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Many insects form close relationships with microbial symbionts. Insect symbionts can provide novel phenotypes to their hosts, including influencing dietary breadth. In the polyphagous cowpea aphid, Aphis craccivora, the facultative symbiont Arsenophonus improves aphid performance on one host plant (locust), but decreases performance on other plants. The goal of my thesis was to investigate the mechanism by which Arsenophonus facilitates use of locust. First, I assembled an Aphis craccivora-Arsenophonus-Buchnera reference transcriptome to conduct RNAseq analysis, comparing gene expression in aphids feeding on locust and fava, with and without Arsenophonus infection. Overall, few transcripts were differentially expressed. However, genes that were differentially expressed mapped to a variety of processes, including metabolism of glucose, cytoskeleton regulation, cold and drought regulation, and B-vitamin synthesis. These results imply that Arsenophonus is producing B-vitamins, which might be deficient in locust. In a second set of experiments, I used qPCR to test whether symbiont function across host plants might be mediated by bacterial titer. I measured relative Arsenophonus abundance across plants, and found Arsenophonus titer was variable, but generally greater on locust than fava. In summary, my results suggest that Arsenophonus synthesis of B-vitamins should be further investigated and may be mediated by bacterial titer.

<p>Due to overpopulation and resource-poor habitat structure, deer threaten the<a> future of oak and other browse-sensitive species in hardwood forests. </a>Appropriate tools must be used to ensure desirable, diverse, and ecologically stable regeneration of future forests and the sustainability of native plant communities. We performed two experiments and a review to examine the effectiveness of available methods for managing browse of hardwood seedlings and to discover how these interact with each other and other silvicultural methods. First, we examined how fencing interacts with controlled-release fertilization, seed source (genetically select and non-select), and site type (afforested and reforested sites) to enhance the regeneration of planted northern red oak (<i>Quercus rubra </i>L.), white oak (<i>Quercus alba</i>), black cherry (<i>Prunus serotina</i>), and black walnut (<i>Juglans nigra</i>) at five sites in Indiana. Fencing proved to be the greatest determinant of seedling growth, survival, and quality. Fertilizer enhanced the early growth of white oak and black cherry, though for black cherry this occurred only inside fences. Select seed sources grew better and showed greater quality; however, the survival of select seedlings was limited by deer browse in absence of fences. Trees at afforested sites had lower survival if left non-fenced. Secondly, we also investigated how fencing and invasive shrub removal affected natural regeneration, species richness, and ground-layer plant cover under closed-canopy forests. Honeysuckle (<i>Lonicera maackii</i>) removal had a variable effect depending on species and site. Positive effects were most common for shade-intolerant species, while negative effects occurred for a few shade-tolerant species at some sites. Deer fencing had a positive effect on cherry and hackberry seedling density, and a negative effect on elm seedling density. Honeysuckle and deer fencing interacted antagonistically in some instances. Fencing without honeysuckle removal resulted in lower elm abundance and herbaceous-layer cover. In the densest invasions, leaving honeysuckle intact resulted in a complete lack of recruitment into the sapling layer. Our experiment suggests that invasive shrub removal and fencing be done together. Finally, we synthesized the existing literature on browse management options for hardwood regeneration to evaluate their relative effectiveness. Fences, tree shelters, repellents, facilitation by neighboring plants, deer population control, timber harvest, and slash all had positive effects on height growth of regenerating seedlings under deer browse pressure. Fences were more effective at reducing browse than repellents, while fertilizers increased browse and had no effects on growth. </p>