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MiRNA degradation by a conserved target RNA regulates animal behavior / Dégradation de miARN par une cible ARN conservée régulant le comportement animalBitetti, Angelo 26 September 2017 (has links)
L’objectif de mon projet principal de thèse est de déterminer la fonction biologique d’un lncARN conservés chez le zebrafish que nous avons appelé libra. La séquence de libra étant hautement homologue à la région 3’UTR de la protéine Nrep. Ces deux transcrits, libra et Nrep, contiennent en effet un site de liaison au miARN profondément conservé et inhabituellement complémentaire au miR-29. En utilisant à le modèle souris et les cellules murines, nous avons décrypté la relation régulatrice entre ce transcrit conservé dans l’évolution des vertébrés et la voie métabolique des miARN. Nous avons montré que Nrep limite le domaine d’expression de miR-29 au cervelet, et qu’il le déstabilise en rognant sa séquence. Notre travail révèle donc le premier exemple de dégradation endogène ciblée des miARN (ou TDMD). De plus, un ensemble d’expériences in vivo sur les modèles zebrafish et souris, nous a permis de démontrer que libra et Nrep contrôlent tout les deux le comportement animal. Via la perturbation génétique du site de liaison au miARN de Nrep murin, nous avons observé que ce gène régule le dosage du miR29 de part son site de liaison aux miARN, et que cette régulation est nécessaire à un comportement animal normal. Dans la seconde partie de ma thèse, je décris une stratégie exploré afin de déréguler les lncARN de la manière la moins invasive possible. Les lncARN sont actuellement neutralisés par des approches qui introduisent de vastes changements de séquence au niveau génomique. Nous avons donc développer une stratégie in vivo, appliquée au zebrafish, qui inactive les lncARN via l’insertion génomique d’une séquence ribozyme autoclivante ou d’un signal polyA prématuré. / The goal of my main thesis project was to determine the biological function of a deeply conserved zebrafish long noncoding RNAs (lncRNA) which we called libra. libra shows sequence similarity with the 3'UTR of the NREP a protein coding transcript. Both libra and Nrep contain a deeply conserved and unusually complementary microRNA (miRNA) binding site for miR-29. Using both the mouse model and mouse cell lines, we deciphered the regulatory relationship between this conserved transcript and the miRNA pathway. We showed that Nrep restricts the spatial expression domain of miR-29 in the cerebellum and that it destabilizes miR-29 through 3' trimming. Until now, only viral transcripts and artificial reporters engineered to contain highly complementary miRNA binding sites have been shown to regulate miRNAs in this fashion. Thus, our work uncovers the first example of endogenous target-directed miRNA degradation (TDMD). In addition, through a set of in vivo experiments in zebrafish and mouse, we showed that both libra and Nrep control normal animal behavior. By genetically disrupting the miR-29 binding site in Nrep in mouse, we showed that Nrep regulates miR-29 dosage through its miR-29 site and controls animal behavioral. In a second part of my thesis I describe a strategy to genetically downregulate lncRNAs in a minimally invasive manner. Approaches to knock-out lncRNAs that do not introduce vast sequence changes at the genomic level have not been adequately developed yet. I present our in vivo strategy applied to the zebrafish model using a genomic knock-in of a self-cleaving ribozyme sequence and a premature poly(A) signal to knock-out lncRNAs.
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Notch Regulation of Adam12 Expression in Glioblastoma MultiformeAlsyaideh, Ala'a S. 01 January 2012 (has links) (PDF)
Glioblastoma is the most common malignant brain tumor, accounting for 17% of all primary brain tumors in the United States. Despite the available surgical, radiation, and chemical therapeutic options, the invasive and infiltrative nature of the tumor render current treatment options minimally effective. Recent reports have identified multiple regulators of glioblastoma progression and invasiveness. It has been demonstrated that ADAM12, A Disintegrin And Metalloproteinase encoded by ADAM12 gene, is over-expressed in glioblastoma and directly correlated with tumor proliferation. Additionally, dysregulation of the Notch signaling pathway has been implicated in the pathogenesis of many gliomas. Lastly, an evolving role of microRNAs, small noncoding RNAs, in carcinogenesis is progressively growing. A recent study has identified ADAM12 as a notch-related gene, and another demonstrated that inhibition of notch signaling decreased glioblastoma recurrence. However the mechanisms of regulation are still unknown. In this study, we hypothesize that direct downregulation of microRNA-29, downstream of over-expression of notch enhances glioblastoma malignancy through upregulation of ADAM12. Although our data demonstrate upregulation of Notch1, its downstream target HES1, and ADAM12 in U87MG glioblastoma cell line. Expression of the cleaved intracellular Notch1 was not detected. Furthermore, we were unable to demonstrate an inhibitory effect of ɣ-secretase inhibitor on Notch signaling, likely reflecting the requirement for modifying culturing conditions or detection in our assays. Furthermore, miR-29 was detected in glioblastoma cells. The expression of miR-29 was further elevated by ɣ-secretase inhibitor treatment, suggesting a role for Notch1 inhibition on miR-29 expression. Although no conclusive results are shown in our work, a role of Notch1 through miR-29 is implicated in the pathogenesis of glioblastoma pathogenesis warranting further investigation into the role downstream target genes in the Notch signaling pathway.
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