Subject of this Master´s thesis is analysis and vocal characterization of female roles in the opera Don Giovanni, composed by Wolfgang Amadeus Mozart. I decided to divide this subject into several principal parts. Initially I would like to overview the life and work of the author himself. The next part of this thesis examines the opera as whole; its history of origin, plot issues, characters, performance traditions and main differences between traditional interpretations. As next I would like to address female characters in Don Giovanni opera. Finally this paper presents a deep analysis of the three main female characters of the opera with emphasis on vocal characterization and interpretation of their famous representatives. Finally this paper describes general classification of voice types, discuss the overall problematics of tone and voice production and also evaluate the mechanics and functionality of vocal chords from the physiological point of view.