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Vasculariza??o uterina atrav?s da ultrassonografia color doppler em ?guas com endometrite bacteriana induzida submetidas a tratamento / Characterization of uterine vascularization using color Doppler in equine endometritisS?, Marcus Andr? Ferreira 23 August 2017 (has links)
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Previous issue date: 2017-08-23 / Objectives of this study were to characterize the endometrial vascular perfusion of mares with bacterial endometritis using color Doppler ultrasonography and submitted to phytotherapeutic treatment. 19 crossbred and Mangalarga Marchador mares without endometritis were used. ImageJ 1.46r? was applied to evaluate the images. Additionally, subjective vascular uterine perfusion was estimated analyzing the percentage of color Doppler signals present in the mesometrial, myometrium and endometrium, in longitudinal section of the uterine body and transverse section of the uterine horns. Step 1. The mares (n=20) were submitted to intrauterine inoculation with Escherichia coli cepa from the equine uterus. The color Doppler ultrasound sessions were performed in M0 (moment immediately prior to intrauterine inoculation) and M1 (24 hours after intrauterine inoculation). Step 2. The animals were divided into two groups: control group (n=10) and treated group (n=10) using phytotherapic solution Fitoclean? (Organnact Animal Health, Curitiba, Paran?, Brazil). Both groups, color Doppler ultrasonography was performed at time T1 (immediately before starting treatment), T2 (24h after treatment) and T3 (48h after treatment). Step 3. Mares in which pathogens were identified in the samples collected during stage 2 were submitted to antibiotic therapy by intrauterine infusion using Gentamicin. Seven days after the antibiotic therapy, a new color Doppler ultrasound examination was performed. For statistical analysis, the Tukey test, t Student and Anova test was applied to compare the means obtained in the different periods and Chi Square were apllied to evaluation of phytoterapic effect. Step 1. It was found that the mean values of vascularization were no moment (P<0.05). Bacterial growth was observed in all samples collected. Step 2. The mean value of vascularization at time T1 in both groups was significantly higher (P<0.05) than the obtained at moments 2 and 3. At the time T1, we observed a significant increase in portion 1, while T2 showed a significant decreasing in this portion. Step 3. According to the results obtained in the culture and antibiogram examinations performed during the step 2, 13 mares (65%, 13/20) were submitted to antibiotic therapy through intrauterine infusion with Gentamicin. After that treatment, the vascularization observed decreased related to M1. With these results, we can conclude that in these conditions is possible the use of color Doppler ultrasonography as a method for bacterial endometritis; It was not possible to correlate results obtained by color Doppler ultrasonography with the traditional findings for the diagnosis of endometritis. / Os objetivos do presente estudo foram caracterizar atrav?s da ultrassonografia color Doppler a perfus?o vascular endometrial de ?guas com endometrite bacteriana e submetidas a tratamento fitoter?pico. Para tanto, foram utilizadas 20 ?guas mesti?a e Mangalarga Marchador livres de endometrite. O programa ImageJ 1.46r? foi utilizado para avalia??o das imagens. Adicionalmente, a perfus?o vascular subjetiva do ?tero foi estimada levando-se em considera??o o percentual de sinais Doppler coloridos presentes no mesom?trio, miom?trio e endom?trio, em corte longitudinal do corpo uterino e transversal dos cornos uterinos. Etapa 1. Todas as ?guas (n=20) foram submetidas a inocula??o intrauterina com cepa de Escherichia coli proveniente de ?tero equino. As sess?es de ultrassonografia color Doppler foram realizadas em M0 (momento imediatamente anterior ? inocula??o intrauterina) e M1 (24 horas ap?s a inocula??o intrauterina). Etapa 2. Os animais foram divididos aleatoriamente em dois grupos: grupo experimental 1 (n=10) e grupo experimental 2 (n=10) com solu??o fitoter?pica Fitoclean? (Organnact Sa?de Animal, Curitiba, Paran?, Brasil). Em ambos os grupos, os exames de cultura uterina, antibiograma, citologia endometrial e ultrassonografia modo B e color Doppler foram realizados nos momentos T1 (imediatamente antes de iniciar o tratamento), T2 (24h ap?s o tratamento) e T3 (48h ap?s o tratamento). Etapa 3. As ?guas em que foram identificados agentes patog?nicos nas amostras coletadas durante a etapa 2, foram submetidas ? antibioticoterapia por infus?o intrauterina utilizando Gentamicina. Sete dias ap?s a realiza??o da antibioticoterapia, foi realizado novos exame de ultrassonografia color Doppler. Para an?lise estat?stica, foi aplicado o teste de Tukey, teste t de Student e Anova para compara??o das m?dias obtidas nos diferentes per?odos e Qui Quadrado para avalia??o do efeito do fitoter?pico. Etapa 1. Verificou-se que os valores m?dios de vasculariza??o no momento M1 foram significativamente superiores aos obtidos em M0 para as tr?s partes do ?tero (P<0,05). Houve crescimento bacteriano em todas as amostras coletadas. Etapa 2. O valor m?dio de vasculariza??o no momento T1 em ambos os grupos foi significativamente superior (P<0,05) aos obtidos nos momentos 2 e 3. No momento T1, observou-se aumento significativo no segmento 1, enquanto que em T2 observou-se uma redu??o significativa neste segmento. Etapa 3. De acordo com os resultados obtidos nos exames de cultura e antibiograma realizados ap?s o t?rmino da etapa 2, 13 ?guas (65%, 13/20) foram submetidas a antibioticoterapia atrav?s de infus?o intrauterina com Gentamicina. Ap?s este tratamento, a vasculariza??o observada apresentou grande redu??o em compara??o ao momento M1. Diante destes resultados, concluiu-se que nas condi??es deste estudo foi poss?vel utilizar a ultrassonografia modo Color Doppler como m?todo diagn?stico para a endometrite bacteriana; n?o foi poss?vel correlacionar resultados obtidos atrav?s da ultrassonografia color Doppler com os achado nos exames tradicionais para diagn?stico da endometrite.
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