Typescript. / Thesis (Ph. D.)--University of Hawaii, 1970. / Bibliography: leaves [190]-196. / x, 196 l illus

Halle, Universiẗat, Diss., 2001.

No description available.

Saprolegnia parasitica is a pathogenic oomycete that cause saprolegniosis. Freshwater fish like salmon and trout species are particularly vulnerable to infection, which is characterized by cotton-like grayish mycelial growth on the surface of the fish. Currently, an effective treatment for this disease is not available. This pathogen has a great impact on freshwater fish species world-wide. An initial step to keep this devastating disease at bay is the ability to detect the responsible pathogen, so that appropriate actions could be taken before it becomes widespread. The development of molecular tools that will accurately and rapidly detect S. parasitica is the main goal of this project. This project is divided into two main sections. The first section describes initial marker design efforts that were focused on the internal transcribed spacer (ITS) regions. Efforts were also made for the collection of field samples and the generation of our own ITS data that includes a number of Saprolegnia spp. Compiled sequence data allowed the identification of unknown samples and the adoption of the clade taxonomic system that other researchers had established for species designations. The accumulated sequence data helped to clarify the taxonomy within the genus Saprolegnia and complemented previous studies. The design of broad specificity PCR primers also allowed a quick initial detection of Saprolegnia spp., which could then be identified to species either by determining ITS nucleotide sequence or by a subsequent step of RFLP marker. Isolates sequence data in the compiled sample collection could be used for validation purposes in further marker development. The second section of the project described the development of higher specificity molecular markers for the detection of S. parasitica. These were based on the study of three different gene loci as potential markers. These included the Pumilio RNA-binding protein (Puf), Glutathionylspermidine synthetase (Gsp) and the thiazole biosynthetic enzyme (Thi4). The nucleotide sequence of each locus was studied to develop suitable PCR primers that were then refined through testing against our isolate collection to improve their specificity for the target species. Saprolegnia parasitica-specific markers were developed for the Puf and Gsp loci and these were further evaluated using our field collected samples. / Graduate

Typescript. / Thesis (Ph. D.)--University of Hawaii at Manoa, 1990. / Includes bibliographical references (leaves 110-115) / Microfiche. / xiv, 115 leaves, bound ill. (some col.) 29 cm

Thesis (M.S.)--West Virginia University, 2008. / Title from document title page. Document formatted into pages; containsvii, 73 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 66-68).

Thesis (M.S.)--West Virginia University, 2004. / Title from document title page. Document formatted into pages; contains viii, 72 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 65-67).

Orientador: Edson Luiz Furtado / Banca: Nilton Luiz de Souza / Banca: Marcos Antonio Machado / Resumo: Phytophthora parasitica, principal causador da gomose dos citros, é um importante patógeno dos citros provocando danos em viveiros e no campo. Programas de melhoramento visando obtenção de plantas resistentes a P. parasitica exigem informações detalhadas sobre o tipo de herança e a localização de genes de resistência no genoma. Fontes de resistência às doenças podem ser encontradas em gêneros correlatos a citros como Poncirus sp. O presente estudo teve como objetivos detectar e verificar a estabilidade de marcadores moleculares e locos controladores de características quantitativas (QTLs) ligados à resistência a gomose em uma progênie F1 obtida do cruzamento entre Citrus sunki vs. Poncirus trifoliata 'Rubidoux'. As avaliações fenotípicas de três conjuntos de dados de 3 épocas de avaliação distintas foram incorporadas às informações dos mapas de ligação estabelecidos através de marcadores moleculares do tipo RAPD. Plantas jovens foram inoculadas com o patógeno, usando o método do disco e avaliadas medindo-se o comprimento da lesão. A estratégia do 'pseudo-testcross' foi adotada como delineamento genético. Os QTLs foram mapeados utilizando o método do mapeamento por intervalo composto (CIM) com o programa QTLCartographer v.1.25. A partir das média de todos os experimentos, foram identificadas 5 posições nos grupos de ligação II, III e V de P. trifoliata associadas à gomose de Phytophthora. Em 2 posições observou-se uma correlação na detecção de QTLs para as avaliações realizadas em duas das três épocas. Demonstrado desta forma a ocorrência da interação genótipo ambiente. / Abstract: P. parasitica, is the most important main causal agent of Citrus Phytophthora gummosis in Brazil and hve caused damage in nurseries and orchards. Improving resistence programs to get resistant to P. parasitica have been detailed information about the inheritance and gene localization resistance. Source of disease resistance can be found in correlated genera like Poncirus trifoliata. The present study had the objective of detecting molecular markers associated to quantitative trait loci (QTL) for resistance to Citrus Phytophthora gummosis using F1 lineage obtained between Citrus sunki vs. Poncirus trifoliata 'Rubidoux' cross. Phenotypical evaluations will be incorporated in linkage maps established through RAPD molecular markers. Young plants were inoculated with P. parasitica, using the disc method and evaluated after one month measuring lesions length. Pseudo-testcross strategy was be used for genetic outlining. All this information were evaluated in specific genetic-statistical programs. QTLs were mapped using the method of the maps for composed interval (CIM) with the program QTLCartographer v.1.25. CIM. Starting from the average of all of the experiments, they were identified 2 positions in the group of connection II of P. trifoliata associated with gomose of Phytophthora. In each area a correlation was observed in the detection of QTLs for the evaluations accomplished in two of the three times... (Complete abstract, click electronic address below). / Mestre

Made available in DSpace on 2014-06-11T19:28:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-06-25Bitstream added on 2014-06-13T20:58:39Z : No. of bitstreams: 1 boava_lp_me_botfca.pdf: 497651 bytes, checksum: 37f114f566262afe532ca550bf9ecb9f (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Universidade Estadual Paulista (UNESP) / Phytophthora parasitica, principal causador da gomose dos citros, é um importante patógeno dos citros provocando danos em viveiros e no campo. Programas de melhoramento visando obtenção de plantas resistentes a P. parasitica exigem informações detalhadas sobre o tipo de herança e a localização de genes de resistência no genoma. Fontes de resistência às doenças podem ser encontradas em gêneros correlatos a citros como Poncirus sp. O presente estudo teve como objetivos detectar e verificar a estabilidade de marcadores moleculares e locos controladores de características quantitativas (QTLs) ligados à resistência a gomose em uma progênie F1 obtida do cruzamento entre Citrus sunki vs. Poncirus trifoliata 'Rubidoux'. As avaliações fenotípicas de três conjuntos de dados de 3 épocas de avaliação distintas foram incorporadas às informações dos mapas de ligação estabelecidos através de marcadores moleculares do tipo RAPD. Plantas jovens foram inoculadas com o patógeno, usando o método do disco e avaliadas medindo-se o comprimento da lesão. A estratégia do 'pseudo-testcross' foi adotada como delineamento genético. Os QTLs foram mapeados utilizando o método do mapeamento por intervalo composto (CIM) com o programa QTLCartographer v.1.25. A partir das média de todos os experimentos, foram identificadas 5 posições nos grupos de ligação II, III e V de P. trifoliata associadas à gomose de Phytophthora. Em 2 posições observou-se uma correlação na detecção de QTLs para as avaliações realizadas em duas das três épocas. Demonstrado desta forma a ocorrência da interação genótipo ambiente. / P. parasitica, is the most important main causal agent of Citrus Phytophthora gummosis in Brazil and hve caused damage in nurseries and orchards. Improving resistence programs to get resistant to P. parasitica have been detailed information about the inheritance and gene localization resistance. Source of disease resistance can be found in correlated genera like Poncirus trifoliata. The present study had the objective of detecting molecular markers associated to quantitative trait loci (QTL) for resistance to Citrus Phytophthora gummosis using F1 lineage obtained between Citrus sunki vs. Poncirus trifoliata 'Rubidoux' cross. Phenotypical evaluations will be incorporated in linkage maps established through RAPD molecular markers. Young plants were inoculated with P. parasitica, using the disc method and evaluated after one month measuring lesions length. Pseudo-testcross strategy was be used for genetic outlining. All this information were evaluated in specific genetic-statistical programs. QTLs were mapped using the method of the maps for composed interval (CIM) with the program QTLCartographer v.1.25. CIM. Starting from the average of all of the experiments, they were identified 2 positions in the group of connection II of P. trifoliata associated with gomose of Phytophthora. In each area a correlation was observed in the detection of QTLs for the evaluations accomplished in two of the three times... (Complete abstract, click electronic address below).

University, Diss., 2003--Jena.