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Molecular genetic analysis of chemical-induced sporulation of Myxococcus xanthusChatwin, Heather M. January 1994 (has links)
No description available.
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A membrane active antibiotic from Cystobacter spYasouri, Fazel Najafi January 1990 (has links)
No description available.
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A molecular investigation of light-induced carotenoid synthesis in Myxococcus xanthusBrowning, Douglas Frame January 1997 (has links)
No description available.
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Towards a molecular mechanism for light induction of gene transcription in Myxococcus xanthusBerry, Andrew Edward January 1997 (has links)
No description available.
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A Partial Copy of msDNA From a New Retron Element Is Likely a Retrotransposed DNA Found in the Myxobacterium Nannocystis exedensLampson, Bert C., Xu, Chunying, Rice, Scott A., Inouye, Sumiko 16 October 2002 (has links)
Retrons are genetic elements encoding reverse transcriptase (RT) usually located on the chromosome of a wide variety of mostly Gram-negative bacteria. Here we describe a new retron, designated Ne144, found in the chromosome of the myxobacterium Nannocystis exedens. This element codes for a 515-amino-acid RT that is most closely related to those found in other myxobacterial retrons. The RT is responsible for the production of a small satellite DNA called msDNA. This msDNA is composed of a 144 base, single-stranded DNA that is linked to a 72 base single-stranded RNA. The RNA strand is joined to the 5′ end of the DNA chain via a 2′-5′ linkage that occurs from the 2′ position of an internal guanosine residue in the RNA. In addition to the retron element, the chromosome of N. exedens also contains several partial copies of the msDNA sequence as revealed by DNA hybridization experiments using msDNA as a probe. One of these partial copies was characterized from a chromosome restriction fragment and found to contain a sequence that matches the last 82 bases of the DNA strand and five bases of the RNA strand in msDNA-Ne144. This partial copy of msDNA is very likely a retrotransposed sequence that was generated by reverse transcription using an RNA (the primer-template RNA for msDNA) as a template and the 3′ end of a nick in the chromosome as a primer, followed by incorporation into an open reading frame. The presence of this truncated copy of msDNA is strong evidence of retrotransposition in N. exedens causing an alteration in the bacterial genome.
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Mathematical modelling of motility regulation in Myxococcus xanthusChen, Yirui 11 January 2024 (has links)
Myxococcus xanthus, referred to as a 'social bacterium', demonstrates unique behaviors such as coordinated motility, cooperative feeding, and multicellular structure formation. Its complex social behaviors and developmental processes make M. xanthus a model organism for studying bacterial social behaviors and their underlying mechanisms. Much of the social behavior of M. xanthus hinges on coordination of cell motility among bacteria in close proximity. M. xanthus moves on moist solid surfaces, using its Adventurous (A)-motility and Social (S)-motility systems. A striking feature of M. xanthus motility is the periodic reversal of its direction of movement. The reversal frequency is influenced by chemical and mechanical cues in the surrounding environment. The modulation of the reversal frequency upon physical contact between cells is believed to be a key factor in the bacterium's social behaviors, especially in the formation of complex patterns and structures within the cell population.
Here I utilized mathematical modeling to study the motility regulation in M. xanthus, focusing on contact-dependent reversal control, mechanosensing response and impact of motility regulation in solitary (single-cell) predation. My goal is to provide experiment-guiding theories and hypotheses for M. xanthus motility regulation, which is essential to fully understand the social behaviors in this bacterium.
In Chapter 2, I developed a single-cell model based on a hypothesis that the motility regulation in M. xanthus is mediated by the interplay between the cell polarity regulation pathway and the A-motility machinery. The aim of this model is to elucidate the cellular mechanism governing contact-dependent motility coordination among cells and to understand how contact-dependent responses at the single-cell level contribute to population-level patterns. This model suggests that the A-motility machinery of M. xanthus potentially serves as a 'mechanosensor' that transduces mechanical cues in the environment into a reversal modulation signal.
Chapter 3 addresses a puzzling observation: cells with A-motility alone (A+S−) show a dependence of reversal frequency on substrate stiffness that is opposite to what is observed in wild-type cells that possess both motility systems. Specifically, A+S− cells reverse less frequently on harder substrates, whereas wild-type cells reverse more frequently. To elucidate this perplexing phenomenon, I refined the single-cell model developed in Chapter 2 to study the mechanosensing behaviors with or without S-motility. The base model was sufficient to explain the mechanosensing response in A+S− cells. I then proposed possible interactions between the A-motility and S-motility systems that could explain the contrasting responses to substrate stiffness when S-motility is present or absent. This provides a testable prediction for future experimental investigations. The model suggests that the A-motility system in M. xanthus functions as a central hub of mechanosensing-based reversal control, modulating cell reversal in response to environmental mechanical cues.
In Chapter 4, I constructed an agent-based model to investigate the optimal motility strategies for nutrient consumption by M. xanthus during its solitary predation. For different nutrient source types and their uptake latencies, the model identifies 'explore', 'inch', and 'fast explore' as the three most effective motility strategies. Variability in velocity and cell reversal period changes the optimal strategies from 'explore' mode to 'revisit' mode and to 'speed-controlled explore' mode, respectively, for massive remains of prey nutrient sources with moderate uptake latency. The experimental observation that solitary M. xanthus cells combined the 'revisit' and 'inch' mode—as predicted by the model for nutrient acquisition respectively from prey remains and macromolecules—suggests that some of the dead preys may not release its cellular contents immediately and that release of molecular nutrients may require multiple digestion cycles. This model provides insights into the functional role of complex motility regulation in M. xanthus during solitary predation. / Doctor of Philosophy / A fundamental question in biology is how a cell responds to physical, chemical and biological stimuli. Such responses are usually mediated by complex coupling between multiple cellular processes. Bacterial motility and its regulation present many excellent examples of this kind. This dissertation focuses on Myxococcus xanthus, a model organism for bacterial social behavior due to the highly coordinated motility of cells in M. xanthus colonies and their functional cooperation. In this dissertation, I built theoretical models to study the motility regulation in M. xanthus, which is essential for understanding the social behaviors and survival in this bacterium. The specific focuses are to comprehend how environmental mechanical cues regulate M. xanthus's motility, and how the observed motility regulation in M. xanthus facilitates its predatory behavior at the single-cell level. The key aspect of this work is to construct a modeling framework to provide coherent explanations for the experimental observations. It is anticipated that the hypotheses generated through modeling will guide new experiments in the field of myxobacterial biology. The findings offer general insights into how bacterial cells sense, respond, and adapt to the chemical, physical, and biological cues.
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Natural products from Myxococcales and Bacillales & Description of a new myxobacterial taxonSood, Sakshi 14 March 2014 (has links)
No description available.
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