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The dissertation thesis consists of four papers that have been published in scientific journals. Study n. 1 The need to recover evidence from water is quite common in criminal investigation. The article deals with the possibility to collect human scent from such objects and with the ability of specially trained dogs to match such scent samples with scent samples collected from detained suspects. During an experiment, designed as a blind one, it has been proved, that human scent can survive on submerged objects and can be later used for scent identification. Study n. 2 To collect odors the Czech Republic Police use special fabric sorbents manufactured under the registered mark ARATEX. Before use the fabric sorbent is treated by water vapor sterilization. After the scent identification the sorbent is destroyed. The goal of the study was to verify if the vapor sterilization is effective enough to remove human scent that has already penetrated into the sorbent structure or in other words if the sorbent can be exposed to vapor sterilization and then used again. Specially trained dogs were used to match starting odors with target odors in the line-ups. The results showed that dogs are able to correctly match human odors even after they have being exposed to vapor sterilization. Study n. 3 The purpose of the study was to determine the temperature at which the human scent is degraded so that a dog would not be able to identify it. In contrast to expectations, eight dogs used in the experiment almost flawlessly identified human scents from five scent donors exposed to temperatures of 100°C, 200°C, 300°C, 400°C, 500°C, 600°C, 700°C, and 800°C. Only two of the dogs were able to identify 5 of 15 scent samples exposed to 900°C. No dog identified a scent exposed to 1000°C. Our study verified heat survivability of human scent far beyond existing expectations. Study n. 4 We tested the hypothesis that if odor fallout (the release of a human odor onto an untouched object) in human subjects exists, then holding a hand above an absorbent will produce a detectable scent which will be subsequently matched in a detection test by trained dogs. Scents were collected from seven males to sterile cotton absorbent squares. The left hand was used to get the control scent and the right hand served as the target scent. Each experimental subject was sitting and his left hand was laid down on a cotton square for 3 min. The right hand was held 5 cm above another cotton square for 3 min. The scent identification was done by two specially trained police German shepherds. Both dogs performed 14 line-ups and correctly matched the collected scents of all test subjects. The results suggest the existence of human odor fallout, whereby a human scent trace is left by humans even if they do not touch an object.

In the recherche part of our thesis we familiarize ourselves with the human odor, theories of its origin, definition, anatomy of human skin and odors, composition of individual human odors and with odor secretion. We also look closer on the topics of odor absorbent and its use in the world, the transmission of odors to odor sensors and securing of scents. This section of our thesis discusses the resistance and survival abilities of odor, scent identification method and its history, organic acids, and defines the application of laundry detergents and ultrasonic washers. Aim of our thesis was to verify the relevance of the use of chemical detergents in the purification process when working with scents. We tried to verify the ability of detergents to degrade the human scent on odor absorbents to such an extent, that specially trained dogs would not be able to identify it. Our experiment was following a precise determination methodology. In the first phase samples were collected from the hands of targeted persons to a scent carrier - a metal cylinder (extirpate odour), by one researcher. In the second phase samples were collected from the body to a textile carrier, by another researcher. The metal scent carriers were processed. Some of them were left as they were (control sample) and some were exposed to chemical detergents with and without usage of an ultrasonic washer. In the experiment where we tried to identify the odour samples, six bitches of German shepherd were used. They were specially trained for odour identification. Each dog was let three times to identify the target scent exposed to a detergent and three times to identify the target scent not exposed to a detergent. Target smell was randomly deposited between other samples and its position was changed, so that the handler did not know its position and thus was unable to affect the work of his dog. The indicator of positive identification was a sign the dogs were taught - to sit or lay in front of a sample. Any dog was unable to identify the scent which was exposed to chemical detergent and all dogs identify the scent unexposed to a chemical detergent. Our experiment has shown that the use of chemical detergents in the purification process when working with scents is relevant to the degradation of individual human scent.

Survivability of human scent was observed by many scientists mainly in artificial conditions (Hudson et al., 2009; Santariová et al. 2012; Preti et al., 2006). Against this exists a few researches about survivability of human scent in nature conditions (King et al., 1964; Santariová et al., 2016). On glass tubes were taken individual humant scent from 6 volunteers. Target scents were left outdoor during 2 different periods of year. Winter samples from 3 target people were outdoor from November to May and summer samples from other 3 people from May to December. Always after 3, 4, 5 a 6 months was taken scent from 1 glass tube of each target person. After last time period was taken scent from target people on cotton textile. Scent of other volunteer was taken at the same time for purpose scent deceptions. Then was observed if dogs will be able to identify these samples corectly. Specially trained dogs on scent identification line-ups get sample from outdoor environment and they should choose the identical human scent from line of 6 samples (with 1 target and 5 deceptions). The experiment showed, that dogs are able to identify winter samples in all time periods but are not able to identify summer samples in whatever time period. Time periods do not show any diference. The biggest influence on quality change of individual human scent has intensity of sun radiation.

The aim of this thesis was to make the evalution of the produced emissions of odorours substances and dust particles of PM 2,5 fraction from intensive poultry farming. Measurements were implemented according to the valid methodologies in poulty farm which is in Sudoměřice u Bechyně.

As agricultural production grows, the focus is on improving air quality, which affects not only residents around farms, but also climate change on a global scale. This thesis deals with the measurement of particulate pollutants and odor emissions in intensive poultry farming. The measurements were carried out at the Farma U Lesa in Sudoměřice u Bechyně in the spring and autumn of 2018. The production specific emission of odorous substances into the air was evaluated using dynamic olfactometry according to ČSN EN 13 725, which ranged from 0.01 to 0 according to the fattening cycle phase, 3 OUE. Ks-1. s-1. Compared to the BREF reference document, which lists emission odor values from 0.032 to 0.7 OUE. Ks-1. s-1 the measured values on the farm Mr. Šonky came out very low. Dust particles of PM10 fraction were measured according to the ČSN EN 12 341 methodology for the measurement of dust particles and the production specific emission of PM10 particles was found, which during the whole fattening cycle was measured to a maximum of 0.0025 kg. Ks-1.year-1. The measurement results were compared with the BREF reference document, where the maximum permissible production of solid pollutants of this fraction from 0.004 to 0.025 kg. Ks-1.year-1. In comparing the measured dust emission values with the BREF, the production of harmful emissions was very low.

Environmental pollution, natural resources consumption and global climate change have been threats for mankind since 21 century. In order to antedate environmental pollution in many countries of the world including The Czech Republic we use legislative tool as a process of influence of the environment EIA. Substitution of non-renewable energy sources for renewable ones may retard global climate change markedly. This thesis deals with analysis of specific biogas plants located in Moravian-Silesian region in relation to the environment and population. It means that it deals with biomass utilization as a renewable source of energy. Within EIA process, it assesses the unity rate between prediction, said in documentation, and the real state after implementation. As far as the population perspective is concerned, poeple assess great strength, weaknesses, opportunities and threats. Results of the research showed different perceptions of biogas before and after project implementation. The risk of discomfort housing (smell, increased traffic congestion, noise) threatens residents in older facilities where an EIA was not yet part of the legislation. The results also showed compliance with the information provided in the EIA documentation in relation to the population and the environment, and the state after realizing the plan of a biogas plant. The thesis brings us new information within post-project analysis, it shows us the importance of EIA process and publicity involvement into this process. Defining the specific effects of biogas plants by mutual cooperation between the investor and the public authorities can be achieved some functioning device with regard to population and environmental components.

This thesis is focused on wireless sensor networks and implementation tools for creation and simulation of applications. It describes WSnageNt project, which is intended to support agents in wireless sensor networks. The main objective of this thesis is to extend the WSageNt project with new features that will allow to create agents with more capabilities. These features will be then used to extend the WSageNt project with network node movement tracking capability.

Dorso-abdominal scent glands (= DAGs) of larvae represent one of apomorphic characters of insects order Heteroptera. These glands can persist until adults in different taxa. The persistence of DAGs were proved in the members of the family Acanthosomatidae. The ontogenetic development of DAGs cuticular structures were studied in all stadia, from 1st larval instar to adults of acanthosomatid Elasmucha ferrugata (Fabricius, 1787). The study concerned external structures and sculptures associated with DAG ostiole and areas of their surfaces, as well as internal structures, shape of gland reservoir and number of conducting ductules of proper glandular units. The light microscope and stereomicroscope and scanning electron microscope were used for this comprehensive study.

Existence of larval dorsoabdominal scent glands (DAGs) together with occurence of adult metathoracic scent glands is one of the important autapomorphic characters of taxon Heteroptera within taxon Insecta. DAGs can persist until adults in some species of this taxon. The persistence of DAGs were proved also in members of infraorder Pentatomomorpha. Targets of this thesis are: collect the knowledge about persistent adult DAGs in selected representative members of important families of taxon Pentatomomorpha; study and compare the condition of the DAGs in the oldest larval instars and adults; clarify the significance of existence the openings of conducting ductules of proper glandular units in reservoir intima of DAGs in study of the persistence of these glands. For study were used stereomicroscope, light and scanning electron microscope. Key words: Pentatomomorpha, Heteroptera, dorsoabdominal scent glands, openings of conducting ductules of glandular units, larvae of 5th instar, persistence in adults, persistence patterns, light microscope, scanning electron microscope