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Diploma thesis is aimed at evaluation of soil organic carbon dynamic and humic substances quality in monoculture of spring barley and Norfolk crop rotation system. Further we followed the influence of different soil tillage system (minimalization and conventional tillage) on to humus and humic substances dynamic. Gleiyc Fluvisol was classified and observed during 2017 – 2018 within field long term experiments of Mendel University in Brno at locality Žabčice. Measured data set was evaluated using program StatisticaCZ12, software (StatSoft software Inc., Tulsa, Oklahoma, USA). Obtained results shown that statistically significant differences between Norfolk and monoculture were in following parameters: organic carbon content, humus content and humic substances content. Higher values were in Norfolk crop rotation system.

The subject of this research is a level of aberration of soil on selected sites in the land register of Vlkov u Osové Bítýšky village. During a Complex soil survey (1967/1968) were collected three samples In 1966 and 1967, in this territory. From these samples were selected data from texture analysis pH and humus. The old soil sampling sites were re-localized and from the very spots, new samples were taken from the depth of approximately 75 cm in order to gain current results. These new samples were analyzed in a laboratory and these results were compared with the 50 years old ones. This allowed us to evaluate changes in soil on the selected sites.