• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 43
  • 6
  • 4
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • Tagged with
  • 63
  • 63
  • 7
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
31

Induction of microspore embryogenesis in Brassica Napus Via anther culture and isolated microspore culture.

January 1991 (has links) (PDF)
Thesis (M.Ag.Sc.) -- University of Adelaide, Dept. of Plant Science, 1991. / Includes bibliographical references (p. 230-281).
32

The role of auxin on the evolution of embryo development and axis formation in land plants /

Poli, Dorothy Belle. January 2005 (has links)
Thesis (Ph. D.) -- University of Maryland, College Park, 2005. / Title from caption. Downloaded and printed on June 9, 2009. Includes bibliographical references ([152-199]). Also available online.
33

A study of the factors affecting the development of the embryo-sac and the embryo in the McIntosh apple

Bryant, Louis Ralph, January 1935 (has links)
Thesis (Ph. D.)--University of Illinois, 1934. / Vita. Bibliography: p. 39-40.
34

On the cytology and embryology of Smilacina racemosa

McAllister, Frederick. January 1913 (has links)
Presented as Thesis (Ph. D.)--University of Wisconsin--Madison, 1910. / Cover title. Reprinted from Transactions of the Wisconsin Academy of Sciences, Arts and Letters, vol. XVII, Part I. eContent provider-neutral record in process. Description based on print version record. "Literature cited": p. 647-655.
35

An ultrastructural study of megasporogenesis in oryza sativa, cymbidium sinense and phaius tankervilliae /

Tung, Shui-hoi. January 1997 (has links)
Thesis (M. Phil.)--University of Hong Kong, 1997. / Includes bibliographical references (leaf 72-83).
36

The respiratory metabolism of germinating Abutilon theophrasti seed

Mulliken, Jerry Alan, January 1970 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1970. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
37

Über die Embryonen pharmakognostisch wichtiger Samen

Ruben, Martin, January 1918 (has links)
Thesis (doctoral)--Universität Halle-Wittenberg, 1918. / Cover title. Includes bibliographical references.
38

In vitro studies on the embryo of Capsella bursa-pastoris

Rijven, Antonius Henricus Gerardus Cornelius. January 1952 (has links)
Proefschrift--Utrecht. / Bibliography: p. 198-200.
39

Studien über konstruktonstypen und entwicklungswege des embryosackes der angiospermen

Palm, Björn Torvald, January 1915 (has links)
Inaug.-diss.--Hochschule, Stockholm / "Litteraturverzelchnis" : p. 243-259.
40

Promoter analysis and endosperm-specific expression of rice phytoene synthase genes (psy1 and psy2) in rice.

January 2011 (has links)
Leung, Chiu Yi. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2011. / Includes bibliographical references (leaves 219-235). / Abstracts in English and Chinese. / ACKNOWLEDGEMENTS --- p.iii / ABSTRACT --- p.v / 摘要 --- p.vii / TABLE OF CONTENTS --- p.ix / LIST OF FIGURES --- p.xiv / LIST OF TABLES --- p.xviii / LIST OF ABBREVIATIONS --- p.xix / Chapter CHAPTER 1. --- GENERAL INTRODUCTION --- p.1 / Chapter CHAPTER 2. --- LITERATURE REVIEW --- p.5 / Chapter 2.1 --- Introduction to carotenoids --- p.5 / Chapter 2.1.1 --- Structures and general chemical properties --- p.6 / Chapter 2.1.2 --- Dietary source of carotenoids --- p.8 / Chapter 2.2 --- Biosynthesis of carotenoids in plants --- p.8 / Chapter 2.2.1 --- Formation of isopretenyl diphosphate (IPP) --- p.12 / Chapter 2.2.2 --- "Formation of C40 backbone, phytoene" --- p.13 / Chapter 2.2.3 --- Desaturation reactions --- p.16 / Chapter 2.2.4 --- Isomerization --- p.18 / Chapter 2.2.5 --- Cyclization --- p.18 / Chapter 2.2.6 --- Xanthophylls synthesis --- p.19 / Chapter 2.2.6.1 --- Hydroxylation --- p.19 / Chapter 2.2.6.2 --- Epoxidation and de-epoxidation --- p.19 / Chapter 2.2.6.3 --- Neoxanthin formation --- p.20 / Chapter 2.2.7 --- Carotenoids catabolism by cleavage enzymes --- p.20 / Chapter 2.2.8 --- Carotenoids sequestration --- p.20 / Chapter 2.2.9 --- Regulations of Carotenogenesis in plant --- p.21 / Chapter 2.3 --- Roles of carotenoids in plants --- p.24 / Chapter 2.3.1 --- Precursor of abscisic acid (ABA) production --- p.24 / Chapter 2.3.2 --- Photomorphogenesis: Prolamella body formation --- p.27 / Chapter 2.3.3 --- "Light-harvesting, energy transfer and photoprotection" --- p.30 / Chapter 2.4 --- Importance of carotenoids to human --- p.34 / Chapter 2.4.1 --- Provitamin A activity and conversion --- p.34 / Chapter 2.4.2 --- Roles of vitamin A and carotenoids in diseases prevention --- p.36 / Chapter 2.4.2.1 --- Visual cycle and related diseases --- p.36 / Chapter 2.4.2.2 --- Cardiovascular diseases --- p.37 / Chapter 2.4.2.3 --- Cancer --- p.38 / Chapter 2.4.3 --- Roles of vitamin A in gene regulation --- p.39 / Chapter 2.4.4 --- Bioavailability and daily intake recommendation --- p.39 / Chapter 2.5 --- Vitamin A deficiency (VAD) --- p.42 / Chapter 2.5.1 --- Clinicopathological features --- p.44 / Chapter 2.5.1.1 --- Visual problems --- p.44 / Chapter 2.5.1.2 --- Infection --- p.45 / Chapter 2.6 --- Global efforts in combating VAD --- p.45 / Chapter 2.6.1 --- Dietary diversification --- p.46 / Chapter 2.6.2 --- Supplementation --- p.48 / Chapter 2.6.3 --- Pro-vitamin A enriched crops by genetical engineering --- p.49 / Chapter 2.6.3.1 --- Tomato --- p.49 / Chapter 2.6.3.2 --- Potato --- p.50 / Chapter 2.6.3.3 --- Canola --- p.50 / Chapter 2.6.3.4 --- The Golden Rice (GR) project --- p.51 / Chapter 2.6.3.4.1 --- The 1st generation (GR1) --- p.52 / Chapter 2.6.3.4.2 --- The 2nd generation (GR2) --- p.54 / Chapter 2.7 --- Rice phytoene synthase as a GR candidate enzyme --- p.55 / Chapter 2.7.1 --- General properties of phytoene synthase in higher plant --- p.55 / Chapter 2.7.1.1 --- Gene duplication and structure --- p.55 / Chapter 2.7.1.2 --- Membrane association and cation requirement --- p.57 / Chapter 2.7.1.3 --- Expression pattern and tissue-specificity --- p.58 / Chapter 2.7.2 --- "Rice phytoene synthases: Ospsy1, Ospsy2 and Ospsy3" --- p.60 / Chapter 2.7.2.1 --- Gene duplication and structure --- p.60 / Chapter 2.7.2.2 --- Protein structures and phylogenetic analysis --- p.60 / Chapter 2.7.2.3 --- Expression pattern --- p.62 / Chapter 2.7.2.4 --- Light- and stress-induced expression --- p.63 / Chapter 2.7.3 --- Rice phytoene synthases activity in rice seeds --- p.64 / Chapter 2.7.3.1 --- Previous study in rice seed carotenogenic capacity --- p.64 / Chapter 2.8 --- Seed-specific rice promoters --- p.66 / Chapter 2.8.1 --- Previous studies on seed-specific expression in rice --- p.66 / Chapter 2.8.1.1 --- Endosperm-specific cis-acting regulatory elements --- p.67 / Chapter 2.8.1.2 --- Requirements to confer endosperm-specific expression in rice --- p.69 / Chapter 2.9 --- Project overview and hypothesis --- p.71 / Chapter CHAPTER 3. --- MATERIALS AND METHODS --- p.73 / Chapter 3.1 --- Chemicals --- p.73 / Chapter 3.2 --- Vectors and bacterial strains in regular cloning --- p.73 / Chapter 3.3 --- Plant materials --- p.74 / Chapter 3.4 --- Construction of gene cassettes for plant transformation --- p.74 / Chapter 3.4.1 --- Construction of gene cassettes for Ospsyl and Ospsy2 study --- p.74 / Chapter 3.4.1.1 --- Cloning of Ospsyl and Ospsy2 from rice --- p.76 / Chapter 4.1.1.1 --- Total RNA extraction --- p.75 / Chapter 3.4.1.1.2 --- Amplification of cDNA of Ospsyl by RT-PCR --- p.77 / Chapter 3.4.1.1.3 --- Amplification of cDNA of Ospsy2 by PCR --- p.80 / Chapter 3.4.1.2 --- Cloning of constitutive CaMV35S promoter --- p.82 / Chapter 3.4.1.2.1 --- Preparation of pBI121 vector --- p.82 / Chapter 3.4.1.2.2 --- Amplification of CaMV35S promoter by PCR --- p.82 / Chapter 3.4.1.3 --- Cloning of endosperm-specific rice glutelin-1 (Gt-1) promoter --- p.84 / Chapter 3. 4.1.3.1 --- Genomic DNA extraction --- p.84 / Chapter 3.4.1.3.2 --- Amplification of Gt-1 promoter by PCR --- p.84 / Chapter 3.4.1.4 --- Construction of gene cassettes for Ospsyl and Ospsy2 driven by CaMV35S and rice Gt-1 promoter --- p.87 / Chapter 3.4.2 --- Construction of gene cassettes for promoter analysis --- p.90 / Chapter 3.4.2.1 --- Cloning of full length and fragments of Ospsyl promoter --- p.92 / Chapter 3.4.2.1.1 --- Genomic DNA extraction --- p.95 / Chapter 3.4.2.1.2 --- Amplification of full length and fragments of Ospsy1 promoter --- p.95 / Chapter 3.4.2.2 --- Cloning of CaMV35S minimal promoter --- p.96 / Chapter 3.4.2.2.1 --- Amplification of CaMV35S minimal promoter --- p.97 / Chapter 3.4.2.3 --- Cloning of rice Gt-1 promoter --- p.97 / Chapter 3.4.2.3.1 --- Genomic DNA extraction --- p.97 / Chapter 3.4.2.3.2 --- Amplification of Gt-1 promoter --- p.97 / Chapter 3.4.2.4 --- Annealing of linker --- p.93 / Chapter 3.4.3.5 --- Making of rice GCN4 multimers --- p.98 / Chapter 3.4.2.6 --- Construction of gene cassettes for promoter analysis --- p.99 / Chapter 3.4.2.7 --- Construction of gene cassettes for Gt-1 promoter analysis --- p.104 / Chapter 3.4.3 --- Confirmation of sequence fidelity --- p.106 / Chapter 3.5 --- Rice transformation --- p.107 / Chapter 3.5.1 --- Plant materials --- p.107 / Chapter 3.5.2 --- Preparation of Agrobacterium --- p.107 / Chapter 3.5.3 --- Agrobacterium mediated transformation --- p.108 / Chapter 3.5.4 --- Callus induction from mature rice seeds --- p.109 / Chapter 3.5.6 --- Co-cultivation and selection --- p.109 / Chapter 3.5.7 --- Pre-regeneration and regeneration of transgenic rice --- p.110 / Chapter 3.5.8 --- Plantation of transgenic rice --- p.110 / Chapter 3.6 --- Detection of transgene expression --- p.112 / Chapter 3.6.1 --- Detection at DNA level --- p.112 / Chapter 3.6.1.1 --- Genomic DNA extraction --- p.112 / Chapter 3.6.1.2 --- PCR screening --- p.122 / Chapter 3.6.1.3 --- Synthesis of DIG-labeled DNA probes --- p.116 / Chapter 3.6.1.4 --- Southern blot analysis --- p.118 / Chapter 3.6.2 --- Detection at RNA level --- p.119 / Chapter 3.6.2.1 --- Total RNA extraction --- p.119 / Chapter 3.6.2.2 --- Northern blot analysis --- p.119 / Chapter 3.6.3 --- Detection at protein level --- p.119 / Chapter 3.6.3.1 --- Antibody production --- p.119 / Chapter 3.6.3.1 --- Ospsyl and Ospsy2 induction in pET system --- p.120 / Chapter 3.63.1.2 --- Immunization of rabbit and serum collection --- p.123 / Chapter 3.6.3.2 --- Total protein extraction from plant materials --- p.124 / Chapter 3.6.3.2.1 --- Protein extraction from rice calli and leaves --- p.124 / Chapter 3.6.3.2.2 --- Protein extraction from immature and mature rice seeds --- p.124 / Chapter 3.6.3.3 --- Tricine SDS-PAGE --- p.125 / Chapter 3.6.3.4 --- Western blot analysis --- p.125 / Chapter 3.6.4 --- Detection at metabolite level --- p.126 / Chapter 3.6.4.1 --- Isoprenoids extraction from plant materials --- p.126 / Chapter 3.6.4.2 --- UPLC analysis for isoprenoid identification --- p.127 / Chapter 3.6.5 --- Detection of promoter activity --- p.128 / Chapter 3.6.5.1 --- Histochemical staining of GUS activity --- p.128 / Chapter 3.6.5.1.1 --- Histochemical staining --- p.128 / Chapter 3.6.5.1.2 --- Plant tissue fixation for microscopic observation --- p.128 / Chapter 3.6.5.2 --- GUS activity assay --- p.129 / Chapter 3.6.5.2.1 --- Protein extraction and quantitation with Bio-Rad protein assay --- p.129 / Chapter 3.6.5.2.2 --- G US activity assay --- p.130 / Chapter CHAPTER 4. --- RESULTS --- p.131 / Chapter 4.1 --- Tissue-specificity and endosperm specific expression of rice psy1 and Psy2 --- p.131 / Chapter 4.1.1 --- Construction of gene cassettes for study in Ospsy1 and Ospsy2 --- p.133 / Chapter 4.1.2 --- Rice transformation --- p.135 / Chapter 4.1.3 --- Transgene detection --- p.137 / Chapter 4.1.3.1 --- Genomic DNA PCR screening --- p.137 / Chapter 4.1.3.2 --- Southern blot analysis --- p.139 / Chapter 4.1.3.2.1 --- Southern blot analysis on transgenic rice calli --- p.141 / Chapter 4.1.3.2.2 --- Southern blot analysis on regenerated rice --- p.143 / Chapter 4.1.4 --- Detection of transgene expression --- p.149 / Chapter 4.1.4.1 --- Northern blot analysis on immature transgenic seed --- p.149 / Chapter 4.1.4.2 --- Western blot analysis on transgenic rice tissues --- p.152 / Chapter 4.1.4.2.1 --- Antibody production --- p.152 / Chapter 4.1.4.2.2 --- Western blot analysis of transgenic rice calli --- p.155 / Chapter 4.1.4.2.3 --- Western blot analysis of transgenic rice leaves --- p.157 / Chapter 4.1.4.2.4 --- Western blot analysis of immature transgenic rice seeds --- p.160 / Chapter 4.1.5 --- Detection of OsPSYs activity at metabolite level --- p.163 / Chapter 4.1.5.1 --- UPLC analysis on transgenic rice tissues --- p.163 / Chapter 4.1.5.1.1 --- Carotenoid profiling of transgenic rice calli --- p.163 / Chapter 4.1.5.1.2 --- Carotenoid profiling of transgenic rice leaves --- p.168 / Chapter "4.1.5.1,3" --- Carotenoid profiling of mature transgenic rice seeds --- p.172 / Chapter 4.2 --- Promoter analysis of modified rice psy1 promoter --- p.176 / Chapter 4.2.1 --- Construction of gene cassettes for promoter analysis --- p.178 / Chapter 4.2.2 --- Rice transformation --- p.180 / Chapter 4.2.3 --- Transgene detection --- p.180 / Chapter 4.2.3.1 --- Genomic DNA PCR screening --- p.180 / Chapter 4.2.3.2 --- Southern blot analysis --- p.185 / Chapter 4.2.3.2.1 --- Southern blot analysis of regenerated rice --- p.186 / Chapter 4.2.4 --- Detection of promoter activity --- p.196 / Chapter 4.2.4.1 --- Promoter activity in transgenic rice leaves --- p.196 / Chapter 4.2.4.1.1 --- Histochemical staining of GUS --- p.196 / Chapter 4.2.4.1.2 --- G US activity assay --- p.199 / Chapter 4.2.4.2 --- Promoter activity in transgenic immature seeds --- p.202 / Chapter 4.2.4.2.1 --- Histochemical staining of GUS --- p.202 / Chapter 4.2.4.2.2 --- GUS activity assay --- p.206 / Chapter CHAPTER 5. --- DISCUSSIONS --- p.209 / Chapter 5.1 --- Tissue-specificity and endosperm specific expression of rice psyl and psy2 --- p.209 / Chapter 5.1.1 --- OsPSYl and OsPSY2 activities in rice calli --- p.209 / Chapter 5.1.2 --- OsPSYl and OsPSY2 activities in rice leaves --- p.210 / Chapter 5.1.3 --- OsPSYl and OsPSY2 activities in rice seeds --- p.211 / Chapter 5.2 --- Analysis of modified rice psyl promoter --- p.213 / Chapter 5.3 --- Future prospects of Golden Rice --- p.214 / Chapter CHAPTER 6. --- CONCLUSIONS --- p.217 / REFERENCES --- p.219

Page generated in 0.0844 seconds