In the years 2005 - 2006 it was practised typological maping of selected meadow and pasture areas in Kardašova Řečice (439 m n.m.). The selected areas of permanent grasslands were separated into parts by growth types, where it was observed: projective dominance and frequency of individual species, the amount of primary production of biomass, the underground water level.

The aim of the work was to describe the biodiversity of model groups of organisms (vascular plants, epigeic beetles and small mammals) in six transect crossing the two year old high way situated close to the town Písek (South Bohemia). The different methods were used for studied groups (phytocenological mapping, pitfall traps and mousetraps). The results documented the possibility of all studied organisms for the quick recolonization of new biotopes beside of the high way.