Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-08-22T18:10:01Z No. of bitstreams: 1 2016 - Kerly Martinez Andrade.pdf: 5719602 bytes, checksum: 90b1873e2b692ed4bcabe9969709919b (MD5) / Made available in DSpace on 2017-08-22T18:10:01Z (GMT). No. of bitstreams: 1 2016 - Kerly Martinez Andrade.pdf: 5719602 bytes, checksum: 90b1873e2b692ed4bcabe9969709919b (MD5) Previous issue date: 2016-09-05 / CAPES / The Atlantic Forest is one of the ecosystems richest in biological diversity of the planet and is directly responsible for the quality of life of thousands of Brazilians. Among the species present in the Atlantic including PNM Curi?, Paracambi, R.J., are present cercosporoid fungi, represented by the genus Cercospora. Are plant parasitic fungi, polyphagous and cosmopolitan, causing leaf spots, as well as in flowers, fruits and cereal seeds, vegetables, ornamental plants of forest trees and grasses. These fungi were quite challenged in recent years, mainly because of its immense morphological variation and studies based on nucleic acid sequences, have become widely used for the elucidation of several doubts in its taxonomy, then for the knowledge and description of new species in new hosts are so important to discuss their morphology as well as studies using molecular data for this group. This work was developed aiming to increase the knowledge of biodiversity of cercosporoid fungi associated to different botanical families from the Atlantic Forest in the state of Rio de Janeiro. From October, 2014 to May, 2016 several collections of plants with leaf spot symptoms were collected in Atlantic Forest areas, especially in the PNM Curi?, Paracambi, RJ. and areas of the municipality of Serop?dica, RJ. and taken to DENF laboratories (Mycology) / UFRRJ, and studied using optical, dissecting and scanning electron microscope techniques and several attempts of isolation in axenic culture media were made, for their in vitro characterization and extraction of DNA for molecular characterization studies. Collection of material was done at five different times in PNM Curi? and several other sporadic within the campus UFRRJ and Fazendinha EMBRAPA Agrobiology - Serop?dica. It was about 150 samples, of which 22 species are presented in this work, in Cercospora genres (2 species), Passalora (2 species) and Pseudocercospora (18 species). Ten species were isolated in PDA medium and had their molecular analysis performed with ITS (IT1-5.8S-ITS2). Based on morphometric data are proposed 21 likely new species and a known one found in B. nivea, which was confirmed with molecular data. These species will be presented as well as its taxonomic position discussed. / O bioma Mata Atl?ntica representa um dos ecossistemas com maior riqueza em diversidade biol?gica do planeta e ? diretamente respons?vel pela qualidade de vida de milhares de brasileiros. Entre as esp?cies presentes na Mata Atl?ntica incluindo PNM Curi?, Paracambi, R.J., est?o presentes os fungos cercospor?ides, representados pelo g?nero Cercospora. S?o fungos fitoparasitas, pol?fagos e cosmopolitas, causadores de manchas foliares, em flores, frutos e sementes de cereais, hortali?as, plantas ornamentais, de ess?ncias florestais e de gram?neas. Estes fungos foram bastante questionados nos ?ltimos anos, principalmente pela sua imensa varia??o morfol?gica e estudos baseados em sequ?ncias de ?cidos nucleicos, tornaram-se amplamente utilizados para a elucida??o de v?rias d?vidas taxon?micas logo, ? importante o conhecimento e descri??o de novas esp?cies em novos hospedeiros e discutir sua morfologia bem como realizar estudos com dados moleculares para este grupo. Este trabalho foi desenvolvido com o objetivo de ampliar o conhecimento da biodiversidade f?ngica de cercosp?roides associada a diferentes fam?lias bot?nicas representadas da Mata Atl?ntica, no Estado do Rio de Janeiro. Durante o per?odo de outubro de 2014-maio 2016, 150 plantas com sintomas de manchas foliares foram coletadas em ?reas de Mata Atl?ntica, em especial no PNM do Curi?, Paracambi-RJ, e ?reas do munic?pio de Serop?dica, RJ. Foram levados aos laborat?rios do DENF (Setor de Micologia)/ICBS/UFRRJ e estudados usando t?cnicas de microscopia ?ptica, estereosc?pica e eletr?nica de varredura. V?rias tentativas de isolamentos em meio ax?nico foram efetuadas, visando sua caracteriza??o in vitro e extra??o de DNA para estudos de caracteriza??o molecular (laborat?rios da EMBRAPA Agrobiologia). Foram efetuadas cinco coletas em diferentes ?pocas no PNM Curi? e v?rias outras espor?dicas dentro do Campus da UFRRJ, bem como Fazendinha da EMBRAPA Agrobiologia ? Serop?dica. Totalizaram-se cerca de 150 amostras, das quais 22 esp?cies foram estudadas e s?o apresentadas neste trabalho, nos g?neros Cercospora (2 esp?cies), Passalora (2 esp?cies) e Pseudocercospora (18 esp?cies). Dez esp?cies foram isoladas em meio BDA e tiveram sua an?lise molecular efetuada com ITS (IT1-5.8S-ITS2). Com base em dados morfom?tricos, s?o propostas 21 prov?veis novas esp?cies e uma j? conhecida encontrada em B. nivea, o qual teve a confirma??o com dados moleculares. Estas esp?cies ser?o apresentadas, bem como sua posi??o taxon?mica discutida.