The rice leafroller Cnaphalocrocis exigua (Butler) in the only species of the rice leaf folder/roller complex in Fiji. It is one of the major rice pests in the country, its pest status being exacerbrated by the cultivation of high-yielding varieties with minimal pest resistance. Detailed studies on the biology of the moth, in particular on the development, emergence, mating and flight behaviour, effect of adult nutrition on fecundity, egg hatchability and longevity, effect of larval nutrition on larval survival, pupation, pupal weight, pupal eclosion, and fecundity, egg hatchability and longevity of resultant adults, parasitism, and population ecology of C. exigua were carried out in Fiji from 1985 to 1987, and from June to August 1990. This study showed that there are several parasitoids of C. exigua in Fiji, and of those the egg and larval parasitoids Trichogramma sp. and Trathala flavo-orbitalis (Cameron) respectively are significant biotic mortality factors. Their impact as natural suppressors of C. exigua in fields of high yielding rice varieties was, however, inadequate to prevent C. exigua reaching damaging levels. Since C. exigua responds strongly to rice varietal differences, plant nitrogen status, and seasonal factors, is suspected to utilise pheromones during mating, and has numerous biocontrol agents in South East Asia and the Philippines, it appears to be an excellent candidate for an integrated pest management (IPM) program in Fiji. Before IPM strategies can be formulated, however, further investigations on rice varietal resistance, exploitation of exotic parasitoids and pathogens, and detailed studies on the possible presence of sex pheromones need to be conducted. / Doctor of Philosophy (PhD)

[No Abstract]

Maternal behavior in rodents is dependent, at least in part, on prolactin acting in the brain. Pheromones carried by male mouse major urinary proteins lower serum prolactin levels in female mice. Therefore, we hypothesized that virgin female C57BL/6J mice housed in split cages, where they had pheromonal but not physical contact with a male, would show suppressed maternal behavior. Contrary to our hypothesis, we found split-cage housed females were significantly faster to retrieve 3 foster pups on the first and second day of maternal behavior testing compared to mice housed in individual cages. The advancement in maternal behavior was replicated when virgin females were simply exposed to male mouse urine-soaked bedding. Ovariectomising the mice, to remove the influence of steroid hormones, prior to placement in the split cages, prevented the pheromonal advancement of maternal behavior. The data infer that an ovarian steroid-dependent action of male mouse pheromones primes virgin female mice to express maternal behavior more rapidly when mouse pups are introduced. This effect required greater than 14 days exposure to male pheromones. Male mouse pheromones are reported to suppress prolactin secretion. However, serum prolactin levels in split-caged housed females, where they had pheromonal but not physical contact with a male, were only briefly lowered and became significantly elevated from 24 hours until 72 hours of pheromonal contact. Despite the early increases in prolactin after pheromone exposure, levels were significantly lower in the pheromone-exposed females when maternal behavior was tested after 21 days. It has been previously reported that prolactin is important in the onset of maternal behavior, but is not required for the ongoing maintenance of maternal behavior. We hypothesised that the hyperprolactinemia observed in the first 24-72 hours of pheromonai exposure had subsequently led to the enhanced maternal behavior. To test this we injected a group of individually-housed mice with slow release prolactin for 48 hours to simulate the period of hyperprolactinemia, and blocked prolactin secretion in a group of split-caged housed females with bromocriptine, and tested their maternal behavior 18 days later. The mice injected with prolactin had enhanced maternal behavior, compared to controls injected with a placebo. By contrast, bromocriptine inhibition of prolactin secretion completely prevented the pheromonal enhancement of maternal behavior. This suggests that the pheromonal advancement of maternal behavior is specifically mediated by a 48-hour period of sustained hyperprolactinemia. It has been previously shown that pregnancy increases neurogenesis in the subventricular zone in a prolactin-dependent manner. Therefore, as the male pheromone-induced advancement of maternal behavior is prolactin-dependent and takes some time to occur, we hypothesized that long-term pheromonal contact initiates mitogenesis in the subventricular zone. Split-caged housed mice showed a significant increase in BrdU-labeled cells in the subventricular zone after 7 days of contact which reduced to baseline levels by 14 days of contact. The mice injected with BrdU on day 7 of contact and killed 21 days later showed a significant increase in labeled cells in the accessory olfactory bulb compared to controls. The data suggest that male mouse pheromones initiate mitogenesis in the subventricular zone of virgin C57B6 mice, in an exposure-dependent manner, and that these cells travel via the rostral migratory stream to the accessory olfactory bulb. As with the effect on maternal behavior, the pheromone-induced increase in neurogenesis was steroid- and prolactin-dependent. During pregnancy and lactation in rodents, prolactin receptor expression is increased in the MPOA, an adaptive change, which could lead to an increased neuronal response to serum prolactin levels, which are high just prior to parturition, and consequently could underlie the enhanced maternal responses seen in late pregnancy and after parturition. It is known that systemic prolactin can access the brain, but it is also possible that there could be local synthesis of brain prolactin acting in an autocrine or paracrine manner. Therefore we hypothesized that the pheromonal-induced changes in maternal behavior are being mediated by altered prolactin receptor expression/sensitivity and/or increased production of brain prolactin. Using RT-PCR to measure levels of prolactin receptor and prolactin mRNA, we found changed expression of the 3 short forms and the long form of prolactin receptor mRNA in the arcuate nucleus, paraventricular nucleus, bed nucleus of the stria terminalis, and MPOA with either exposure to male pheromones or pups. We also found changes in prolactin mRNA in the MPOA and paraventricular nucleus after exposure to pups or male pheromones. The data suggest that altered levels of expression of the receptor, coupled with local production of brain prolactin acting in an autocrine or paracrine manner, may cause a net change in prolactin cell signaling, which leads to adaptive responses which ensure reproductive success. There is extensive evidence that dopamine is a key neurotransmitter mediating maternal behavior. In addition, there is some evidence that serotonin may also be involved in regulating maternal behavior. Therefore, we hypothesised that the pheromonal-induced changes in maternal behavior would be associated with increased dopaminergic and/or serotonergic neuronal activity in the MPOA and other areas of the brain implicated in maternal behavior expression. Using HPLC to measure levels of dopamine and serotonin and their respective metabolites, we found a significant increase in serotonergic and dopaminergic neuronal activity in the MPOA of virgin female C57BL/6J mice after 24 hours of pheromonal contact. The neuronal activity returned to basal levels after exposure to pups. The data suggest that male mouse pheromones increase serotonergic and dopaminergic neuronal activity in the MPOA, but that dopamine and serotonin levels are tightly regulated within strict parameters dependent on what physical stimuli the female is receiving. Changes in prolactin levels are associated with altered responses to anxiety. There is an increased risk of anxiety and depression with sustained periods of hyperprolactinemia, and in the postpartum period, where there are fluctuations in prolactin levels, there is an increased risk of mood disorders. As pheromones change both serum and brain prolactin levels and prolactin modulates anxiety, we hypothesised that female mice exposed to pheromones would show altered behavioral responses to a standardized test of anxiety. We found that male pheromone-exposed mice showed decreased levels of anxiety on an elevated plus maze compared to individually housed controls. Female mice exposed to female pheromones displayed 2 disparate responses to the plus maze. One female from each cage showed increased anxiety, while her cage-mate showed decreased anxiety, yet both groups of female mice showed impaired maternal behavior. We infer, that in this model, male pheromones decrease anxiety, but anxiety and expression of maternal behavior are not directly correlated. The major signal transduction pathway activated by prolactin binding to its receptors in the brain is the JAK/STAT signalling pathway, and in some neurons, in particular, the STAT5B pathway. The expression of prolactin and its receptor affect maternal behavior in mice. Therefore, we hypothesised that if the JAK/STAT STAT5B pathway is involved in maternal behavior, then STAT5B-deficient mice would have altered maternal behavior. We found that there were no significant differences in expression of full maternal behavior between the STAT5B-deficient mice and wild-type controls. The data suggest that STAT5B is not required for normal expression of maternal behavior. We propose that the prolactin-mediated pheromonal increase in neurogenesis, alteration in monoamine synthesis, and alteration of prolactin and prolactin receptor mRNA levels facilitate expression of enhanced maternal behavior. We further propose that the pheromonal decrease in anxiety does not mediate enhanced maternal behavior. In addition, we propose that prolactin does not mediate maternal behavior through STAT5B. While pheromones have previously been reported to exert powerful actions on the reproductive system, these results demonstrate for the first time that male pheromones potentially complement the prolactin-mediated establishment of maternal behavior.

<p>Pheromones are chemicals from conspecifics that affect innate behavior or hormonal changes. In mammals, the vomeronasal organ (VNO) is thought to play a prominent role in detecting pheromones; the vomeronasal sensory neurons (VSNs) express three families of seven-transmembrane G-protein coupled receptors (GPCRs): the V1Rs, V2Rs, and FPRs, in two molecularly and spatially-distinct regions. In mice, VSNs that express the V2Rs are thought to detect peptide cues, including MHC-presenting peptides, major urinary proteins (MUPs), and exocrine gland-secreting peptides (ESPs). They are thought to be involved in various pheromone-mediated behaviors and physiological changes, such as mating, aggression, and selective pregnancy block. In order to understand how pheromones are detected by the vomeronasal receptors, it is essential to know which receptors are activated by a given chemical. However, identifying cognate ligands for the V2Rs has been challenging, partly because they are poorly localized to the surface of heterologous cells. Here, we show that the calreticulin chaperone family members play a crucial role in trafficking V2Rs. A calreticulin homologue, calreticulin4 is specifically expressed in the VNO, while calreticulin expression level is low. Depleting calreticulin expression in HEK293T cells allows V2Rs to be trafficked to the cell surface, whereas expression of calreticulin4 does not block the trafficking of the V2Rs. Using this knowledge, we have established a heterologous cell system to functionally identify the V2Rs and demonstrate that the ESP family members can differentially activate the V2Rs. We also show the large extracellular domain of the V2Rs plays a crucial role in ligand selectivity. Our results provide a platform to characterize ligand selectivity of the V2Rs and suggest that a unique mechanism involving calreticulins regulates the functional expression of the V2Rs.</p> / Dissertation

No description available.

Electronic noses are targeted at determining odour character in a fashion that emulates conscious odour perception in mammals. The intention of this study was to develop an organisational framework for electronic noses and deploy a sample cheese odour discriminator within this framework. Biological olfactory systems are reviewed with the purpose of extracting the organisational principles that result in successful olfaction. Principles of gas handling, chemoreception, and neural processing are considered in the formulation of an organisational framework. An electronic nose is then developed in accordance with the biologically inspired framework. Gas sensing is implemented by an array of six commercially available (tin oxide) semiconductor sensors. These popular gas sensors are known to lack stability thus necessitating hardware and signal processing measures to limit or compensate for instability. An odorant auto-sampler was developed to deliver measured amounts of odorant to the sensors in a synthetic air medium. Each measurement event encodes a simulated sniff, and is captured across six sensor channels over a period of 256 seconds at a sampling rate of 1Hz. The simulated sniff captures sensor base references and responses to odorant introduction and removal. A technique is presented for representation and processing of sensor-array data as a two-dimensional (2D) image where one dimension encodes time, and the other encodes multi-channel sensory outputs. The near optimal, computationally efficient 2D Discrete Cosine Transform (DCT) is used to represent the 2D signal in a decorrelated frequency domain. Several coefficient selection strategies are proposed and tested. A heuristic technique is developed for the selection of transform domain coefficients as inputs to a non-linear neural network based classifier. The benefits of using the selection heuristic as compared to standard variance-based selection are evident in the results. Benefits include: significant dimensionality reduction with concomitant reduction in classifier size and training time, improved generalisation by the neural network and improved classification performance. The electronic nose produced a 99.1% classification rate across a set of seven different cheeses. / Thesis (M.Sc.Eng.)-University of Natal, Durban, 2003.

The responses of the saw-toothed grain beetle, 0ryzaephilus surinamensis to food odours and pheromones were investigated at both sensory and behavioural levels to give an integrated picture of olfaction, and the importance of odours in this species. The sensory capability of the antenna was assessed by a study of the structure, distribution and function of sensilla using SEM and TEM. Seven different sensilla types, including 3 with an olfactory function, were identified, although the total number of olfactory sensilla was low compared with those for mechanoreception. Antennal responses to odours were studied using both the electroantennogram (EAG) technique and single-cell recordings, whilst a 2-choice pitfall assay and a single-insect arena test were used to investigate behavioural responses. Food-produced volatiles from carob pods were found to produce large amplitude antennal responses and attraction in behavioural assays. Analysis of such volatiles by coupled gas chromatography-electroantennography (GC-EAG) demonstrated at least 3 active components, of which one was identified as hexanoic acid. Attraction to food odour was found to vary with insect age, and this correlated with similar variation in the EAG response, suggesting a role for peripheral receptor sensitivity in modulating insect behaviour. Beetle-produced volatiles also caused antennal responses and attraction of conspecific individuals of both sexes, thus acting as an aggregation pheromone. The active components were identified by GC-EAG as (Z,Z)-3,6-dodecadien-11-olide, (Z,Z)-3,6-dodecadienolide and (Z,Z)-5,8-tetradecadien-13-olide. These act as a multicomponent pheromone, with separate antennal receptors for each component. Although no sexual differences in antennal responses were found, consistently more females than males were attracted to the pheromone. This suggested a partial sex pheromone function, as the pheromone was shown to be male-produced. However, the ratio of females:males attracted was found to vary with the blend ratio tested. Thus if males are able to alter the blend they produce, the pheromone may act at different times as either a sex pheromone or an aggregation pheromone. Finally, the functional and evolutionary importance of odours to this insect are discussed.

No description available.

Thesis (Ph. D.)--University of Nevada, Reno, 2007. / "August 2007." Includes bibliographical references. Online version available on the World Wide Web.

Licentiatavhandling (sammanfattning) Alnarp : Sveriges lantbruksuniversitet. / Härtill 2 uppsatser.