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Studies on Incorporation of 14C into Carrageenan and Methods of Localizing Carrageenan in Animal TissuesRicher, Suzanne M. 10 1900 (has links)
<p> Lambda carrageenan when injected subcutaneously causes the formation of a connective tissue granuloma. Initially there is a proliferation of connective tissue elements up to about fourteen days followed by regression so that by six weeks most collagenous tissue has
disappeared and been replaced by adipose tissue. Lambda carrageenan has been identified in the granuloma by staining reactions with toluidine blue and other stains for acid polysaccharides. The present study was undertaken to localize the carrageenan by means of fluorescent antibody and autoradiography. For this purpose labelling of carrageenan by photoassimilation of 14CO2 into carrageenan was done. Different parameters affecting the incorporation of 14C into the carrageenan fractions were studied.</p> / Thesis / Master of Science (MSc)
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Diel Mediated Populus balsamifera Transcriptome Components Test the Impacts of Artificial Nighttime LightingSkaf, Joseph 27 November 2012 (has links)
Artificial nighttime lighting (ANL) is known to adversely affect animals, but little is known what the consequences are to plants. Two genotypes of Populus balsamifera, a common urban tree, were used to investigate how ANL impacts plants. While the two genotypes varied in their physiological sensitivity to ANL, poorer levels of net leaf carbon assimilation compared to control samples suggested that ANL perturbed the perception of time of day for these plants. Gene set analysis on a subset of PopGenExpress microarray samples identified time of day specific processes in P. balsamifera, and a set of candidate ANL-sensitive genes were identified from these. Transcript measurements from the two genotypes revealed that ANL affects plants at the molecular level, for the diel cycling of the putative ANL-sensitive genes was perturbed. Together, these results suggest that ANL affects plants at the physiological and molecular level by perturbing their perception of time of day.
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Diel Mediated Populus balsamifera Transcriptome Components Test the Impacts of Artificial Nighttime LightingSkaf, Joseph 27 November 2012 (has links)
Artificial nighttime lighting (ANL) is known to adversely affect animals, but little is known what the consequences are to plants. Two genotypes of Populus balsamifera, a common urban tree, were used to investigate how ANL impacts plants. While the two genotypes varied in their physiological sensitivity to ANL, poorer levels of net leaf carbon assimilation compared to control samples suggested that ANL perturbed the perception of time of day for these plants. Gene set analysis on a subset of PopGenExpress microarray samples identified time of day specific processes in P. balsamifera, and a set of candidate ANL-sensitive genes were identified from these. Transcript measurements from the two genotypes revealed that ANL affects plants at the molecular level, for the diel cycling of the putative ANL-sensitive genes was perturbed. Together, these results suggest that ANL affects plants at the physiological and molecular level by perturbing their perception of time of day.
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