Sediment oxygen demand (SOD) and nutrients releases from the bottom sediments of a water body are important parameters to be included in studies of water quality processes. Two methods to measure SOD, in situ and at laboratory, were applied at Eckie’s Pond on late spring of 2015. Based upon preliminary results, which showed greater values for the in situ method, some modifications of procedures and equipment were made to improve the measurements. Another set of measurements were made on June of 2016, however their results were not conclusive to establish a correlation between these methodologies. As a result of this research, three standard operating procedures (SOP) have been established to measure SOD at shallow waters, first in situ, second at laboratory, and a third SOP to analyze nutrients and metals by using a spectrophotometer.