Goldfish, an exotic originally from Asia, are frequently released into North American waters, causing unknown impacts on native fauna and flora. In the wild, diet overlap between feral goldfish (Carassius auratus) and native red-spotted newts (Notophthalmus v. viridescens) is high, leading to potential interspecific competition over food. In the laboratory, we manipulated densities of goldfish and red-spotted newts to test competition for benthic food organisms between these unrelated species. / No intraspecific interference was noted for either goldfish or newts at any density. However, as density increased food resources were more rapidly depleted by both species, suggesting scramble competition. Foraging strategies of the species differed. Goldfish shoaled more and abandoned food patches before they were depleted, whereas newts were solitary and exhausted a food patch before moving on to new sites. Both species found more food patches when with heterospecifics than with conspecifics, suggesting interspecific information transfer. In addition, goldfish found more food patches than newts in all trials, indicating scramble competition occurred.

Thesis (Ph. D.)--University of Toronto, 2004. / Includes bibliographical references.

No description available.

<u>Diplostomulum trituri</u> is associated with pathological damage which includes increased size of the ventricles and loss of neural tissue. Neurophysiological symptoms of parasitism include sluggish behavior and poor reflexes. Black material was observed in the lumen of the intestinal ceca. Several mesocercariae were observed to be attached to the neural tissue by their oral sucker. In 150 newts examined from Mountain Lake, Giles County, Virginia, the incidence of infection with <u>D. trituri</u> was 100 percent. The total number of parasites in the brain and surrounding area ranged from 2 to 300. The number of mesocercariae in the ventricles of the brain was approximated to range from 5 to 30. The attempts to recover the encysted larval stage and/or adult failed using Japanese quail, mallard ducks, raccoon, muskrat, opossum, domestic cat, and fish from families Salmonidae, Catostomidae, Cyprinidae, and Centrarchidae as experimental definitive hosts for <u>Diplostomulum trituri</u>. / Master of Science

Many organisms utilize toxic or noxious compounds as a means of deterring predation. Eastern newts (Notopthalmus viridescens), along with other species in the family Salamandridae, possess a potent neurotoxin called tetrodotoxin (TTX). Although TTX can serve as an effective antipredator mechanism in species of newts with high concentrations (e.g., Taricha), eastern newts have relatively low levels of toxicity in comparison to those species, and it may not serve as an effective antipredator mechanism against all threats. In this case, they may benefit rather by utilizing behavioral changes to avoid initial contact with predators. We tested for predator-avoidance behavior in newts by exposing individuals to kairomones from various predators. We recorded activity patterns of newts when they were exposed to cues from potential predators including bullfrogs (Lithobates catesbeiana), water snakes (Nerodia sipedon) and snapping turtles (Chelydra serpentina), as well as a non-predator (bullfrog tadpoles), and a control (deionized water). Newts reduced activity when exposed to snapping turtle stimuli, but did not change activity when exposed to any other chemical cues. We verified that newts interact with this predator by trapping snapping turtles found in ponds from which newts were collected. Finally, we used turtles caught during this sampling to test whether they are an actual predator of newts and whether newts shift microhabitat use when exposed to this predator. In each replicate, turtles consumed newts, and newts spatially avoided the snapping turtle, relative to a control. The results of these experiments indicate newts rely on predator-avoidance behavior to reduce the probability of being consumed by snapping turtles, but do not reduce activity in response to other potential predators that may only consume them rarely.

Newts have the incredible ability to regenerate many different organs and tissues as adults, including the limbs. Limb regeneration occurs via the dedifferentiation of stump tissue and the formation of a blastema, which provides the majority of cells for the regenerate. Despite all that we have learned about dedifferentiation and blastema formation, the cellular and molecular mechanisms underlying these processes are still poorly understood. We used representational difference analysis (RDA) to identify genes involved in the early dedifferentiation process in newt forelimb regeneration. Our analysis identified approximately 410 unique genes that were differentially regulated during this process. Microarray analysis was used to determine the expression profile of these genes throughout limb and tail regeneration. We used quantitative PCR (qPCR) to validate the expression of a subset of these genes [β-catenin, wntless, dapper, thymosin-β 4 (Tβ4), and thymosin-β 10/15 (Tβ10/15)] in regenerating limb and tail tissue, as well as in differentiating newt myoblasts. We also verified the expression of these genes in the regenerating newt limb using immunohistochemistry (IHC) and in situ hybridization (ISH). Finally, we performed a functional analysis on β-catenin, wntless, dapper, and Tβ4 by overexpressing these genes in mouse myoblasts to examine their effects on differentiation and potential roles in dedifferentiation. Quantitative PCR verified the expression of β-catenin, wntless, dapper, and Tβ4 during limb regeneration and IHC/ISH localized the β-catenin and Tβ4 proteins to the blastema during regeneration. Tβ10/15 was shown by qPCR to be expressed in the tail during regeneration. Overexpression of newt β-catenin, wntless, dapper, and Tβ4 in mouse myoblasts showed that each of these genes has an inhibitory effect on the differentiation of myoblasts into myotubes and, therefore, may play a role in promoting or maintaining the dedifferentiated state. Our work has identified a large number of genes with potential roles in regulating the dedifferentiation process during newt forelimb regeneration. We have also laid a framework from which much more work can be done by drawing on the genes we have identified and the microarray data, which indicate ideal follow-up candidates. Our analysis of specific genes has also increased our understanding of the molecular events occurring during the dedifferentiation process in the regenerating newt limb.