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Ovarian differentiation in an ancient vertebrate: timing, candidate gene expression, and global gene expression in parasitic and non-parasitic lampreysSpice, Erin 22 August 2013 (has links)
Lamprey adults may be parasitic or non-parasitic, but the genetic basis of life history type is unknown. Although external differences between types are not apparent until metamorphosis, previous studies have suggested histological differences during ovarian differentiation. This study examined potential differences between parasitic chestnut lamprey Ichthyomyzon castaneus and non-parasitic northern brook lamprey I. fossor before, during, and after ovarian differentiation, using histological examination of the gonad, quantitative reverse-transcriptase PCR, and RNA sequencing. There were no observable differences in the timing or nature of ovarian differentiation. However, there was evidence of differential expression of individual genes associated with growth, apoptosis, and fecundity and of sets of genes associated with energy and lipid metabolism. The sequence resources developed in this project will be useful for future examination of the genetic basis of lamprey life history type and of the genes controlling sex differentiation in these ancient vertebrates.
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Ovarian differentiation in an ancient vertebrate: timing, candidate gene expression, and global gene expression in parasitic and non-parasitic lampreysSpice, Erin 22 August 2013 (has links)
Lamprey adults may be parasitic or non-parasitic, but the genetic basis of life history type is unknown. Although external differences between types are not apparent until metamorphosis, previous studies have suggested histological differences during ovarian differentiation. This study examined potential differences between parasitic chestnut lamprey Ichthyomyzon castaneus and non-parasitic northern brook lamprey I. fossor before, during, and after ovarian differentiation, using histological examination of the gonad, quantitative reverse-transcriptase PCR, and RNA sequencing. There were no observable differences in the timing or nature of ovarian differentiation. However, there was evidence of differential expression of individual genes associated with growth, apoptosis, and fecundity and of sets of genes associated with energy and lipid metabolism. The sequence resources developed in this project will be useful for future examination of the genetic basis of lamprey life history type and of the genes controlling sex differentiation in these ancient vertebrates.
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Cibles et voies de signalisation régulées par FOXL2 au cours de la morphogenèse ovarienne / Target Genes and Signaling Pathways Regulated by FOXL2 During Ovarian DifferentiationEl Zaiat, Maëva 16 October 2015 (has links)
FOXL2 est un facteur de transcription crucial pour la fonction ovarienne. Dans l'espèce humaine, des mutations hétérozygotes de ce gène sont responsables de la survenue d'un syndrome associant des malformations des paupières à une insuffisance ovarienne prématurée. De même chez la souris, l'invalidation totale de Foxl2 conduit à un blocage de la folliculogenèse et donc à une infertilité femelle. Chez la chèvre, la mutation Polled Intersex Syndrome (PIS) engendre le silence transcriptionnel de FOXL2 dans les gonades XX PIS-/- ce qui conduit à une inversion sexuelle et à la différenciation de testicules à la place d'ovaires chez les animaux génétiquement femelles homozygotes pour la mutation (inversion sexuelle de type mâle XX). FOXL2 est donc déterminant pour la différenciation ovarienne très précocement au cours du développement dans l'espèce caprine, alors qu'il ne semble impliqué que plus tardivement dans l'établissement de la fertilité chez la souris et la femme. Afin de comprendre ces différences entre espèces, nous avons recherché quels étaient les gènes et les voies de signalisation régulés par FOXL2 dans l'ovaire de chèvre au début de sa différenciation. Grâce à un séquençage à haut-débit des transcrits présents dans trois types de gonades caprines (testicules XY, ovaires XX et gonades XX PIS-/- (qui n'expriment pas FOXL2)) au début de leur différenciation, nous avons pu (i) mieux caractériser le rôle de FOXL2 dans l'ovaire caprin et montrer qu'il y agit avant tout comme un facteur anti-testiculaire, et (ii) mettre en évidence de nouveaux gènes pro-ovariens comme DMXL2 et étudier son rôle putatif dans la fonction ovarienne grâce à des expériences fonctionnelles chez la souris. / FOXL2 is a transcription factor which is crucial for the ovary. In humans, heterozygous mutations are responsible for the BPES syndrome characterized by eyelid anomalies and premature ovarian failure. Similarly in mice, Foxl2 invalidation leads to complete folliculogenesis disruption and female infertility. In the goat, the Polled Intersex Syndrome mutation is responsible for the transcriptional silencing of FOXL2 in XX PIS-/- gonads that leads to female-to-male sex reversal and the differentiation of testes instead of ovaries in genetically female animals homozygous for the mutation. Thus, FOXL2 is determining for ovarian differentiation early during development in goats, whereas it is involved in fertility tardily in mice and women. In order to understand these species-specific differences, we searched for the genes and pathways regulated by FOXL2 in early goat ovaries. Thanks to RNA-sequencing of goat XY testes, XX ovaries and XX PIS-/- gonads (lacking FOXL2) at the beginning of their differentiation, we were able to (i) better characterize the role of FOXL2 in goat ovaries and show that it acts mainly as an anti-testis factor, and (ii) highlight new pro-ovarian genes like DMXL2, and study its putative role during ovarian development using functional experiments in the mouse.
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Transcriptomic analysis of ovarian development in parasitic Ichthyomyzon castaneus (chestnut lamprey) and non-parasitic Ichthyomyzon fossor (northern brook lamprey)AJMANI, NISHA 31 March 2017 (has links)
Lampreys are primitive jawless fishes that diverged over 550 million years ago. As adults, they are either parasitic or non-parasitic. In non-parasitic species, sexual differentiation and oocyte development generally occur earlier than in parasitic species; fecundity is reduced and sexual maturation is accelerated following metamorphosis. The genes controlling ovarian differentiation and maturation in lampreys are poorly understood. This study used RNA-Seq data in the parasitic chestnut lamprey Ichthyomyzon castaneus and non-parasitic northern brook lamprey Ichthyomyzon fossor to identify suites of genes expressed during different stages of ovarian development that show different developmental trajectories with respect to ovarian differentiation and sexual maturation. For this, reference-guided and de novo assembly pipelines were designed for studying a non-model species. To test and explore the relative advantages of the pipelines, expression of insulin superfamily genes was used. This research helps to identify genes involved in lamprey ovarian development and provides insight into evolution of the insulin superfamily in vertebrates. / May 2017
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