Made available in DSpace on 2016-04-28T20:18:33Z (GMT). No. of bitstreams: 1 2009 - Claudia Borges da Costa.pdf: 2611847 bytes, checksum: bac6f485f20793fe627e66f8a53c973e (MD5) Previous issue date: 2009-06-26 / This experiment aimed at recognizing and quantifying the possible macro and microscopic tissue alterations that may occur with the application of bovine pericardium preserved in different concentrations of glutaraldehyde on the abdominal wall. Besides, it also intended to identify the possible interferences in the implant incorporation, according to the variation in the concentration of each solution. Fresh bovine pericardium, after cleaning and the removal of fat, were fractioned and treated with 98% glycerin solution for 30 days (control groups) or with 0.625%, 1% and 1.5% glutaraldehyde solution for 18 days (experimental groups). The fragments were implanted on the abdominal wall of 60 mice (n=15 per treatment) for 7, 14 and 30 days. After each of these periods, five animals of each group were sacrificed for collection, and macro and microscopic analysis of tissue samples. It was observed that the inflammatory reaction in the groups treated with glycerin and 0.625% glutaraldehyde was similar in terms of inflammatory cells infiltration and the repairing process. The groups whose implants were treated with 1% glutaraldehyde chronically presented (30 days) not only more intense fibrosis and necrosis, when compared to other glutaraldehyde groups, but also tissue destruction. These were also observed in the animals that received the pericardium treated with 1.5% glutaraldehyde, analyzed after 14 and 30 days, what leads to the conclusion that this concentration is unviable for the treatment of biological membranes. / Este experimento teve como objetivo reconhecer e quantificar as poss?veis altera??es teciduais macro e microsc?picas que podem ocorrer ap?s a aplica??o de peric?rdio bovino conservado em diferentes concentra??es de glutaralde?do em parede abdominal. Tamb?m foram identificadas poss?veis interfer?ncias na incorpora??o do enxerto de acordo com a varia??o da concentra??o da solu??o de tratamento. Peric?rdios bovinos frescos, ap?s limpeza e retirada de gordura, foram fracionados em fragmentos de 1cm2 e tratados com solu??o de glicerina 98% por 30 dias (grupos controle) ou com solu??o de gutaralde?do 0,625%, 1% e 1,5% por 18 dias (grupos experimentais). Os fragmentos foram implantados na parede abdominal de 60 camundongos (n=15 por tratamento) por 7, 14 e 30 dias e ap?s estes per?odos, cinco animais de cada grupo foram sacrificados para coleta e an?lise macro e microsc?pica das amostras teciduais. Observou-se que a resposta inflamat?ria dos grupos com tratamento com glicerina e com glutaralde?do 0,625% foi semelhante tanto na infiltra??o das c?lulas inflamat?rias quanto no processo de repara??o. Os grupos com implantes tratados com glutaralde?do 1% apresentaram cronicamente (30 dias) a presen?a de fibrose e necrose mais intensa na musculatura da parede abdominal, quando comparado com os outros grupos de glutaralde?do, al?m de destrui??o tecidual. Esta tamb?m foi observada nos animais que receberam peric?rdio tratado com glutaralde?do 1,5% e foram analisados com 14 e 30 dias, o que permitiu a conclus?o de que esta concentra??o ? invi?vel para o tratamento de membranas biol?gicas.