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The task of the experiment was to confirm or reverse the positive and negative effects of mycorrhizal fungus on morphological changes in selected vegetable representatives. Its main part consisted of verification of these influences in the plant of concrete representatives, and other secondary experiments followed. Representatives for this task were Paprika Annual (Capsicum annuum) and Tomato Plant (Lycopersicon lycopersicum). The bulk of the verification took place at the Faculty of Horticulture at Mendel University in Brno. It has been found that the application of mycorrhizal fungus can influence certain quantitative parameters of selected vegetable representatives.

The aim of this thesis was to verify whether it is possible to use mycorrhizal symbiosis in nursery production of selected varieties of highbush blueberries (Vaccinium corymbosum). In the theoretical part, a literature review on a given topic was elaborated. The methodology of the experiment was elaborated within the practical part. Practical experiments were carried out on experimental plots of the Institute of Breeding and Propagation of Horticultural Plants in Brno during 2018. During the growing season, growth indicators, physiological indicators, root system size and the degree of root colonization by mycorrhizal structures were evaluated. The results of my work clearly demonstrate the effect of mycorrhizal symbiosis on resistance to biotic and abiotic factors, especially drought stress. However, it should be noted that it would be useful to devote more time to researching ericoid mycorrhiza and to monitor more indicators to help us understand it better.

This diploma thesis deals with usage of arbuscular mycorrhiza in the reproduction of selected varieties of gooseberries in plant nursery. The experiment was established in Brno in autumn 2018 and was completed in spring of the following year. In total, 60 plants of the ´Black Negus´, ´Carat´ and ´Rolonda´ varieties were evaluated, with half of the plants being inoculated with mycorrhizal fungus and the other half without mycorrhiza, as control plants. The following parameters were evaluated: extent of root colonization by mycorrhizal structures, content of chlorophyll, relative size of the root system, dry matter in the root system, substrate temperature and substrate humidity, root collar diameter and shoot length. The results show that inoculation with mycorrhizal fungus influenced the plant root system in positive way, which was reflected in the increase in root collar diameter. The inoculation with mycorrhizal fungus should also have a positive effect on the growth of the aerial part of the plant, the health and plant resistance to drought and pathogens.

The aim of my diploma thesis was to summarise all possible information of using of preparations with micro-organisms and helpful soil preparations. In the experimental part the aim was to prove the influence of these preparations on production and nutritional parameters of the leek (Allium porrum L.) in the soil very poor with nutrients. As the type of the leek the autumn kind Gevaria F1 was chosen. On the model cultivation of vegetables, I observed the influence of the preparation with mycosis fungi Glomus intraradices BEG140, G. claroideum BEG210, G. microaggregatum BEG56, G. intraradices S7, G. versiforme along with tribe Trichoderma harzianum 78. Then there was also the preparation which contained the extract from alga and bacteria Azospirillum brasilense K2012 242/9 NCAIM (P) B.001403, Azospirillum largimobile K2012 B41 NCAIM (P) B.001402, Leuconostoc mesenteroides K2009 25/4 NCAIM (P) B.001372, Pseudomonas chlororaphis K2009 13/4 B NCAIM (P) B.001370, Pseudomonas lundensis K2009 9/4-B NCAIM (P) B.001371 and a substance Fenyl-7, which is artificial similarity of strigolaktons. The experiment was done on the grounds of the company Richter Rasen Slovakia s.r.o. . From the evaluated results we can see that the use of preparations did not have any crucial influence on production parameters of the leek. The highest production was at the checking version. At the others determined parameters of the production, the highest value was at the checking version. However, the differences were not statistically significant. The highest amount of solids was found at the version containing mycosis fungi and trichoderm. I evaluated also the antioxidant kapacity; the highest measured value was in the checking plants comparing to the lowest amount at the version of alga and a bacteria and the preparation Fenyl -- 7. The content of nitrates in the plant netting was the highest at the checking version. The content of vitamin C had the highest value at the chceking version and the lowest value at the version with mycorrhiza and trichoderm. The rate of colonisation was 70,1 % at the version with mycorrhiza; the checking version in this evaluation got 25,65 % of the rate of colonisation of the root. From the elaborated results the conclusion that the preparations had the impact on the evaluated parameters in case of testing in the poor sandy soils cannot be clearly done.

In the diploma thesis the literature dealing with the comparison of root colonization by rhizosphere microorganism in the conventionally and ecologically treated vineyards was collected and studied. The soil samples and the parts of the vine roots from the vineyard of the wine growing Gotberg and vineyard Kačírky were took and analyzed. These samples were used for the survey of root colonization intensity by arbuscular rmycorrhizal fungi and they were also used for determination of the microorganism quantity of the selected physiological groups in the soil. The occurrence of the arbuscular mycorrhiza was studied under an electric light microscope and the result was expressed as a percentage. To extract the microorganisms from the environment the dilution method with the subsequent smear onto agar plates with a suitable medium was used. The results show the higher colonization by arbuscular mycorrhizal fungi in the conventionally cultivated vineyard. However, the occurrence of the soil microorganism, for example, the total amount of microorganisms was in the bio vineyard higher.

The thesis deals with quantitative characterization of glomaline in three localities (Hrušky, Bošovice and Zástřizly). We determine easily extractable glomalin by using Bradford´s method. We analyzed the dependence of glomaline on pH and physiological availability of soil nitrogen nitrogen (N/B). The glomalin content was relatively low but in view of the fact we analysed arable land it is standard. Furthermore we also reported that glomalin content was decreasing with soil depth. All these results correlate with other scientific publication. Our results show that glomalin content is one of the most sensitive biological parametr which response to soil disturbance.

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