The behaviour of particles in turbulent flow, with emphasis on particle deposition on both the frontside and backside of a cylinder, was investigated by means of Direct Numerical Simulations. One-way coupling between the fluid and the particles was applied. Simulations of turbulence forced at small, intermediate and large scales were run on a three-dimensional domain. The turbulence was used as inlet on a two-dimensional flow domain, where a Lagrangian tracker was used to compute the particle motions. The Reynolds numbers used were Rec = 421 and Rec = 1685. For intermediate Stokes numbers, or particle sizes, the number of particles deposited increased when the Reynolds number was raised. The presence of turbulence lead to a further increase in the deposition for these Stokes numbers, compared to the deposition in laminar flow. The increase was at its highest for large scale forced turbulence. The increased deposition of intermediate Stokes numbers on the frontside of the cylinder in turbulent flow was found to be related to the variance of the effective Stokes number, resulting from the fluctuating nature of the turbulent velocity. The deposition of particles with small Stokes numbers was also altered by the presence of turbulence, but this could not be explained by the variance of Stokes number. The mechanism leading to deposition of these smallest particles is related to the turbulent eddies close to the boundary layer of the cylinder, and will need further study. At the backside of the cylinder, the increased deposition at raised Reynolds number and at the presence of turbulence was related to the magnitude of vorticity. Furthermore, preferential concentration of particles in turbulence was observed in the Rec = 1685 cases, demonstrating the effect of the vorticity magnitude being sustained throughout the domain.