No description available.

This diploma thesis deals with the influence of mycorrhizal preparations in the reproduction of ornamental trees and their recommendations for the production in growing nurseries. Two species of shrubs were chosen for the experimental part, Deutzia gracilis 'Nikko' and Weigela florida 'Piccolo'. The shrubs were propagated by herbal cuttings. The experiment took place in 2018 on the site of the Faculty of Horticulture in Lednice. After laboratory evaluation, the root colonization by arbuscular fungi has been demonstrated. The degree of colonization has not reached high levels, but we have seen their gradual increase. From the results it is noticeable that the preparations used did not influence the development of adventitious roots and the vitality of cuttings so it is not possible to label it as effective and recommending for the production in growing nurseries. It should be noted that the duration of this experiment was only one year.

The theoretical part is devoted to the production of vermi compost, earthworm breeding and arbuscular mycorrhizal symbiosis. The practical part of the procedure followed in establishing containter experimentwith lettuce (Lactuca sativa), further evaluation and processing of results in tables and graphs. The aim was to experimentally determine the effect on plant growth vermi compost. Furthermore, it aims to validate the influence of colonization root mycorrhizal fungi. Effect of vermi compost to an average dry weight of aboveground and underground materials was confirmed and significant difference was observed. Vermi compost had also an influence on the length outer root root hyphae per cm2 membrane filter. Impact on the percentage of root colonization was clearly confirmed. In addition, as of Symbivit mycorrhizal positive influence on above and below ground biomass and the length outside the root hyphae. Impact on the percentage of root colonization was not significantly manifested.

No description available.

This dissertation deals with the use of mycorrhizal symbiosis in the cultivation of vegetables and aims to increase the yield and quality. The literature review defines different types of the mycorrhizal symbiosis. Furthermore, the thesis describes the arbuscular mycorrhizal symbiosis (AM), its biology, and influence of mineral nutrition and impact of biotic and abiotic stresses. This description is complemented by the prospects of the AM symbiosis in agriculture. The experimental part is devoted to the evaluation of plants inoculated with arbuscular fungi. The experiment was conducted as a pot experiment in containers (2009 - 2011) with appropriate substrate and irrigation dose. In parallel, there were a few other experiments with vegetable seedlings. The evalueted parameters included: yield parameters and also nutritional parameters such as vitamin C, total antioxidant capacity and dry matter content. Very significant indicator was the assessment of mycorrhizal root colonization by the fungus. There were used different types of arbuscular fungi, as well as the combination of AM fungi with saprophytic fungi. The results were statistically processed. Based on the results, we can conclude that mycorrhiza resulted in an increase in yield of tomatoes and leeks grown in containers. There was no statistically significant effect of any mycorrhizal treatment on the dry mater content. Positive effect of inoculation was shown at vitamin C content and total antioxidant capacity. Tomato and lettuce seedlings were also positively influenced by AM fungi. Overall, the experiment can be considered as successful, because the positive effect of mycorrhizal symbiosis was proved.

The two-year experiment (2010, 2011) dealt with the evaluation of yield and quality parameters of vegetables Capsicum annuum L., variety 'SLÁVY F1' and Allium porrum L., 'GIGANTE SUIZO' treated with mycorrhizal product SYMBIVIT in two irrigation modes. The research was conducted as a field experiment. The site was located on the properties of Faculty of Horticulture MENDELU, Mendeleum, Lednice. The positive effect of the mycorrhizal preparation as an intensifying factor was statistically proven in the quality of pepper seedlings. The set parameters were: plant height, stem diameter and increase of the number of leaves. The set parametres in the quality of leek seedlings were: stem diameter, length of the above-ground part, the length of the underground section, weight of fresh roots, weight of roots after drying. The positive effect of the mycorrhizal preparation as an anti-stress measure was proven in the evaluation of yield of peppers in 2011. The positive effect of the mycorrhizal preparation as an anti-stress measure was also proven in the evaluation of yield of the 1st grade quality of peppers in 2011 and in the quantity of nitrates in leek planted in 2010. The positive effect of mycorrhizal preparation as an intensifying factor or as an anti-stress measure on the other evaluated parameters was not proven.

Fertilization and liming of grasslands have been used as an instrument improving hay production for centuries. For a long period of time, these practices not only sustained and increased hay yields, but also had negative effects on grassland ecosystems. Among others, they caused considerable changes in plant species composition leading in most cases to the reduction of species richness due to a lower capability of indigenous plants to compete with more productive pasture species. Furthermore these practices caused changes in soil and biomass chemical properties and shifts of soil microbial communities, including arbuscular mycorrhizal fungi. The aim of this thesis was to analyze the responses of plant and mycorrhizal community and soil chemical properties in grassland ecosystems to long-term application of nitrogen, phosphorus, potassium, and calcium. Results of this work indicate that agricultural management practices aimed at maximization of hay production may, in the long run, significantly increased hay yield, reduce plant species richness, promote grasses but suppress herbs, legumes and arbuscular mycorrhizal fungi in the soil although the response to phosphorus varied among different arbuscular mycorrhizal fungal taxa. Long-term fertilization also significantly changed soil chemical properties and considerably reduced belowground carbon storage.

This diploma thesis deals with usage of arbuscular mycorrhiza in the reproduction of selected varieties of gooseberries in plant nursery. The experiment was established in Brno in autumn 2018 and was completed in spring of the following year. In total, 60 plants of the ´Black Negus´, ´Carat´ and ´Rolonda´ varieties were evaluated, with half of the plants being inoculated with mycorrhizal fungus and the other half without mycorrhiza, as control plants. The following parameters were evaluated: extent of root colonization by mycorrhizal structures, content of chlorophyll, relative size of the root system, dry matter in the root system, substrate temperature and substrate humidity, root collar diameter and shoot length. The results show that inoculation with mycorrhizal fungus influenced the plant root system in positive way, which was reflected in the increase in root collar diameter. The inoculation with mycorrhizal fungus should also have a positive effect on the growth of the aerial part of the plant, the health and plant resistance to drought and pathogens.

The topic of the diploma thesis was the study of the influence of commercial mycorrhizal preparation on the rooting of softwood cuttings of two selected species of ornamental shrubs (Forsythia × intermedia 'Maluch' and Weigela florida 'Piccolo'). The main objective was to find out whether the mycorrhizal preparation can make the process of vegetative propagation more efficient and favourably influence the formation of roots. The used preparation contained arbuscular mycorrhizal fungi of the genus Glomus, two different amounts of the preparation were used. The experiment was carried out in the summer months of the year 2016. The experiment was founded in a plastic greenhouse on the ground of the Faculty of Horticulture in Lednice in Moravia (the Czech Republic), two main terms of propagation passed (in June and August) and one supplementary term passed (in August). The total number of adventitious roots, the number of branched roots, the length of shoots and the mortality of cuttings were evaluated. Mycorrhizal colonization of roots was evaluated in the laboratory under the microscope. The results of the experiment did not show that the used mycorrhizal preparation can positively affect the rooting of softwood cuttings, the application of the mycorrhizal inoculum did not improve the efficiency of vegetative propagation and did not provide convincing advantages. Mycorrhizal fungi did not stimulate the growth of cuttings, and in fact they did not influence the number of adventitious roots neither in Forsythia × intermedia 'Maluch', nor in Weigela florida 'Piccolo'. The only significant positive was that the Weigela florida 'Piccolo' had a higher number of branched roots after inoculation. The observation of roots under the microscope showed that the roots of both species were colonized by arbuscular mycorrhizal fungi, but the extent of colonization did not reach high levels. Mycorrhizal inoculation of propagation substrate cannot be recommended as an effective method that would improve rooting. However, it should be added that the experiment only passed for one year and the results were based only on one term of propagation. Thus the influence of mycorrhizal fungi on the rooting of plant cuttings should be the subject of further research.

The aim of this diploma thesis was to determine the impact of mycorrhizal fungi on the growth parameters of model vegetable crops. As a model crops were selected Cucumber (Cucumis sativus L.) and Tomato ( Lycopersicon esculetnum Mill.). There were selected these three sorts of arbusko - mycorrhizal fungi to inoculate the medium were the crop seeds were sown: genus Glomus. Glomus claroideum BEG210, Glomus intraradices BEG140 a Glomus mosseae BEG95. The experiment that was conducted on this diploma thesis took place partially in the glasshouse as well as in the climate chamber, both in Horticulture Faculty in Lednice, Mendel University in Brno. The evaluation of treatment impacts was focused on the growth parameters such plant size, the width of the root collar and the leaf area of plants. Besides these parameters was also compared the plants 'weight. The results were statistically analyzed using STATISTICA 12 and are also included in the thesis. From the results we can conclude that the positive effect of mycorrhiza was reflected in both plant size and the weight of above-ground parts, which means an increase in fresh biomass. For these parameters, there were statistically significant differences in compare to control variation. Mycorrhizal treatment had also a positive effect on the growth of leaf area, and thus the photosynthetic apparatus of plants was increased. These results were supported by statistically significant differences compared to the control, as well. So this means that the mycorrhizal treatment has a very positive effect on plants.