Thesis (D.M.A.)--University of Nebraska-Lincoln, 2010. / Title from title screen (site viewed July 8, 2010). PDF text: xi, 139 p. : music ; 4 Mb. UMI publication number: AAT 3398463 . Includes bibliographical references. Also available in microfilm and microfiche formats.

Klebsiella oxytoca SYSU-011 isolated from wastewater of a metal-plating plant in southern Taiwan had been proven to be able to degrade cyanide. In this study, we performed proteomic analyses to understand the mechanism of tetracyanonickelate (TCN) resistance in K. oxytoca by using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and MALDI-TOF-MS techniques. There were 91 protein spots had been induced or overexpressed (≥2 fold) by TCN. Among them, 44 protein spots were successfully identified by MALDI-TOF-MS. The expressed proteins that had been escalated including chaperone, glutathione S-transferase (GST) and alkyl hydroperoxide reductase (AHR) were involved in the TCN detoxification process. Fructose-bisphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglucomutase and 6-phosphogluconolactonase were involved in energy-producing process; nitrogenase and glutamine synthetase (GS) were required to regulate nitrogen assimilation. We also analyzed the K. oxytoca membrane proteins. Twenty six proteins spots had been successfully identified by MALDI-TOF-MS (out of 41 protein) that were induced ≥2 fold by TCN. These proteins induced RND-drive efflux proteins (RND family, MFP subunit, outer membrane factor A and outer membrane factor TolC) and cation efflux system. These efflux pumps could transport nickel ion out of the cells. The induced ATP-binding cassette (ABC) proteins may also play a role in transportation of metal-cyano complexes TCN and nutrition. By this study, we had a better understanding on the defense mechanism of K. oxytoca after exposure to TCN.

Thesis (M.A.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves [281]-285).

La compréhension des mécanismes cellulaires et moléculaires qui sont mis en jeu au moment de la croissance et la maturation pré-ovulatoire induite par la LH, permettra de définir des marqueurs de qualité et de maturité du follicule destiné à ovuler, et ainsi de mieux anticiper le moment de l’ovulation. L’objectif majeur de cette thèse était d’identifier certain des facteurs régulateur impliqués dans la maturation folliculaire par deux approches globales d’analyse protéomique et transcriptomique. La première étude a permis d’établir pour la première fois les cartes protéiques du liquide folliculaire équin et canin. Les analyses comparatives des liquides folliculaires provenant de différents stades physiologiques n’ont montré, dans nos conditions expérimentales, que peu de différences. Nos résultats obtenus dans la deuxième étude indiquent que les différentes méthodes enrichissement du liquide folliculaire peuvent améliorer, pour certaines de manière conséquente, la résolution des gels 2D-PAGE. Notre étude transcriptomique globale a révélé un groupe de gènes différentiellement exprimés dans les cellules folliculaires aux différents stades étudiés. Ces gènes sont potentiellement impliqués pendant le développement folliculaire dans l’espèce équine. Les deux approches (protéomique et transcriptomique) que nous avons utilisées au cours de ce travail sont complémentaires car la connaissance des gènes exprimés par les cellules folliculaires peuvent permettre d’identifier certains gènes codant pour des protéines sécrétoires retrouvées dans le liquide folliculaire. / An understanding of the cellular and molecular mechanisms involved in the growth and maturation of the preovulatory follicle induced by LH, will help us to understand and identify the markers of quality and maturity of the follicle destined to ovulate, and better anticipate the time of ovulation. The main objective of this thesis was to identify some regulatory factors involved in follicle maturation using two global approaches: proteomic and transcriptomic analysis. The first study established for the first time the protein map of equine and canine follicular fluids. The comparative analyses of follicular fluid from different physiological stages were shown little or no difference in our experimental conditions. Results obtained with the second study suggested that between depletion and enrichement methods, the enriched follicular fluid can improve for some consistent manner, the resolution of 2D-PAGE gels. Our global transcriptomic study revealed a group of genes differentially expressed in follicular cells at different physiological stages. These genes are potentially involved during follicle development in the equine species. The two approaches (proteomic and transcriptomic) that we used in this work are complementary, as the knowledge of genes expressed by follicle cells can help to identify some genes coding for secretory proteins secreted in follicular fluid.

Patricia K. Page's prose and poetry exhibit a dynamic creative tension resulting from the conflict of opposites in theme and imagery patterns and in the poet's attitude and perception of her subjects. The concept of separateness results from the thematic opposition of forces of solitude and multitude which focus on the despair of the isolated individual unable to emerge from his 'frozen' cave-like existence and to attain a community of shared feeling. Highly developed black-white dichotomy of images reinforces the conflict of obsessive self-love and pity with the universal need for self-awareness. Some of the poet's subjects succeed in this human search for truth, beauty, and self-fulfillment. Many, however, succumb to loneliness and paranoic isolation. The basic conflict is seen through surface-depth alternations of imagery, the phases of the Rebirth archetypal pattern of transition from terrestrial to aquatic form. The conflict of opposites of isolation and involvement in her early work emphasizes the strength of the pull towards confinement of the self. Then, as Page progresses in objective perception of her individuals, there is a loosening of the force of isolation and a gradual emergence of the individual from solitude into multitude. The conflict of opposites of restraint against freedom grows out of the basic juxtaposition of forces. Self-isolation and the vulnerability of innocence are linked as states of unawareness. Children, social classes and adult individuals are portrayed as victims of indifferent constraining authority, social barriers, and war. Page's Marxian love of humanity dissolves into a Freudian interpretation of communal existence as an escape from the fears of solitude. The poet's presentation of an active social consciousness is a continuance of the contra-positioning of freedom and restrain operating on and within individuals. In Page's work, organic imagery is suggestive of the powers of vitality and the life force - of multitude, universality, and personal freedom. Stagnation, inertia, isolation, and self-love are visualized by images of metal, stone or rock. This metal-flower contraposition evolves into archetypal antipathies of Paradise and Hades. The descent from sunlit gardens above to the dark caverns below takes the form of Freud's Nirvana principle or the first phase of the Rebirth cycle. And the renewal of vitality, the modulation from stone into flower marks the final ascent stage of Rebirth. The pattern of Rebirth related to man's rituals has symbolic meaning for Page's work. The goal of the poet is to remove the "filter of subjectivity" placed over reality and to realize the potential of being reborn into awareness. Ararat becomes a symbol of the power of regeneration and of the unity of all life. P. K. Page presents conflict of opposites in themes and imagery. Yet her primary concern remains that the need for communal experience is greater than the negative desire for self-isolation from reality; that love is more sustaining than pity; that freedom is preferable to indifferent authority; that the search for beauty and truth must overcome fear and horror in the lives of individuals. / Arts, Faculty of / English, Department of / Graduate

Current scholarship on Shakespeare’s The Merry Wives of Windsor mostly focuses on topics surrounding Sir John Falstaff’s presence, mythical allusions, and the questionable date of publication. Although their actions are the driving force of the play, the woman frontrunners, Mistress Ford and Mistress Page, receive little scholarship attention. Anne Page, daughter of Mistress Page and wife of Fenton, also dictates the course of the play by taking control of her betrothal. Yet she remains underappreciated in scholarship. This thesis highlights important characteristics of all three wives of Windsor in addition to justifying Anne Page’s role as a wife of Windsor. Through close readings of the text and analyses of scholarship immediately relevant to these wives and common attributes of women in Renaissance England and Shakespeare plays, I argue these strong, multifaceted women refute stereotypical female roles and undermine patriarchal authority aligning themselves with other idolized outspoken woman characters in Shakespeare plays.

As diarréias neonatais constituem-se em um dos mais importantes fatores econômico e sanitário nas granjas suínas, quer pela mortalidade, quer pelas perdas agregadas ao atraso no desenvolvimento dos leitões, à profilaxia e ao manejo. Os rotavírus ocupam lugar de destaque pela rápida disseminação dentro do plantel, bem como pelo potencial zoonótico, dada a probabilidade de rearranjo ou recombinação genética entre amostras humanas e animais O objetivo deste trabalho foi detectar a presença de rotavírus a partir de 277 amostras fecais de leitões com quadro clínico de diarréia, provenientes dos Estados do Rio Grande do Sul (RS), Santa Catarina (SC), Paraná (PR), Mato Grosso do Sul (MS), Mato Grosso (MT), São Paulo (SP), Rio de Janeiro (RJ) e Minas Gerais (MG) entre os anos de 2009 e 2011 e analisar o perfil eletroforético de migração dos segmentos genômicos bem como diferenças de eletroferótipos nas amostras positivas, pela técnica de eletroforese em gel de poliacrilamida (PAGE). Das 277 amostras fecais diarréicas de leitões analisadas, 25 foram positivas (25/277= 9%). De conformidade com os Estados de origem, foram verificadas as frequências de 20% (1/5) no RS, 11,1% (1/9) em SC, 12,5% (1/8) no PR, 15,3% (6/39) em MS, 14,2% (3/21) em MT, 6,7% (5/74) em SP, 0% (0/7) no RJ e 7% (8/114) em MG. Pela análise da migração eletroforética dos segmentos genômicos, todas as 25 amostras positivas apresentaram perfil eletroforético compatível com o RV-A, tal como a amostra padrão NCDV com migração característica em quatro classes ou agregados [4-2-3- 2]. Foram observadas pequenas diferenças na velocidade de migração de um ou mais segmentos dentro da mesma classe. Estes resultados evidenciam a importância da PAGE como metodologia de diagnóstico e de investigações epidemiológicas nas rotaviroses suínas. / The neonatal diarrhea constitute one of the most important economic and health factors in pig farms either by death or by the aggregate losses to the delay in the development of piglets, prophylaxis and management. Rotaviruses have a prominent role by the rapid spread within the herd, as well as the zoonotic potential, given the likelihood of genetic reassortment or recombination between human and animal samples. The objective of this study was to detect the presence of rotavirus from fecal samples from 277 piglets with clinical cases of diarrhea, from the states of Rio Grande do Sul (RS), Santa Catarina (SC), Paraná (PR), Mato Grosso do Sul (MS), Mato Grosso (MT), São Paulo (SP), Rio de Janeiro (RJ) e Minas Gerais (MG) between the years 2009 and 2011 and to analyze the electrophoretic migration of genomic segments and electropherotype differences in positive samples, by polyacrylamide gel electrophoresis (PAGE) technique. From 277 piglets diarrheal stool samples analyzed, 25 were positive (25/277 = 9%). In accordance with the States of origin, were observed frequencies of 20% (1/5) in the RS, 11.1% (1/9) in SC, 12.5% (1/8) in PR, 15.3 % (6/39) in MS, 14.2% (3/21) in MT, 6.7% (5/74) in SP, 0% (0/7) in Rio de Janeiro and 7% (8/114) in MG. For the analysis of the electrophoretic migration of genome segments, all 25 positive samples showed electrophoretic profile compatible with the RV-A as a standard sample NCDV, showing characteristic fourth class or aggregates [4-2-3-2]. We observed small differences in the migration speed of one or more segments within the same class. These results highlight the importance of PAGE as a method of diagnosis and epidemiological investigations in the porcine rotavirus.

The extraordinary growth in both the size and popularity of the World Wide Web has generated a growing interest in the identification of Web page genres, and in the use of these genres to classify Web pages. Web page genre classification is a potentially powerful tool for filtering the results of online searches. Although most information retrieval searches are topic-based, users are typically looking for a specific type of information with regard to a particular query, and genre can provide a complementary dimension along which to categorize Web pages. Web page genre classification could also aid in the automated summarization and indexing of Web pages, and in improving the automatic extraction of metadata. The hypothesis of this thesis is that a byte n-gram representation of a Web page can be used effectively to classify the Web page by its genre(s). The goal of this thesis was to develop an approach to the problem of Web page genre classification that is effective not only on balanced, single-label corpora, but also on unbalanced and multi-label corpora, which better represent a real world environment. This thesis research develops n-gram representations for Web pages and Web page genres, and based on these representations, a new approach to the classification of Web pages by genre is developed. The research includes an exhaustive examination of the questions associated with developing the new classification model, including the length, number, and type of the n-grams with which each Web page and Web page genre is represented, the method of computing the distance (dissimilarity) between two n-gram representations, and the feature selection method with which to choose these n-grams. The effect of preprocessing the data is also studied. Techniques for setting genre thresholds in order to allow a Web page to belong to more than one genre, or to no genre at all are also investigated, and a comparison of the classification performance of the new classification model with that of the popular support vector machine approach is made. Experiments are also conducted on highly unbalanced corpora, both with and without the inclusion of noise Web pages.

The extraordinary growth in both the size and popularity of the World Wide Web has generated a growing interest in the identification of Web page genres, and in the use of these genres to classify Web pages. Web page genre classification is a potentially powerful tool for filtering the results of online searches. Although most information retrieval searches are topic-based, users are typically looking for a specific type of information with regard to a particular query, and genre can provide a complementary dimension along which to categorize Web pages. Web page genre classification could also aid in the automated summarization and indexing of Web pages, and in improving the automatic extraction of metadata. The hypothesis of this thesis is that a byte n-gram representation of a Web page can be used effectively to classify the Web page by its genre(s). The goal of this thesis was to develop an approach to the problem of Web page genre classification that is effective not only on balanced, single-label corpora, but also on unbalanced and multi-label corpora, which better represent a real world environment. This thesis research develops n-gram representations for Web pages and Web page genres, and based on these representations, a new approach to the classification of Web pages by genre is developed. The research includes an exhaustive examination of the questions associated with developing the new classification model, including the length, number, and type of the n-grams with which each Web page and Web page genre is represented, the method of computing the distance (dissimilarity) between two n-gram representations, and the feature selection method with which to choose these n-grams. The effect of preprocessing the data is also studied. Techniques for setting genre thresholds in order to allow a Web page to belong to more than one genre, or to no genre at all are also investigated, and a comparison of the classification performance of the new classification model with that of the popular support vector machine approach is made. Experiments are also conducted on highly unbalanced corpora, both with and without the inclusion of noise Web pages.

A significant fraction of today's Internet traffic is associated with popular web sites such as YouTube, Netflix or Facebook. In recent years, major Internet websites have become more complex as they incorporate a larger number and more diverse types of objects (e.g. video, audio, code) along with more elaborate ways from multiple servers. These not only affect the loading time of pages but also determine the pattern of resulting traffic on the Internet. In this thesis, we characterize the complexity of major Internet websites through large-scale measurement and analysis. We identify thousands of the most popular Internet websites from multiple locations and characterize their complexities. We examine the effect of the relative popularity ranking and business type of the complexity of websites. Finally we compare and contrast our results with a similar study conducted 4 years earlier and report on the observed changes in different aspects.