Currently the breeding of desirable traits in orchid flowers is a lengthy and unpredictable process. A shortened breeding time and a more direct method of introducing specific genetic characteristics could be achieved if more information were available on the specific genes responsible for flower characteristics. In order to identify some of these genes, the genetic relationships between a hybrid, Dendrobium Gatton 'Sunray', and the parent species bred to produce it, D. chrysotoxum Lindley and D. pu/che//um Lindley were examined.Ball State UniversityMuncie, IN 47306These results were supported by Restriction Fragment Length Polymorphisms (RFLPs) observed following amplification of the Internal Transcribed Spacer (ITS) regions of the rDNAs.In order to clone genes responsible for specific flower characteristics, mRNA differential display was performed using total RNA isolated from the leaves, immature flowers, and mature flowers of the hybrid orchid and its two parents. Bands unique to D. Gatton 'Sunray' flower tissue, which were common to the hybrid and a single parent, were excised from a denaturing acrylamide gel. Four of the bands, which represented expressed genes determining inherited flower characteristics, were re-amplified, cloned, and three were sequenced. Partial sequence information obtained for two of the clones was used to search the GenBank database for homologous genes. One of the clones had sequence homology to plant 26S ribosomal genes and the other clone was homologous to sequences encoding regulatory proteins active during development (for example, the human retinoblastoma susceptibility gene or the Caenorhabditis e/egans cosmid R06F6 containing a serine/threonine protein kinase gene). / Department of Biology

Thesis (Ph. D.)--University of Oregon, 2007. / Typescript. Includes vita and abstract. Includes bibliographical references (leaves 201-241). Also available for download via the World Wide Web; free to University of Oregon users.

Thesis (Ph. D.)--University of Adelaide, Dept of Horticulture, Viticulture and Oenology, 1997. / Copy of author's previously presented paper inserted. Includes bibliographical references (leaves 187-218).

Thesis (Ph.D. 1979) -- University of Adelaide, Dept. of Agronomy, 1978.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. [118]-122.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xx, 305 p.; also includes graphics. Includes bibliographical references (p. 278-305). Available online via OhioLINK's ETD Center

Over one billion people, more than 1/9th of the global population, are undernourished. Feeding the ever increasing population has to be the most important goal of plant sciences. Since cultivated areas are not likely to increase, I will need to produce more with what is available. This can be summarized in one word: yield. Unfortunately, wheat’s yield is expected to increase only 1.13% by 2019, a prediction that if converted into reality will likely indicate that I failed to cope with the world demographic increase. A new strategy to revolutionize wheat production is required, and some believe that this change might be represented by wheat hybrids. Achieving adequate commercial production of wheat hybrids has the potential to nearly double the yield of one of the world’s most important staple food. The first fundamental step toward this goal is to develop feasible methodologies to sterilize the male part of the complete wheat flowers. Two fertility-related genes are the primary target of this study, namely the species cytoplasm specific on chromosome 1D, and the desynaptic locus on chromosome 3B. This dissertation summarizes the important achievements obtained toward the cloning of the two loci by means of radiation hybrid functional analysis. Radiation hybrid is a technique that employs radiation to create genetic diversity along the targeted chromosome. Chapter 1 explains in details how this methodology can be applied to plants. The use of radiation hybrid mapping permitted creating a comprehensive map of wheat chromosome 3B, as discussed in Chapter 2, and then expanded the mapping information to identify the 2 Mb location of the desynaptic locus desw2, as discussed in Chapter 3. A similar approach on chromosome 1D allowed first to pinpoint the location of the species cytoplasm specific gene to a region of 2 Mb, as discussed in Chapter 4, and then ultimately to find a strong candidate for this locus, as discussed in Chapter 5. Now that the molecular locations of these genes have been unraveled by this study, their sequence can be streamlined into transformation to ultimately produce female wheat plants, and consequently hybrids.

No description available.

Master of Science

O presente estudo teve como objetivo principal avaliar o potencial da madeira de dois clones do híbrido Eucalyptus grandis x Eucalyptus urophylla para a produção de lâminas e manufatura de painéis compensados. Foram coletadas 5 árvores para cada clone (I e II) e duas toras de cada árvore, sendo uma da base (A) e outra subseqüente (B), totalizando 20 toras. As toras após descascamento e aquecimento em água quente foram processadas em torno laminador gerando lâminas de 2,00 mm de espessura nominal. O rendimento médio do processo de laminação foi de 51,74% para as toras do clone I e de 56,81% para as toras do clone II. A qualidade das lâminas produzidas foi avaliada com base na Norma de Controle de Qualidade e Classificação de Compensados, da ABNT. O clone I gerou lâminas de melhor qualidade: 11,94 % na classe A; 32,84 % na B; 54,48 % na C e 0,75 % na classe D, enquanto que o clone II gerou: 1,20 % na classe A; 8,40 % na B; 67,47 % na C e 22,89 % na classe D. Foram manufaturados compensados de 5 camadas, com lâminas da classe C para as capas e o miolo, coladas com adesivo à base de uréia-formaldeído. Os fatores de tratamento empregados na manufatura foram: clone (I e II), gramatura de cola (320 e 360 g/m2) e tempo de prensagem (8 e 12 minutos). O delineamento experimental foi inteiramente casualizado com arranjo fatorial do tipo 2 x 2 x 2. A partir dos compensados manufaturados foram obtidos corpos de prova para avaliação das propriedades físicas - massa específica aparente, absorção superficial de água, absorção total de água, inchamento mais recuperação em espessura e recuperação em espessura, e avaliação das propriedades mecânicas - flexão estática paralela e perpendicular (MOR e MOE) e resistência da linha de colagem ao esforço do cisalhamento nas condições seca e úmida. A análise estatística dos resultados das propriedades físicas e mecânicas foi realizada através de uma análise de variância. Não houve interação significativa entre os fatores clone, gramatura de cola e tempo de prensagem, considerando todas as variáveis avaliadas. Foram detectadas diferenças significativas para o fator clone em relação às variáveis massa específica aparente (maior para o clone I), absorção superficial e total de água (menores para o clone I); resistência da linha de colagem ao esforço do cisalhamento - condição seca e flexão estática - MOR paralelo e perpendicular (maiores para o clone I). Para o fator gramatura de cola não houve diferença significativa entre os tratamentos para nenhuma das variáveis avaliadas. Houve diferença significativa para o fator tempo de prensagem em relação às variáveis absorção superficial de água (menor para o tempo de prensagem de 12 minutos), inchamento mais recuperação em espessura e recuperação em espessura (menores para o tempo de prensagem de 8 minutos). A conclusão do trabalho é que as madeiras de ambos clones do híbrido Eucalyptus grandis x Eucalyptus urophylla apresentam alto potencial para produção de lâminas e manufatura de compensados. / The main objective of this work was to evaluate the potencial of eucalyptus grandis x Eucalyptus urophylla hybrid clones wood for plywood and veneer production. Five (5) trees were collected for each clone (I and II) and 2 logs of each tree, being one from base (A) and other subsequent one (B), totaling 20 logs. The logs after debarked and heated in hot water were processed in rotary cutting lathe generating veneers of 2,00 mm nominal thickness. The peeling yield was evaluated and mean values were 51,74% from clone I logs and 56,81% from the II one. The veneer quality graded was conduced following Norma de Controle de Qualidade e Classificação de Compensados, Brazilians standards. The clone I generated veneers of better quality: 11,94% of grade A veneer; 32,84% of B; 54,48% of C and 0,75% of grade D veneer, while the clone II generated: 1,20% of grade A veneer; 8,40% of B; 67,47% of C and 22,89% of grade D veneer. 5 layers plywood was manufactured with grade C veneers. The plywood manufacture treatment factors were: clone (I and II), glue amount (320 and 360 g/m2) and press time (8 and 12 minutes). From manufacture plywood were obtained specimens to evaluate the physical properties - apparent specific mass, superficial absorption of water, total absorption of water, thickness swelling more thickness recovery and thickness recovery, and the mechanical properties - parallel and perpendicular static bending (MOR and MOE) and bonding line shear test in the dry and wet conditions. The result obtained during the physical and mechanical assessments of plywood were analyzed using a variance analysis, executed by SAS (Statistical Analysis System). There was not significant interaction among the factors clone, glue amount and press time, considering all the appraised variables. Significant differences were detected for factor clone in relation to the variables apparent specific mass (clone I higher than clone II), superficial and total absorption of water (clone I smaller than clone II); bonding line shear test (dry condition) and static bending - parallel and perpendicular MOR (clone I higher than clone II). To the factor glue amount there was not significant difference among the treatments for none of the appraised variables. There was significant difference for the factor press time in relation to the variables superficial absorption of water (12 minutes smaller than 8 minutes), thickness swelling more thickness recovery and thickness recovery (8 minutes smaller than 12 minutes). It's concluded that Eucalyptus grandis x Eucalyptus urophylla hybrid clones wood have a high potencial for phywood and veneer production.