Thesis--PlanB (M.S.)--University of Wisconsin--Stout, 2003. / Includes bibliographical references.

For individuals diagnosed with a glioma, survival rates have shown little improvement over the last 40 years due to the heterogeneity of tumours and the difficulty of specifically targeting the tumour whilst sparing surrounding healthy tissue. Altered gene methylation is often seen in glioma cells, but methylating agents such as folate, may reverse aberrant methylation. Folate treatment has shown a beneficial effect, reducing risk of certain cancers (colorectal, breast, squamous cell carcinoma) but other studies have shown detrimental results whereby proliferation of cancer increased (mammary, prostate). The aim of this thesis was to investigate the opposing roles of folate in glioma. The glioma cell lines 1321N1, U87 MG and non-cancerous glial SVGp12 cells were used for analysis. Cells were grown in folate deficient, folic or folinic acid supplemented media and compared to standard cell culture media. Cell viability, cell cycle and apoptosis analysis along with methylation status and protein expression of the genes of interest; PTEN, FOLR1, RFC, PCFT, and MTHFR were analysed to determine differences between cell lines following treatment. The investigation showed that folic and folinic acid behaved differently depending on concentration used and the cell lines treated. Folic acid at 5 µg/ml significantly increased cell viability and protein expression levels in the U87 MG and SVGp12 cell lines, whilst the folinic acid (35 µg/ml) resulted in significant decreased cell viability, increased apoptotic activity and down regulation of the folate transporters in the 1321N1, U87 MG and SVGp12 cell lines. Folate treatment did not significantly alter cell cycle phase. Altered methylation of genes specific for folate metabolism and transport did not explain the cytotoxic effects of folate in cell lines. In conclusion, the work presented here signifies that folinic acid rather than folic acid would be more suitable for glioma treatment. The effect of folinic acid treatment on glioma had not been previously studied, and the knowledge obtained here regarding the effects of folic and folinic acid treatment on folate transporter expression in glioma has advanced understanding.

Thesis--PlanB (M.S.)--University of Wisconsin--Stout, 2003. / Includes bibliographical references.

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Thesis (Ed.D.)--University of West Florida, 2009. / Title from title page of source document. Document formatted into pages; contains 157 pages. Includes bibliographical references.

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Thesis (Ed.D.) -- Teachers College, Columbia University, 1991. / Typescript; issued also on microfilm. Sponsor: Victoria Marsick. Dissertation Committee: William Yakowitz. Includes bibliographical references: (leaves 205-223).

Thesis (Masters Diploma (Medical Technology))-- Cape Peninsula University of Technology, 1992 / Studies that document the higher incidence of tuberculosis as well as the variable efficacy of the BCG vaccine in Black, compared to White, populations have alluded to resistance or susceptibility to tuberculosis being genetically controlled.The HIA system has been associated with many diseases involving an immune aetiology. It has been shown that T cell receptor genes have limited restriction fragment length polymorphisrns, serving to create a variation in the repertoire of expressed T cell receptor genes. These repertoire differences may play a fundamental role in disease susceptibility.A study was therefore undertaken to establish whether linkage exists between the HIA system or the T cell receptor genes and a putative susceptibility gene for tuberculosis.Polymorphisrns of these genetic markers were examined in three Cape Coloured multiplex families, affected individuals having culture-positive pulmonary tuberculosis.HLA haplotypes were derived from serological typing of peripheral leucocytes from each individual. B-Iymphoblastoid cell lines were established from each family member. DNA was then extracted and digested with a variety of restriction endonucleases. After gel electrophoresis and Southern blotting, the DNA fragments were probed with a panel of T cell receptor cDNA probes, revealing the allelic polymorphisms.Linkage analysis was done using the Liped computer programme and Lad scores were determined for each marker locus using various genetic models. Haplotypes were also established for the T cell receptor genes and used in the linkage analysis.Although most of the Lad scores fell within the indeterminate range, a cumulative Lod score of 1.79 was obtained from the allele generated by the EcoRV/a2 enzyme/probe combination under a recessive model with 50% penetrance. This represents odds of about 52: 1 in favour of linkage between the T cell receptor a gene and a putative susceptibility gene to tuberculosis.

The purpose of this study was to ascertain if there are similarities and differences that differentiate one paramedical group from another in the realm of non-intellectual characteristics. Senior students and successful practitioners in four paramedical groups selected were Registered Nurse. Licensed Vocational Nurse, medical Assistant, and Dental Assistant.

This study was designed to determine if a need exists to teach decision making skills to paramedic students in San Bernardino County.