Feeding trials were conducted during 1968 and 1969 to study the physiological effects of Astragalus miser var. serotinus, timber milkvetch, (TMV) in sfieep. In Trial I, TMV was collected from Kamloops and Clinton, pelleted and fed to U groups of Dorset Horn wethers at levels of 0, 35, 70 and 100%. The symptoms exhibited by the sheep fed 100% TMV included backward flexion of the fetlock - joints, hind limb paralysis and inco—ordination of movements. The presence of longitudinal ulcers in the intestines was a characteristic lesion observed in the sheep fed 100% TMV. Fatty infiltration and hemorrhages in the liver and kidney were also observed. After the vetch was cut, dried and pelleted, the miserotoxin content dropped from 2.3 - 3.4% to 0.9%. Trial II was carried out under actual grazing conditions near Kamloops. Yearling ewes fed freshly cut TMV showed symptoms similar to those observed in Trial I. However, the symptoms were less severe and could be observed only when the sheep were exerted. The intestinal ulcers were found to be confined to the jejunum. The activity of the serum glutamic – oxaloacetic transaminase was significantly (p <.05) elevated in the sheep fed TMV indicating extensive tissue destruction. The level of thiamine in blood in both the trials was found to be within the normal range indicating that the beneficial effects of thiamine as a therapeutic agent in TMV poisoning may be indirect. When rats and mice were fed TMV, it was found that they developed a syndrome characterized by the manifestation of convulsive movements terminating in a state of inactivity and a significant decline in body temperature. Extensive hemorrhages were seen in the gastric mucosa. By chromatographic procedures it has been shown that miserotoxin is hydrolyzed to glucose and 3 - nitropropionic acid in the stomach of monogastric animals. In ruminants, miserotoxin is hydrolyzed to glucose and 3 - nitro - 1 - propanol which appears to be responsible for the toxicity. / Land and Food Systems, Faculty of / Graduate

Conventional production of meat products from ruminant animals in the United States requires inputs including the cultivation and nitrogen fertilization of annual grains such as corn and barley, and transportation of cattle and grain to feedlots. Consumers have concerns about the impact of feedlot conditions on animal health, and about the implications of pharmaceutical inputs such as growth hormones and antibiotics on the environment and human health. These concerns have led to a growing interest in pasturefinished meat production by consumers. Such smaller-scale livestock production systems can be healthier and lower-stress for animals, are integrated into local food systems and are more transparent to consumers, and have higher potential profitability for producers than traditional ruminant production methods. There is a strong market for pasture-finished beef products, and prices for naturally or organically raised beef have remained well above feedlot-produced product prices. There is also concern about the impact of ruminant production on the environment, including air and water pollution from feedlot production and greenhouse gasses that are emitted from ruminant animals during feed digestion. This thesis project explored the potential of a beef production system based on perennial legumes, including the non-bloating legume birdsfoot trefoil (BFT; Lotus corniculatus L.) for producing meat products from cattle while reducing concentrate feeding and methane production. The condensed tannins that are produced by BFT bind proteins in the rumen but allow them to be digested in the abomasum and intestines, which in turn leads to better utilization of forage nutrients during the finishing period and higher gains or milk production. The higher digestibility of legumes compared with grasses reduces methane emissions in cattle both through higher digestibility of the forage and through direct impacts on methanogens operating in the rumen. As reported in this thesis, steers finished on BFT gained significantly more weight per day than steers fed another perennial forage legume, cicer milkvetch, but did not gain as rapidly as feedlot-fed steers. At the end of summer grazing, the blood plasma of pasture-fed steers was lower in saturated and omega-6 fatty acids and higher in transvaccenic and omega-3 fatty acids than the blood plasma of feedlot-fed steers. When beef cows grazed grass and legume pastures, enteric methane emissions were lower on the legume pastures than the grass pasture. These results demonstrate that, compared with other feed sources, perennial legume pastures used for cattle production can improve cattle gains and reduce environmental impacts.

Astragalus oniciformis Barneby is a xerophyte of the sagebrush deserts of central Idaho. It is a narrow endemic of the upper Snake River Plains where it inhabits stabilized, aeolian sand deposits over Quaternary basalt flows. The objective of this study was to determine the levels and distribution of genetic differentiation within and among populations of Astragalus oniciformis. Fifteen individuals from each of eight populations, chosen from throughout the range of the species, were selected for their accessibility, density of individuals, and large population size. Two disjunct eastern populations selected for this study have been separated from the continuous western populations for 3600 years by an eight-mile wide, inhospitable lava flow. Inter-simple sequence repeats (ISSR) were chosen as the marker to assess genetic differentiation. Two primers were selected that yielded 40 loci, all of which were polymorphic in A. oniciformis. In an analysis of molecular variance (AMOVA), 88.69 percent of the variation was significantly attributed to variation within populations. The differentiation between the two disjunct populations and the western populations was insignificant. High gene flow (Nm=3.91-3.93) and a low percent deviation from Hardy-Weinberg equilibrium due to population subdivision (G[subscript st]=0.113-0.1134) were found among populations of A. oniciformis. These results suggest that current threats to this species, ranging from plant community changes due to changing fire patterns, habitat alteration from livestock grazing, and habitat loss from agricultural development have not yet affected the genetic diversity of this species. Preservation of the numerous, large populations and the high gene flow will help insure that the levels of genetic diversity found in Astragalus oniciformis will not decrease. / Graduation date: 2002

Astragalus ampullarioides and A. holmgreniorum are two rare endemics of southwestern Utah. Over two consecutive field seasons (2009-2010) we examined pre-emergent reproductive success, based on F/F and S/O ratios, from populations of both Astragalus ampullarioides and A. holmgreniorum, estimated the density of the soil seed bank of A. holmgreniorum as a measure of potential post-emergent reproductive success, and estimated seed persistence within the soil seed bank. Fruit/flower (F/F) ratios and seed/ovule (S/O) ratios varied significantly between populations and among years in both species, and showed low reproductive output in both taxa. In Astragalus ampullarioides F/F and S/O were 0.06±0.01 and 0.16±0.02, respectively (2009), and 0.14±0.01 and 0.41±0.02, respectively (2010). For Astragalus holmgreniorum F/F and S/O ratios were 0.11±0.01 and 0.38±0.02, respectively (2009), and 0.23±0.01 and 0.66±0.02, respectively (2010). Although Astragalus holmgreniorum exhibited a low soil seed bank density (4.3 seeds m-2), seed persistence data showed low a low percentage of seeds germinated during the first year in the soil seed bank. Seeds remaining in the seed bank maintained high percent viability. Soil seed persistence of Astragalus ampullarioides differed from A. holmgreniorum in that a high percentage of seeds germinated during the first year in the soil seed bank. A high percentage of viability in ungerminated seeds was also maintained in A. ampullarioides. Although these species differ in life histories and dependence on soil seed banks, an understanding of the strategies unique to each species will prove useful in management plans.

Rising fertilizer prices have led a return to the use of grass-legume mixtures to reduce N costs and improve pasture productivity. The objective of this study was to determine optimal species combinations of binary grass-legume mixtures to improve forage production and pasture nutritive value in irrigated pastures of the Intermountain West. The study was conducted at the Utah State University Intermountain Pature Research Facility near Lewiston, UT. Tall Fescue (TF), Orchardgrass (OG), and meadow brome (MB) were grown with alfalfa (ALF), birdsfoot trefoil (BFTF), and cicer milkvetch (CMV) in legume-grass mixes and monocultures at planting ratios of 25:75, 50:50, 75:25. Grass monocultures were fertilized with 0 (0 N), 67 (67 N), or 134 kg N ha-1 (134 N). Forage was harvested four times each season during 2011-2012. Forage of the mixtures and monocultures from the first and third harvests was analyzed for crude protein (CP) and neutral-detergent fiber (NDF). Average forage production of the unfertilized TF, MB, and OG monocultures was 11.03, 9.76, and 8.10 Mg ha-1, respectively. TF-ALF, OG-ALF, and MB-ALF grass-legume mixes averaged 24.0, 35.0, and 41.0% higher forage production than their respective unfertilized grass monocultures. The grass-legume mixtures with the highest CP were MB-ALF 159, TF-ALF 159, and TF-OG-159 g kg-1 and average 59, 43 and 51% higher than their respective unfertilized grass monocultures. Likewise, the mixtures with the lowest NDF were OG-ALF 453 g kg-1, OG-BFTF 469 g kg-1, and MB-ALF 480 g kg-1. These mixtures had 10, 7, and 18% lower NDF than their respective unfertilized grass monocultures. Individual harvests had similarly higher yields and CP, with lower NDF for the mixtures than the unfertilized grass monocultures. The grass-legume mixture with the 50:50 planting ratio were most productive and had high forage quality. The grass-legume mixtures had similar forage production as the grass monocultures at 134 kg N ha-1. The grass-legume mixtures also had higher CP and lower NDF than the grass monocultures. Cicer milkvetch did not perform well in irrigated pastures. Grass-legume mixtures with ALF and BFTF can be used to replace commercial N while increasing forage nutritive value.