Thesis--University of Rochester, 1965.

Indiana University-Purdue University Indianapolis (IUPUI) / The concepts of using a pit and fissure sealant material to prevent dental caries have been well established in dental research. Effectiveness of a pit and fissure sealant material is limited to its ability to remain bonded to the occlusal surfaces. Adding a dentin-bonding agent between the etched enamel and the sealant material has been demonstrated as a way of optimizing bond strength in the face of moisture and salivary contamination. The purpose of this study was to examine if there was a difference in fissure penetration or microleakage between a conventional pit and fissure sealant or a flowable composite when used as a pit and fissure sealant, while using three different adhesive systems. Delton Direct Delivery System (Dentsply), an opaque, light cured pit and fissure sealant and the commercially available flowable composite, Revolution Formula 2 (Kerr) was selected for this study. Three clinically used adhesive systems selected for this study were: conventional phosphoric acid etching; Opti-bond Solo Plus(Kerr), a single bottle system; and Prompt L-Pop (3M ESPE), an all-in-one primer adhesive. One hundred fifty extracted caries-free third molars, selected for well-defined occlusal pits, were randomly divided into six treatment groups. Fissure penetration and microleakage was examined after immersion of the treated teeth in 5.0-percent methylene blue solution for 18 hours; the teeth were removed and thoroughly cleaned. Mesial and distal flat-ground sections were obtained and examined at X20; microleakage was recorded as either present or absent, and penetration was recorded as either complete or incomplete. The interaction between the material and the adhesive system was non-significant based on the logistic regression model for the penetration and the microleakage, so that only the main effects of material and adhesive system were included in the final model. Enamel conditioning with the total-etch and single-bottle adhesive system provided consistently microleakage resistance when compared with the use of the all-in-one bonding system. The all-in-one adhesive system demonstrated the most microleakage regardless of the material used for the pit and fissure sealant. The hypothesis of this thesis was that there would be no significant difference in fissure penetration or microleakage between the conventional pit and fissure sealant or the flowable composite, regardless of the adhesive system used. The results of this study support the hypothesis that there was no significant difference in fissure penetration between the materials. The result did demonstrate that there was significant difference in microleakage between the three different adhesive systems used.

Indiana University-Purdue University Indianapolis (IUPUI) / The concepts of using a pit and fissure sealant material to prevent dental caries have been well established in dental research. Effectiveness of a pit and fissure sealant material is limited to its ability to remain bonded to the occlusal surfaces . Adding a dentin-bonding agent between the etched enamel and the sealant material has been demonstrated as a way of optimizing bond strength in the face of moisture and salivary contamination. The purpose of this study was to examine if there was a difference in fissure penetration or microleakage between a conventional pit and fissure sealant or a flowable composite when used as a pit and fissure sealant, while using three different adhesive systems. Delton Direct Delivery System (Dentsply), an opaque, light cured pit and fissure sealant and the commercially available flowable composite, Revolution Formula 2 (Kerr) was selected for this study. Three clinically used adhesive systems selected for this study were: conventional phosphoric acid etching; Opti-bond Solo Plus(Kerr), a single bottle system; and Prompt L-Pop (3M ESPE), an all-in-one primer adhesive. One hundred fifty extracted caries-free third molars, selected for well-defined occlusal pits, were randomly divided into six treatment groups. Fissure penetration and microleakage was examined after immersion of the treated teeth in 5.0-percent methylene blue solution for 18 hours; the teeth were removed and thoroughly cleaned. Mesial and distal flat-ground sections were obtained and examined at X20; micro leakage was recorded as either present or absent, and penetration was recorded as either complete or incomplete. The interaction between the material and the adhesive system was non-significant based on the logistic regression model for the penetration and the microleakage, so that only the main effects of material and adhesive system were included in the final model. Enamel conditioning with the total-etch and single-bottle adhesive system provided consistently microleakage resistance when compared with the use of the all-in-one bonding system. The all-in-one adhesive system demonstrated the most microleakage regardless of the material used for the pit and fissure sealant. The hypothesis of this thesis was that there would be no significant difference in fissure penetration or microleakage between the conventional pit and fissure sealant or the flowable composite, regardless of the adhesive system used. The results of this study support the hypothesis that there was no significant difference in fissure penetration between the materials. The result did demonstrate that there was significant difference in microleakage between the three different adhesive systems used.

Thesis (M.S.D.)--Indiana University School of Dentistry, 2009. / Title from PDF t.p. (viewed Feb. 5, 2010) Advisor(s): Michael A. Cochran, Chair of the Research Committee, Armando E. Soto Rojas, Margherita R. Fontana, Timothy Carlson, Tien-Min Gabriel Chu. Curriculum vitae. Includes abstract. Includes bibliographical references (leaves 56-61).

Thesis (M.S.)--School of Dentistry. University of Missouri--Kansas City, 2008. / "A thesis in oral biology." Advisor: Mary P. Walker. Typescript. Vita. Title from "catalog record" of the print edition Description based on contents viewed Aug. 07, 2009. Includes bibliographical references (leaves 39-45). Online version of the print edition.

Made available in DSpace on 2014-06-11T19:33:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-09-06Bitstream added on 2014-06-13T20:24:31Z : No. of bitstreams: 1 vieira_aem_dr_araca.pdf: 862313 bytes, checksum: 73131cab7febe9ed9ca73177596b76b0 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Bisco Inc., Il, Usa / Bosworth Company, Il, Usa / O presente trabalho avaliou a capacidade da microtomografia de raios-X (XMT) laboratorial e síncrotron para mensurar coeficientes de absorção linear em escala micrométrica em estudos in vitro utilizando dentes bovinos e ciclagem de pH. Sendo assim, este estudo foi desenvolvido em cinco manuscritos, com os seguintes objetivos: traçar parâmetros e determinar um protocolo para a quantificação do conteúdo mineral de esmalte bovino utilizando XMT laboratorial e síncrotron; analisar a estrutura da junção amelo-dentinária em incisivos bovinos utilizando XMT síncrotron; determinar o potencial anti-cárie do fosfato de cálcio amorfo (ACP) presente em selantes de fóssulas e fissuras, utilizando dentes bovinos, ciclagem de pH, microdureza de superfície e XMT síncrotron; e avaliar, através de análise de microdureza de superfície e XMT síncrotron, a capacidade de uma pasta contendo CPP-ACP para ativar a remineralização do esmalte dental. Os resultados obtidos mostram que: a XMT propiciou a determinação da quantidade mineral com uma precisão mais adequada, sendo a melhor resolução espacial obtida através da luz síncrotron; a junção amelo-dentinária apresentou-se menos mineralizada que a dentina ou esmalte; o fosfato de cálcio amorfo presente nos materiais testados não foi capaz de inibir a desmineralização ou ativar a remineralização do esmalte bovino, em ciclagem de pH, tanto quanto os íons flúor. Conclui-se que a XMT é capaz de determinar pequenas alterações na mineralização do dente bovino após ciclagem de pH. / This study evaluated the ability of laboratory and synchrotron X-ray microtomography (XMT) for measuring linear attenuation coefficient at micrometer scale of in vitro studies using bovine teeth and pH-cycling. For that, this study included five papers, with the following aims: to define parameters and a protocol for quantification of mineral content of bovine enamel using laboratory and synchrotron XMT; to analyze the dentinoenamel junction (DEJ) structure in bovine incisors using synchrotron XMT; to determine the anti-caries potential of amorphous calcium phosphate (ACP) in pit and fissure sealants, using bovine teeth, pH-cycling, surface hardness and synchrotron XMT; and to evaluate, through surface hardness analysis and synchrotron XMT, the ability of a CPP-ACP paste to enhance enamel remineralization. The results showed that: XMT provided mineral quantification with an adequate precision; synchrotron microtomography provided the best spatial resolution; DEJ was less mineralized on the average than dentin or enamel; addition of ACP to the materials did not inhibit demineralization or enhance remineralization of bovine enamel in a pH-cycling as fluoride ions. It was possible to conclude that XMT can determine small alterations in the mineralization of bovine tooth after pH-cycling.

Orientador: Alberto Carlos Botazzo Delbem / Banca: Robson Frederico Cunha / Banca: Cássia Cilene Dezan Garbelini / Banca: Cínthia Pereira Machado Tabchoury / Banca: Célio Percinoto / Resumo: O presente trabalho avaliou a capacidade da microtomografia de raios-X (XMT) laboratorial e síncrotron para mensurar coeficientes de absorção linear em escala micrométrica em estudos in vitro utilizando dentes bovinos e ciclagem de pH. Sendo assim, este estudo foi desenvolvido em cinco manuscritos, com os seguintes objetivos: traçar parâmetros e determinar um protocolo para a quantificação do conteúdo mineral de esmalte bovino utilizando XMT laboratorial e síncrotron; analisar a estrutura da junção amelo-dentinária em incisivos bovinos utilizando XMT síncrotron; determinar o potencial anti-cárie do fosfato de cálcio amorfo (ACP) presente em selantes de fóssulas e fissuras, utilizando dentes bovinos, ciclagem de pH, microdureza de superfície e XMT síncrotron; e avaliar, através de análise de microdureza de superfície e XMT síncrotron, a capacidade de uma pasta contendo CPP-ACP para ativar a remineralização do esmalte dental. Os resultados obtidos mostram que: a XMT propiciou a determinação da quantidade mineral com uma precisão mais adequada, sendo a melhor resolução espacial obtida através da luz síncrotron; a junção amelo-dentinária apresentou-se menos mineralizada que a dentina ou esmalte; o fosfato de cálcio amorfo presente nos materiais testados não foi capaz de inibir a desmineralização ou ativar a remineralização do esmalte bovino, em ciclagem de pH, tanto quanto os íons flúor. Conclui-se que a XMT é capaz de determinar pequenas alterações na mineralização do dente bovino após ciclagem de pH. / Abstract: This study evaluated the ability of laboratory and synchrotron X-ray microtomography (XMT) for measuring linear attenuation coefficient at micrometer scale of in vitro studies using bovine teeth and pH-cycling. For that, this study included five papers, with the following aims: to define parameters and a protocol for quantification of mineral content of bovine enamel using laboratory and synchrotron XMT; to analyze the dentinoenamel junction (DEJ) structure in bovine incisors using synchrotron XMT; to determine the anti-caries potential of amorphous calcium phosphate (ACP) in pit and fissure sealants, using bovine teeth, pH-cycling, surface hardness and synchrotron XMT; and to evaluate, through surface hardness analysis and synchrotron XMT, the ability of a CPP-ACP paste to enhance enamel remineralization. The results showed that: XMT provided mineral quantification with an adequate precision; synchrotron microtomography provided the best spatial resolution; DEJ was less mineralized on the average than dentin or enamel; addition of ACP to the materials did not inhibit demineralization or enhance remineralization of bovine enamel in a pH-cycling as fluoride ions. It was possible to conclude that XMT can determine small alterations in the mineralization of bovine tooth after pH-cycling. / Doutor

Indiana University-Purdue University Indianapolis (IUPUI) / This clinical study investigated the efficacy of the new LED LCU technology when compared to that of the QTH LCU by evaluating retention and wear of Clinpro (3M ESPE) sealant material over six months of function. This study was designed as a split mouth, randomized clinical study. Sealants were placed and polymerized on contralateral teeth of 35 patients, 33 of which successfully completed the study. The sealants were evaluated for clinical retention at baseline, three months, and six months by two evaluators. For the wear analysis, the area of the sealant wear at six months is reported. Nine pairs of molars and 22 pairs of premolar teeth were used. This sample size is smaller than the original sample used for clinical evaluation, because a number of the baseline impressions had to be discarded due to poor impression quality. Subsequent impressions were taken at three months, and six months. Epoxy replicas were made from the impressions and the occlusal surface of each replica was digitized using SigmaScan software. A cummulative legit model was applied to the clinical data, and a linear model was applied to the wear analysis. The results for clinical retention over the six months of function were as follows. At Baseline, for the QTH, 97.3 percent of the teeth received an Alpha score; 2.7 percent received a score of B. For the LED, 87.7 percent received a score of A; 12.3 percent received a score of B. At three months follow-up, for the QTH, 93.1 percent received a score of A; 6.9 percent received a score of B. For the LED, 86.1 percent received a score of A; 12.5 percent received a score of B, and 1.14 percent received a score of C. At six months follow-up, for QTH; 91.7 percent received a score of A; 8.3 percent received a score of B. For the LED, 83.3 percent received a score of A; 15.3 percent received a score of B, and 1.14 percent received a score of C. The hypothesis was that there would be no significant difference in clinical retention and wear of Clinpro's sealant polymerized with the QTH or the LED light sources over six months of function. Based on the results of this clinical study, the following conclusions can be made: 1) At baseline, Clinpro's sealant polymerized with QTH light source showed marginally significant better retention than LED light source (p-value 0.05001). 2) There was no significant difference between light sources for sealant clinical retention at three-month and six-month follow up visits. 3) Wear analysis resulted in marginally significant more wear for molar sealants polymerized with LED LCU (p-value 0.0755). 4) Wear analysis showed no significant difference for premolar sealants polymerized with either light source.

Indiana University-Purdue University Indianapolis (IUPUI) / While most sealants available are resin-based, glass ionomer-based cements can be used as sealants, with the advantage of being more tolerant to moisture during placement and of releasing fluoride. The objective of this study was to evaluate the influence of different fissure conditioning techniques on penetration and microleakage of glass ionomer (GI) and resin-modified glass ionomer cements (RMGI) used as sealants. Clinically sound extracted human molars were distributed into nine experimental groups (n = 15 each). Group 1 (control) was sealed with resin-based sealant (Delton) following clinically accepted techniques. Groups 2 through 6 were sealed with RMGI (Vitremer) after having the fissure conditioned with either polyacrylic acid (RMGI-control), 35-percent H3PO4, low viscosity 35-percent H3PO4 with a surfactant, self-etch conditioner, or 35-percent H3PO4 followed by self-etch conditioner. Groups 7 through 9 were sealed with GI sealant (Fuji Triage) after having the fissures conditioned with either polyacrylic acid (GI-control), 35- percent H3PO4 or low viscosity 35-percent H3PO4 with a surfactant. After aging through thermocycling (2500 cycles), specimens were incubated in methylene blue for four hours and sectioned at multiple locations. Digital images were obtained using a digital stereomicroscope, and microleakage was determined by scoring the dye penetration along the enamel-sealant interface. The penetration of the material was determined by calculating the percentage of the total length of the fissure penetrated by the material. Results: The use of self etch-conditioner significantly increased RMGI penetration, while surface conditioning with 35-percent phosphoric acid with surfactant significantly decreased microleakage of GI. The resin-based sealant placed after 35-percent phosphoric acid surface conditioning showed the best penetration and the least level of microleakage. In conclusion, results from this study suggest that the placement of glass ionomer-based sealants can be enhanced by modifying current conditioning methods.

Este estudo procurou verificar o efeito de dois selantes no tratamento de lesões cariosas não cavitadas em dentina, no que diz respeito à progressão da cárie na superfície oclusal de molares permanentes, em adolescentes com idades entre 11 e 15 anos. A amostra do estudo constituiu-se de 28 dentes: 14 selados com selante resinoso (Fluroshield®, Denstply), no grupo Gres, e 14 selados com selante ionomérico (RIVA Protect®, SDI), no grupo Gciv. A avaliação dos desfechos se deu por meio da análise dos exames radiográficos inicial e após 12 meses de acompanhamento; através de dois métodos: avaliação radiográfica qualitativa e quantitativa. A análise qualitativa foi realizada por um único examinador devidamente calibrado (Kappa = 0,837) com o uso de negatoscópio em sala escura e lupa de aumento 10x, e considerou como sucesso do tratamento a regressão ou paralisação da lesão; e insucesso, a evolução da lesão cariosa. A análise quantitativa se deu por meio da comparação entre os coeficientes de densidade radiográfica obtidos através do software DIGORA®, observando aumento (sucesso) ou diminuição (insucesso) dos coeficientes de densidade. Após a avaliação qualitativa, observou-se sucesso do tratamento em 64,3% dos casos (n=9) do grupo Gciv e 85,7% (n=12) do grupo Gres, sem diferença estatística entre os grupos (p=0,39). A avaliação quantitativa mostrou que no Gres foi observado um aumento significante dos coeficientes de densidade (p=0,003) e no Gciv se observou uma estabilidade dos coeficientes após 12 meses (p=0,49). Desta forma, pode-se concluir que, nas condições deste estudo, o uso de selantes de sulcos e fóssulas mostrou-se eficaz no tratamento de lesões cariosas. / This study examined the effect of two sealants in the treatment of non-cavitated occlusal carious lesions, regarding to the progression or arresting of the lesions in permanent molars in adolescents 11 to 15 years age. The sample consisted of 28 teeth: 14 sealed with resin-based sealant (Fluroshield®, Denstply), Gres group, and 14 sealed with glass ionomer sealant (RIVA Protect®, SDI), Gciv group. Caries progression was monitored by radiographic examination, using two methods: qualitative and quantitative trough radiographic evaluation. Qualitative analysis was performed by a single calibrated examiner (Kappa = 0.837) using light box in a darkened room and 10x magnifying glass, and considered as success treatment the regression or interruption of the injury caries, and failure treatment, the evolution of the lesion carious. Quantitative analysis occurred by comparing the radiographic density coefficient obtained through software DIGORA®, observing increased (success) or decreased (failure) of the coefficients. After the qualitative evaluation, it was observed success of treatment in 64.3% (n = 9) of Gciv group and 85.7% (n = 12) on Gres group, with no statistical difference between the them (p=0,39). The quantitative analysis showed in Gres group a significant increase in the density coefficient (p = 0.003) and in the Gciv group the stability of coefficients after 12 months (p = 0.49). It can be concluded that under the conditions of this study, the use of sealants proved to be effective in the treatment of occlusal carious lesions.