TBC (?100,000/ml) and SCC (?400,000/ml)). Even though the average TBC value exceeded the limit of the breeding herd health PSB ? 200 thousand / ml, indicating the possible presence of subclinical mastitis and bonded with the considerable economic losses in milk production.

Forest ecosystems represent a huge reservoir of carbon. The volume of deadwood in managed forests is in the tens of m3 h-1, while unmanaged forests have the volume of deadwood in hundreds of m3 ha- 1. Deadwood is a substrate with a high potential for decomposition. However, deadwood is a specific habitat that has a high C/N ratio and changes due to ongoing decomposition. The C/N ratio decreases with increasing decay time. In addition to the C/N ratio, the pH also changes during the wood pulping process, due to the decomposition of plant biomass by fungi. Moreover, deadwood has a high impermeability. The main decomposers of dead wood are fungi. In addition to them, bacteria, which are numerous in deadwood, also contribute to decomposition. Bacterial communities that decompose woody biomass are affected by these factors, and their distribution is also affected by dead tree species. The aim of this study was to describe how bacterial communities associated with deadwood change during decay. The most significant factor in the decomposition by bacterial communities is the time of decay. Bacteria found in short-decaying wood (less than 16 years old) are more diverse than those found in long-decaying wood. Methylotrophic bacteria and N-fixing bacteria are common in short-decaying wood as well. N-fixing...

In my thesis, I focused on monitoring of microorganisms in the sample of red grape juice and on the interactions between yeasts, bacteria and filamentous fungi. Three different media were applied for the cultivation of microorganisms; firstly for monitoring of total volume of microorganisms, secondly for yeasts and third time for lactic acid bacteria. The indirect method was used for the determination of the amount of viable cells. This method consists in enumerating of visible macroscopic colonies grown up on agar plates. When the cells grew up, the forms of colonies were analyzed visually and the morphology of microorganisms was detected microscopically. The operating time of enzymes in grape juice in the production of red wine was monitored after application of commercial enzymatic preparation. The enzym action in grape juice was observed on the basis of the process of degradation of high – molecular substrate by enzymes through the use of Ubbelohd´s viscometer. The research findings provided a lot of knowledge about the occurance of microflora in the process of production of red wine. The commercial preparations added to grape juice played a significant role.