A 2-year grazing study was conducted to evaluate the growth performance, ruminal fermentation, carcass characteristics, and fatty acid compositions in subcutaneous adipose tissue of beef steers grazing tall fescue (Festuca arundinacea Shreb.; TF) pastures without or with N fertilization. Nitrogen fertilization increased crude protein concentration of TF pasture and average daily gain of beef steers. Increase in total volatile fatty acids (VFA) and ammonia-N (NH3-N) concentrations were detected in steers grazing fertilized TF. In comparison with steers on feedlot, pasture-finished steers had greater proportions of cis-9, trans-11 CLA and C18:3 n-3, but lower n-6:n-3 ratio in adipose tissue. In the first in vitro study using 2 energy supplements [corn or dried distillers grains with solubles (DDGS)] and 4 pasture mixture forages [TF without or with N fertilizer (TF˗NF or TF+NF), TF-alfalfa mixture (TF+AF), and TF-birdsfoot trefoil mixture (TF+BT)], we found that corn supplementation increased total VFA and propionate concentrations, while DDGS supplementation decreased total VFA concentrations. Lower NH3-N concentration and methane (CH4) production were observed due to energy supplementation, in particular when corn grain was supplemented. Similar NH3-N:VFA ratios were detected in the cultures fed the TF+NF and the TF+BT. This result indicates that the TF+BT had similar fermentation efficiency on in vitro ruminal metabolism compared with the TF+NF. The second in vitro study was performed to investigate the effects of grass-to-legume ratios of 3 different TF-legume mixed diets on in vitro fermentation characteristics in continuous cultures. Propionate concentration increased with the increasing of legume proportion in the mixed diets. The greatest propionate concentration was shown by cultures fed the TF+CM, while the TF+AF and the TF+BT maintained a similar propionate concentration. Increasing legume proportion in the forage diets also increased NH3-N concentration, but decreased CH4 production in the cultures. Further decrease of CH4 production was recorded when the TF+BT was fed to the cultures. Overall results from the grazing study demonstrate that N fertilizer can improve nutrient quality of TF as well as growth performance of grazing steers, while the in vitro studies showed positive effects of grass-legume mixture diets on in vitro microbial metabolism by improving ruminal fermentation and reducing CH4 production.

Dairy producers across the country are evaluating the effectiveness of management intensive grazing (MIG) systems as a means of reducing the economic pressures of confinement feeding and manure handling. Systems using MIG have been promoted as an environmentally safer way of managing nutrient balance on Center. However, little research has been conducted to evaluate how these systems affect phosphorus (P) loss from the Center through runoff and forage removal. The goal of this study was to investigate the effects of forage type and manure density on P levels in runoff from release plots that simulated a MIG dairy system. Two forage treatments were planted on runoff release plots and applied with three manure density treatments and four replications of each combination for a total of 24 plots. Orchardgrass (Dactylis glomerata) and a broad-leafed forage treatment consisting of buckhorn plantain (Plantago lanceolata), red clover (Trifolium pratense), and alfalfa (Medicago sativa) were planted on the runoff release plots in early spring. Manure densities with no manure (control), a density simulating a low stocking density (low), and a density simulating high stocking density (high) were applied to plots during grazing simulations. Six rainfall simulations were conducted over the season simulating grazing conditions when forage was removed and re-growth conditions when forage had reached its target re-growth height before re-grazing occurred. Thirty minutes of runoff was collected from the runoff release plots to determine first flush phosphorus losses from the various treatments. Mass losses of total phosphorus (TP) for manure treatments in series 1 were significant due to plot installation disturbance. No significant effect for ortho-phosphorus (ortho-P), particulate phosphorus (PP) or TP concentration or mass losses were observed for other simulation series. Significantly higher mass losses were observed in series 1 for both forage treatments, again due to installation disturbance. Seasonal mass losses for TP were significantly lower for the orchardgrass treatment. Forage mass removal over the season increased as the stands established. Orchardgrass treatments appeared to control P losses earlier in the season, while broadleaf treatments observed a steady increase in ortho-P losses up to series 5. Both mass and concentration losses of TP were controlled earlier in the season by orchardgrass, while PP showed no significant mass or concentration loss effects. / Master of Science