Invasive measurements of cardiac functioning allows for more accurate measures of cardiac functioning than non-invasive measurements. However, invasive measurements is often not available in clinical settings. By comparing invasive and non-invasive measurements collected in an experimental context, better relationships between non-invasive measurements and cardiac functioning may be found. This master thesis describes the development of two computer applications for simultaneous acquisition, calibration and synchronization of these measurements. The developed applications were tested out during operations on pigs with all measurement sources connected. The results shows that all the desired measurements were successfully acquired by the system. Calibration of the different measurement were also achieved. Different methods for synchronization were tested out during the experiments. It was possible to achieve synchronization of all clocks present in the system. Finally, all of the desired parameters were calculated.