The conventional methods for diagnosing pathological conditions of the tympanic membrane (TM) and other abnormalities require measuring its motion to an acoustic excitation for its use in a clinical environment. To obtain comprehensive quantitative diagnostic information from the motion of the entire surface of the TM, it is necessary to devise an integrated system capable of accurately recording the motion and induce an acoustic stimulus. To accomplish this goal, a sound presentation system (SPS) capable of impinging acoustic stimulus in the frequency range of 20Hz to 8 kHz at known amplitudes is synthesized in this thesis. This system is then integrated with optoelectronic digital holographic system (OEDHO) which utilizes laser interferometry to record and reconstruct phase shifted images with the help of a digital camera. The OEDHO is capable of accurately recording nanometer scale motion of the TM. The preliminary design of the SPS depends on the physical dimensions of the human ear, such as the diameter of the TM (6-9mm), depth of the ear canal (about 30mm), and also dimensions of the OEDHO system such as: diameter of tip of the otoscope head for optical access (8mm), and possible locations for integration with the OEDHO. The characteristics of the system are based on the intensity of the acoustic stimulus necessary to vibrate the TM (90-110dB SPL), and method of impinging the stimulus. To accomplish this goal, the nature of sound wave propagation through a circular pipe with known dimensions is analyzed analytically, experimentally, and by using finite element analysis (FEA). The pipe is further investigated for optimum parameters using FEA by introducing changes in the diameter (3.8mm, 6mm, 10mm), length of the pipe (30mm, 60mm, 90mm), radius of the curvature (50mm, 75mm, 100mm), and strength of the sound power source (0.2W, 0.4W, 0.6W). The comparative results provide guidelines for the design of the first version of the SPS (SPS_V1). The SPS_V1 consists of a symmetric design to impinge the acoustic stimulus towards the TM and a microphone to measure the sound pressure at the TM. The system is capable of housing a range of speakers from 2mm to 15mm in diameter. The SPS_V1 can directly interface with the standard medical speculums used for human ear testing. Also, the system is capable of interfacing with all available versions of the OEDHO. The SPS_V1 is currently being evaluated in a medical-research environment to address basic otological questions regarding TM function. The performance characterization of the system inside an artificial ear canal with two different speaker configurations is herein shown, and the potential improvements and utilization are discussed

The conventional methods for diagnosing pathological conditions of the tympanic membrane (TM) and other abnormalities require measuring its motion while responding to acoustic excitation. Current methodologies for characterizing the motion of the TM are usually limited to either average acoustic estimates (admittance or reflectance) or single-point mobility measurements, neither of which is sufficient to characterize the detailed mechanical response of the TM to sound. Furthermore, while acoustic and single-point measurements are useful for the diagnosis of some middle ear disorders, they are not useful in others. Measurements of the motion of the entire TM surface can provide more information than these other techniques and may be superior for the diagnosis of pathology. In this Thesis, the development of an optoelectronic holographic otoscope (OEHO) system for characterization of nanometer scale motions in TMs is presented. The OEHO system can provide full-field-of-view information of the sound-induced displacements of the entire surface of the TM at video rates, allowing rapid quantitative analysis of the mechanical response of normal or pathological TMs. Preliminary measurements of TM motion in cadaveric animals helped constrain the optical design parameters for the OEHO, including the following: image contrast, resolution, depth of field (DOF), laser power, working distance between the interferometer and TM, magnification, and field of view (FOV). Specialized imaging software was used in selecting and synthesizing the various components. Several prototypes were constructed and characterized. The present configuration has a resolution of 57.0 line pairs/mm, DOF of 5 mm, FOV of 10 ´ 10 mm2, and a 473 nm laser with illumination power of 15 mW. The OEHO system includes a computer controlled digital camera, a fiber optic subsystem for transmission and modulation of laser light, and an optomechanical system for illumination and observation of the TM. The OEHO system is capable of operating in two modes. A 'time-averaged' mode, processed at video rates, was used to characterize the frequency dependence of TM displacements as tone frequency was swept from 500 Hz to 25 kHz. A 'double-exposure' mode was used at selected frequencies to measure, in full-field-of-view, displacements of the TM surface with nanometer resolution. The OEHO system has been designed, fabricated, and evaluated, and is currently being evaluated in a medical-research environment to address basic science questions regarding TM function. Representative time-averaged holographic and stroboscopic interferometry results in post-mortem and live samples are herein shown, and the potential utilization discussed.