Context: Dorsiflexion range of motion (DFROM) is often limited in patients with chronic ankle instability (CAI). Whole-body vibration (WBV) may enhance DFROM by helping to reposition the talus and assisting with talocrural arthrokinematics. Objective: To determine if WBV can enhance DFROM in patients with ankle instability and determine if talar position is affected. Setting: Cohort study. Patients or Other Participants: A total of 25 subjects with CAI (17 women, 8 men; age = 22 ±2.101 years, mass = 72.4 ±17.9 kg, height = 171.2 ± 11.6 cm) participated. Intervention(s): Participants in the WBV group completed a 4-week (12 session) WBV program consisting of 6 sets of 30 s at 35 Hz High amplitude with 30 s rest in between standing on a 30° slant board. Participants in the dorsiflexion (DF) group completed a 4-week program without WBV consisting of 6 sets of 30 s standing on a 30° slant board with 30 s rest in between. Participants in the control (C) group did not receive any intervention. Main Outcome Measure(s): Lateral talus position via radiographic imaging, non-weight-bearing (NWB) DFROM, and weight-bearing (WB) DFROM were assessed preintervention, immediately postintervention, and 24 hours after the final intervention. Results: No significant change was detected in talus position measured by X-ray (F = 1.561; p < .05). NWB DFROM (F = 1.543; p < .05) and WB DFROM (F = .774; p < .05) measurements also did not result in significant changes after the WBV program. Conclusions: Four weeks of WBV treatments did not improve talus position or DFROM in ankle instability subjects.