Made available in DSpace on 2014-12-17T15:32:21Z (GMT). No. of bitstreams: 1 ClarissaFD_DISSERT.pdf: 1047575 bytes, checksum: 1162ac27915144b24bb631aefd3e421b (MD5) Previous issue date: 2012-02-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The Epidermoid Carcinoma (EC) is the most common lesions located in the region of the head and neck and, despite advances in treatment modalities, the prognosis is still poor. The malignant cells show an increase in glucose uptake, process mediated by glucose transporters (GLUTs). Increased expression of GLUT 1 and GLUT 3 is related to the aggressive behavior of this lesion. The aim of this study was to evaluate, through immunohistochemistry, the expression of GLUTs 1 and 3 in EC of the lower lip. The sample consisted of 40 cases of EC of the lower lip, of which 20 had regional lymph node metastasis and the remaining 20 with absence of metastasis. The percentages of immunostained cells in front of tumor invasion and in the center of tumor were evaluated. These results were related to the presence and absence of lymph node metastasis, TNM classification and histological grading. The percentage of cytoplasmic/membranous expression of GLUT 1 ranged from 77.35% to 100%, while for GLUT 3 this value ranged from 0.79% to 100%. As for nuclear staining for GLUT 1, this percentage ranged from 0 to 0.42%, however. GLUT 3 showed only one case with nuclear staining. Despite the significant expression of tumor cells related to the proteins studied, we observed no statistically significant relationship between the variables and the antibodies analyzed, regardless of the region evaluated. However, there was a moderate positive correlation between cytoplasmic/membranous immunoexpressions of GLUT 1 in invasion front and in the tumor center (r = 0.679, p <0.001). Similarly, moderate positive correlation was found between the nuclear immunoexpressions of GLUT 1 in the invasion front and in the tumor center (r = 0.547, p <0.001). For GLUT 3, was also observed a moderate statistically significant positive correlation between cytoplasmic/membranous expression in tumor invasion front and in tumor center (r = 0.589, p <0.001). We also observed that the immunoreactivity for GLUT 1 was higher than GLUT 3 expression in invasion front (p <0.001) and tumor center (p <0.001). From these results, this study suggests that tumor hypoxia is a remarkable characteristic of the EC of the lower lip and GLUT 1 may be primarily responsible for glucose uptake into the interior of the malignant cells / O Carcinoma epiderm?ide (CE) est? entre as les?es mais comuns localizadas na regi?o de cabe?a e pesco?o e apesar dos avan?os nas modalidades de tratamento, o progn?stico ainda ? pobre. As c?lulas malignas apresentam um aumento na absor??o de glicose, processo esse mediado pelos transportadores de glicose (GLUTs). O aumento da express?o de GLUT 1 e GLUT 3 encontra-se relacionado com o comportamento agressivo dessa les?o. O objetivo desse estudo consistiu em avaliar, atrav?s de estudo imuno-histoqu?mico, a express?o dos GLUTs 1 e 3 em CE de l?bio inferior. A amostra foi composta por 40 casos de CE de l?bio inferior, dos quais 20 apresentavam met?stase linfonodal regional e os 20 restantes com aus?ncia de met?stase. Foram avaliados os percentuais de c?lulas imunomarcadas no front de invas?o e no centro do tumor, esses resultados foram relacionados com a presen?a e aus?ncia de met?stase linfonodal, estadiamento cl?nico TNM e grada??o histol?gica. O percentual de marca??o citoplasm?tica/membranar para GLUT 1 variou de 77,35% a 100%, j? para GLUT 3 esse valor variou de 0,79% a 100%. Quanto ? marca??o nuclear para GLUT 1, este percentual variou de 0 a 0,42%, no entanto, GLUT 3 apresentou apenas um caso com marca??o nuclear. Apesar da expressiva marca??o das c?lulas tumorais frente aos marcadores estudados, n?o foi poss?vel observar rela??o estatisticamente significativa entre as vari?veis estudadas e os marcadores analisados, independente da regi?o avaliada. Contudo, foi observada moderada correla??o positiva estatisticamente significativa entre as imunoexpress?es citoplasm?tica/membranar de GLUT 1 no front de invas?o e no centro do tumor (r=0,679; p<0,001). De forma similar, foi constatada moderada correla??o positiva entre as imunoexpress?es nucleares de GLUT 1 no front de invas?o e no centro tumoral(r=0,547; p<0,001). Para GLUT 3, tamb?m foi observada moderada correla??o positiva entre as imunoexpress?es membranar/ citoplasm?tica dessa prote?na no front de invas?o e no centro tumoral (r=0,589; p<0,001). Foi observado, ainda, que a imunoexpress?o de GLUT 1 em rela??o a GLUT 3 foi maior tanto no front de invas?o (p < 0,001) como no centro do tumor (p<0,001). A partir desses resultados, conclui-se que a hip?xia tumoral ? uma caracter?stica marcante no CE de l?bio inferior e GLUT 1 pode ser o principal respons?vel pela capta??o de glicose para o interior das c?lulas malignas