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31	Indukovaná RNAi proti esenciálním genům metabolismu dusíku jako nástroj pro kontrolu GM rostlin / Inducible RNAi against essential genes of nitrogen metabolism as a tool for control of GM plants Kobercová, Eliška January 2017 (has links) Uncontrolled spreading of genetically modified (GM) plants is one of the main concerns about their cultivation. Inducible RNA interference against an essential gene could be a tool for control of GM plants. After spraying with a chemical inducer, the essential gene will be silenced so the treated GM plant will die. For testing this strategy we chose two key enzymes of nitrogen metabolism, glutamate synthase (GOGAT) and glutamine synthetase (GS). GS processes ammonium ions into glutamine, then GOGAT transfers the amide group from glutamine to 2-oxoglutarate to form two glutamates. GS/GOGAT cycle is the main pathway for assimilation of ammonium ions, which could be toxic to plants in a higher concentration. Disruption of ammonium assimilation during photorespiration causes a strong inhibition of photosynthesis. The aim of this work was to describe the effects of silencing GOGAT and GS genes in Arabidopsis thaliana. To induce silencing, RNAi hairpin constructs under a control of constitutive or estradiol-inducible promoter were prepared. In selected independent transformants with the inducible hairpin against GOGAT, chlorosis and reduced growth were observed after the estradiol treatment in in vitro conditions. However, the spraying with estradiol was tricky, at the whole plant level, the induction of...
32	Änderung der Glycindecarboxylase- und Serinhydroxymethyltransferase-Aktivität in Blättern: / Wirkungen auf die Photosynthese und den Stickstoff- und Kohlenstoff-Metabolismus / Manipulation of glycine decarboxylase and serine hydroxymethyl transferase activity in leaves / Effects on photosynthesis and N- and C-metabolism Antonicelli, Gerardo Esteban 26 January 2005 (has links) No description available. 570 Biowissenschaften, Biologie Mathematics and Computer Science Photorespiration Glycindecarboxylase Serinhydroxymethyltransferase Photosynthese Stickstoffmetabolismus Kohlenstoffmetabolismus Solanum tuberosum L. Nicotiana tabacum L. photorespiration glycine decarboxylase serine hydroxymethyl transferase photosynthese nitrogen and carbon metabolism Solanum tuberosum L. Nicotiana tabacum L. 42.41 Pflanzenphysiologie 42.43 Pflanzengenetik 42.13 Molekularbiologie

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Indukovaná RNAi proti esenciálním genům metabolismu dusíku jako nástroj pro kontrolu GM rostlin / Inducible RNAi against essential genes of nitrogen metabolism as a tool for control of GM plants