The topic of this master’s thesis is design of infant incubator. The thesis concerns own design of infant incubator that meet the basic technical, ergonomical and social requirements and also brings a new look and shape as solution to the main topic. The infant incubator is designed in regard to modern materials and technologies.

This diploma thesis addresses the issue of prematurely born children. It focuses on the cooperation between neonatologic nurses in perinatal centers and the parents of the newborns. There are 3 goals of this thesis: 1. To explore and describe the level of cooperation and support of the parents and their involvement in the care of the premature newborns in perinatal centers in the Czech Republic. 2. To find out which priorities and expectations of the parents of the premature newborns are. 3. To contribute with these results to the improvement of mutual relationship between a medic and a parent. To achieve the goals described above the methods of quialitative and quiantitative research were used in the empirical part of the thesis. The quantitative research was carried out in perinatal centers in the Czech Republic using questionnaires filled by the nurses who work there. The qualitative research was carried out by questioning the mothers of the premature newborns using individual thematic semi-standardized interview. The results of the research will be provided to all perinatal centers in the Czech Republic that cooperated and can be used to improve the quality of cooperation between neonatologic nurses and parents of premature newborns.

This submitted master's thesis is called "A retrospective view on the neonatal screening". The main aim of this thesis is to map a retrospective view on the neonatal screening in the basis of teoretical fundaments. Thesis deals with theoretical level of problematic. Thesis summarizes the historical development of clinical and laboratory neonatal screening. Next to that there are mentioned procedures executed by a nurse in relation with neonatal screening. Thesis also includes a characteristics of specific illnesses witch can be discovered and then correctly treated. Purpose of this thesis is to broaden a necessary knowledge not only among students and medical staff, but mainly among child nurses witch are performing a blood collections for the neonatal screening and they are in most cases the very first persons to be asked by parents for more informations. Great knowledge of the neonathal screening is elementar for every child nurse. Thesis can also be used as a certain guide for parents which will gain more informations about neonatal screening performed (with parents approval) on their newborn child. This master's thesis have been written after a proper study of available materials and have been summarized to complex text. Scientific analysis, synthesis and explantation were used during work on this thesis. We have used many external sources to create this thesis. Most valuable informations and publications have been found in the internet database PubMed, mostly in english. Very important was research and full text articles provided by the National medical library in Prague. Last but not least were used printed publications in czech and english.

Charles University University of Porto Faculty of Pharmacy in Hradec Králové Faculty of Pharmacy Department of Pharmacology and Toxicology Department of Biological Sciences Student: Karolína Zubatá Supervisor: doc. PharmDr. Martina Čečková, Ph.D. Consultants: Susana Rocha, Ph.D., prof. Alice Santos-Silva, Ph.D. Title of diploma thesis: Oxidative stress biomarkers of the erythrocyte in the newborn - a follow-up study Increased levels of oxidative stress (OS) have been described in healthy, full-term newborns as a consequence of the drastic changes introduced by birth and by the exposure to extrauterine environment. Our intention was to examine the OS levels in red blood cells (RBCs) of neonates and to further understand the changes that the newborn organism undergoes with its newly- acquired autonomy as this knowledge is limited and there are no reference values. Umbilical cord blood samples were collected from a small population of newborns (n = 8) and several hematological and biochemistry parameters were evaluated. Our experimental data consist of OS biomarkers measurements performed in different fractions of blood (RBC membrane, total RBCs and plasma): membrane bound hemoglobin (MBH), lipid peroxidation (LPO), quantification of catalase (CAT) and glutathione peroxidase (GPx) activities,...

Diploma thesis deals with historical formation of neonatal intensive care in the Czech Republic. Its aim is to map the evolution of intensive care, from the very beginning up to the contemporary period, and provide a concise overview of events leading to Czech Republic being one of the countries where level of neonatal care is considered to be most advanced. Theoretical nature of the thesis required a study of historical and contemporary literature, particurarly artticles and books. As an extra supplement, authentic information have been gathered from the pathfinders. In the introduction to the thesis, reason for choosing the topic is described, then current status of knowledge in this area is summarized. Following that, process of creating of the historical analysis is described. Chapter on neonatology introduces and explains terms that are crucial for comprehending the context stated in the historical part of the thesis. Chapter which maps the historical evolution itself is divided into seven sub chapters which describe course of events in the particular periods. Evolution of perinatal care abroad is also shortly described. Thesis reviews an extraordinary evolution of neonatal intensive care which has occurred in very short period of time. During this period pioneers had to overcome many hurdles,...

Women in childbearing age are often affected by autoimmune diseases (AD) associated with the presence of antiphospholipid antibodies (aPL) that may influence further develop-ment of their children. The primary objective of our prospective study was to determine the presence of the following aPL: anti β2 glycoprotein I (anti-β2GPI), anticardiolipin (aCL), antiphosphatidylserine, antiphosphatidylinositol, antihospha- tidylethanolamine, antiphosphatidylglycerol, antiphosphatidic acid, antiannexin V in mothers with defined AD and their children after birth, at 6 and 12 months of life, and to compare the incidence of aPL with a control group. A secondary objective of the study was a 2-year follow-up of children born to aPL negative and aPL positive mothers with AD in order to detect the possible impact of maternal AD on the health of the offspring. In children, we analysed anthropometric data, blood cell count, cerebral and abdominal ultrasound examination, transient evoked otoacoustic emission test (TEOAE), electrocardiograph (ECG), the presence and kinetics of aPL. At the age of 2 years the Bayley Scales of Infant Development (BSID-II) were used for children's assessment of motor, language and cognitive development. 31 mothers from the total examined 82 aPL positive women with AD delivered 34 neonates...

This diploma thesis focuses on assessment of the mother - child relationship during the first three days after childbirth. The theoretical section discusses motherhood, pregnancy and the act of childbirth. From the postpartum standpoint, the mother's psyche and the psychology of the newborn are at the centre of our attention, along with issues related to providing support and ensuring the mother - child bonding processes takes place. Early communication between mother and child is described, as is the origin and development of bonding. To prepare the theoretical section of the thesis, available Czech and foreign sources on the topic were used. The thesis aims to map out how primiparas feel about their new social role as mother, and the nature of their relationship to their newborn in the first days after childbirth. To fulfil this objective, we employ a qualitative-quantitative methodology in the empirical section. Various research methods are used: individual semi-structured interviews, analyses and subsequent data analyses, the "think out loud" method (i.e., the regular verbalisation of one's own thinking process), and the MIRF scale. Ten mothers agreed to take part in the research; all of them in the period from one to three days after the birth of their first child. Based upon an analysis of the data, we determined that in terms of their feelings about motherhood and their relationship with their newborn, these research subjects may be divided into four major groups. The conclusions arrived at in the research were compared with those of Thorstensson et al. (2012), the study that inspired our efforts. Our experience with the interviews carried out with mothers shows that during the semi-structured interview during which they answered our questions these mothers were more focused on themselves, their feelings and issues. However, when they subsequently filled out the MIRF scale, the situation changed. The mothers shifted their focus to their children and their specific feelings towards them. Filling out the MIRF scale improved the mothers' understanding of the individual aspects of their relationship towards their children. The MIRF scale helped them to identify individual areas better. For this reason, we consider the use of the MIRF scale as beneficial from the standpoint of both mothers and the medical staff caring for them and their newborns in the first days after childbirth. The MIRF scale could be utilised practically as part of routine care for mothers and their children. It might improve the support mothers for interacting with their newborns and thereby effectively increase the mothers' sensitivity to their baby's behaviour.

This diploma thesis examines the role of paediatric nurses caring for newborns in the community. It aims to determine if and how newborn care in the home environment takes place and the role of the paediatric nurse. The research is based on one of the primary objectives set out by the member states of the WHO to improve the health of the entire population in the twenty-first century with the focus on community care and its development. The theoretical part is dedicated to the definition of community care and its history in the Czech Republic. We initially focus on how childcare at home was provided in the past. This is followed by a description of the current situation regarding care of a newborn and the mother during the first weeks following birth, both here and abroad. We evaluate the role of the paediatric nurse in terms of nursing care for newborns and research the duties that the nurse carries out within the clinic, family, and community.We have set out two objectives and five research questions. The first objective was to identify nursing care problems encountered by mothers at home and how they solve these problems. The second objective was set out in order to explore how nurses become involved in the community care of a newborn. We have chosen a qualitative method for the research part of the thesis. Data was collected through semi-structured interviews. Subsequently, a qualitative analysis and categorization of collected data was conducted. Two research sample groups were created in the first phase. The first consisted of mothers with newborns, i.e. children from birth to 28 days. The second group consisted of paediatric nurses who work in outpatient care with children and adolescents. The second phase of the research addressed paediatric nurses working in the neonatal clinic, which is run by the hospital's paediatric department. The result of our thesis is the finding that community care provided by the paediatric nurse in the field, in a natural environment and with the cooperation of the entire family and community, as defined, is almost nonexistent. The long-established system of primary care directs all nursing work into the surgeries of the paediatric practitioner, and only the first visit to the newborn, but not in all cases, is conducted at home. This system is insufficient to cover all the specifics of childcare. The needs of the newborns and their parents are becoming more urgent and in the first weeks following the birth the mothers only very slowly begin to gain healthy self-esteem and confidence in their maternal abilities, hence the need for an individual approach and help in the proper care of their baby. They feel there is a lack of information and they do not know how to deal with the needs of their newborn. The most problematic issue described is breastfeeding. The addressed nurses are of the same opinion and also consider breastfeeding as the most common focus of their intervention, and therefore, the most important part of their work.We have discovered that the skills and competencies of the paediatric nurses are sufficient to enable them to become a relevant element in the comprehensive care of newborns within the community. The interventions delivered by the nurse are fully integrated into paediatric nursing.The parents of newborns would, according to our survey, prefer a home visit from health professionals, particularly if it concerns the first contact with the baby and family. The out-patient care does not provide enough time and space to pursue common nursing issues, therefore, home visits are an essential part of the care that should be offered to the mother and baby. Community nurses would help to coordinate the services provided to families as well as help solve the nursing issues that mothers encounter in the first weeks of their child's life. A paediatric nurse would, therefore, become a partner to the parents in a joint effort to ensure the healthy development of their child.

The thesis is focused on nutrition of a preterm infant at an intensive care unit from the point of view of nursing activities. A nurse is faced to problems resulting from prematurity state like insufficient sucking, poor coordination of sucking and swallowing, she enters into interaction with parents who are afraid of the life and subsequently of the future of their beloved child. Natural nutrition, preferably with breast milk is irreplaceable in spite of the high development of initial formulas. This is why the mother of the child, who is vital for the newborn?s nutrition, has to be invited into the team. A hundred per cent enteral nutrition is not always possible, parenteral method has to be applied as well. Provision, evaluation and optimization of infant?s nutrition are results of activities of the whole team, i.e. of a nurse as well. The theoretical part of the thesis contains the findings of trophotherapy of a preterm newborn. It deals with parenteral as well as enteral alimentation, its composition and administration techniques. The empiric part lists and evaluates the research results that led to replies to research questions related to nurse?s contribution to trophotherapy of a preterm newborn, to the influence of permanent nasogastric tube on breathing and mucus congestion of the nose as well as to the awareness of nutrition problems among mothers of preterm infants. Measures resulting from the research results and recommendation for further examination are proposed at the end of the thesis. A brochure Preterm Baby Nutrition designed for distribution among mothers at neonatology and perinatology departments and Recommendations for Gastric Tube Introduction belong to the outputs. We should mention that the latter is based on procedures already presented. It was extended by recommendation of the place of introduction with regard to further intention of enteral nutrition administration.

Abstract Hygiene plays a very important role in our lives. It is often influenced by social, cultural, family and individual factors, as well as by knowledge about health and hygiene. The skin of a child is very soft and sensitive, and therefore requires careful and considerate care throughout the whole period of childhood, especially in the neonatal period and infancy. Skin care of newborns and infants is an essential attribute of the child?s health care. Such care is an individual matter, and it will always depend on family circumstances in which the child lives and on a suitable and proper approach of parents and physicians. The aim of this thesis was to determine the level of knowledge of mothers and the use of such knowledge in skin care of newborns and infants at home. The second objective was to prepare educational material for mothers. The thesis makes use of the questionnaire method. For this purpose, 274 questionnaires were collected. The questionnaire was distributed to mothers of newborns and infants, who take care of their skin at home. The investigation was carried out at pediatrics in the South Bohemian and Pilsen regions. The results of the research are introduced using graphs and some of the results are statistically evaluated by a chi-square test. Two hypotheses were set. Hypothesis 1: Mothers have enough information regarding the skin care of newborns and infants at home. Hypothesis 2: Mothers correctly use the acquired information about skin care of newborns and infants. We were successful in answering both hypotheses in the research study. The analysis and evaluation of the questionnaires shows that mothers are knowledgeable in treating skin of their children in the neonatal period and infancy. However, we managed to prove that some information of the parents is inconsistent with the literature. Therefore an educational material for mothers was created, focusing on skin care of newborns and infants. This educational booklet may be used at pediatric wards.