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Bacterial Endophytes from Pioneer Desert Plants for Sustainable AgricultureEida, Abdul Aziz 06 1900 (has links)
One of the major challenges for agricultural research in the 21st century is to increase crop productivity to meet the growing demand for food and feed. Biotic (e.g. plant pathogens) and abiotic stresses (e.g. soil salinity) have detrimental effects on agricultural productivity, with yield losses being as high as 60% for major crops such as barley, corn, potatoes, sorghum, soybean and wheat, especially in semi-arid regions such as Saudi Arabia. Plant growth promoting bacteria isolated from pioneer desert plants could serve as an eco-friendly, sustainable solution for improving plant growth, stress tolerance and health. In this dissertation, culture-independent amplicon sequencing of bacterial communities revealed how native desert plants influence their surrounding bacterial communities in a phylogeny-dependent manner. By culture-dependent isolation of the plant endosphere compartments and a number of bioassays, more than a hundred bacterial isolates with various biochemical properties, such as nutrient acquisition, hormone production and growth under stress conditions were obtained. From this collection, five phylogenetically diverse bacterial strains were able to promote the growth of the model plant Arabidopsis thaliana under salinity stress conditions in a common mechanism of inducing transcriptional changes of tissue-specific ion transporters and lowering Na+/K+ ratios in the shoots. By combining a number of in vitro bioassays, plant phenotyping and volatile-mediated inhibition assays with next-generation sequencing technology, gas chromatography–mass spectrometry and bioinformatics tools, a candidate strain was presented as a multi-stress tolerance promoting bacterium with potential use in agriculture. Since recent research showed the importance of microbial partners for enhancing the growth and health of plants, a review of the different factors influencing plant-associated microbial communities is presented and a framework for the successful application of microbial inoculants in agriculture is proposed. The presented work demonstrates a holistic approach for tackling agricultural challenges using microbial inoculants from desert plants by combining culturomics, phenomics, genomics and transcriptomics. Microbial inoculants are promising tools for studying abiotic stress tolerance mechanisms in plants, and they provide an eco-friendly solution for increasing crop yield in arid and semi-arid regions, especially in light of a dramatically growing human population and detrimental effects of global warming and climate change.
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Developing Production Methods for Different Microbial Strains and Beneficial Testing on Crop SpeciesAlghanmi, Linah Y. 07 1900 (has links)
Microorganisms will play a significant role in the agricultural revolution in the coming decades and help meet the growing population's needs. Hence, understanding the impact of beneficial bacteria on crop development is key to the future of developing microbial products. The ability of PGPB to increase crop yields has been recently investigated in agriculture, as PGPB can support and protect plants under different stresses. Since PGPB interactions occur naturally, finding a method to apply beneficial bacteria while maintaining their efficiency and quality is a topic of interest. PGPB have been used as microbial inoculants, biofertilizers, and also as seed coatings. Preservation of microorganisms through desiccation has been used as the preferred method for long-term storage of microbial culture. The use of dry powders is favored over liquid cultures due to their ease of transportation and better quality control. For microbial preservation, freeze-drying has been defined as the most convenient and satisfactory preservation method for long-term storage. Freeze-drying is generally preferred over other drying techniques as it gives a high-quality dehydrated product. However, to reach a high-quality product, many parameters need to be monitored, such as bacterial cell concentration, growth medium, lyophilization buffer, rehydration, and duration of freeze-drying.
In this research, SA190 was freeze-dried with 10% sucrose mixed with 5% trehalose as lyophilization buffer. Pseudomonas argentinensis SA190 was isolated from the root nodules of the desert plant Indigofera argentae in Saudi Arabia, specifically Jizan. The SA190 freeze-dried product was examined by several tests to assess the product viability and quality, such as accelerated test and water stability test.
For future work, the effect of freeze-dried SA190 on plant growth and crop yield will be investigated. Moreover, optimization of the freeze-drying process, formulation, and packaging for commercial will be considered. In addition, bacterial strains isolated in DARWIN21 project with promising effects on plant growth, will be subjected to freeze-drying process.
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Burkholderia sp. cadmium tolerance mechanism and its influence in phytoremediation / Mecanismos de tolerância ao cádmio em Burkholderia sp. e sua aplicação na fitorremediaçãoRibeiro, Manuella Nóbrega Dourado 22 November 2013 (has links)
Soils have been contaminated with cadmium (Cd) by the use of fertilizers, calcareous, pesticides and industrial and/or domestic effluents. It can be leached to groundwater, as well as be taken up by plants potentially leading to reduce growth and yield. It causes different damages to the cell, generating oxidative stress which is responsible for its toxicity, affecting all living organism. A balance in the redox state of the cell to maintain cellular integrity and metabolism is essential for organism tolerance. Thus, the antioxidant response of bacteria exposed to Cd was studied to understand the tolerance mechanism, and be able to develop a methodology to bioremediate contaminated soils. MDA and hydrogen peroxide contents and different enzymes activity of antioxidant system (SOD, CAT, GR and GST) of two strains from Burkholderia genus, one from a soil contaminated with Cd in high concentrations (strain SCMS54) and the other from soil without Cd (strain SNMS32) in two exposure time (5 and 12 h), were analyzed. Stress measurement (MDA and hydrogen peroxide content) and antioxidant enzyme activities (SOD, CAT, GR and GST) increased in almost all treatments in the presence of Cd. These results also indicate that strain SCMS54 (isolated from Cd contaminated soil) presents a higher metabolic diversity and plasticity due the expression of more isoforms of the enzymes SOD, CAT and GR. The strain also accumulates 50% more Cd. We also analyzed the response to Ni of these two strain, observing a similar response to Cd, except for GST enzyme expression, which in strain SCMS54 this enzyme was induced in the presence of Ni, indicating that this enzyme can be essential on Ni tolerance. After that, the strain isolated from Cd contaminated soil (SCMS54) was selected to proceed the studies to evaluate the benefits of tolerant microorganism-tomato plant interaction. The use of plants to remove heavy metals from contaminated soilhas less impact and a lower cost. Soil microorganisms can be able to solubilize or mobilize soil metals acting also as bioremediator. Besides the high tolerance to Cd, the strain SCMS54 can produce indole-acetic acid (IAA), solubilize inorganic phosphate and produce siderophore, revealing its potential in plantmicroorganism mutual and beneficial interaction. When interacting with tomato plants exposed to Cd, this bacterium led to decrease in plant peroxide concentration and chlorosis levels, promoted relative plant growth and reduced the root absorption of Cd resulting in an increase in plant tolerance to this highly toxic heavy metal. Indicating that inoculation of tomato plants with Burkholderia sp. SCMS54 promotes better growth when cultivated in the presence of Cd by a mechanism that appears to decrease Cd concentration in roots as a result of a bacterial-plant root beneficial interaction. / O cádmio (Cd) tem contaminado solos pelo uso de fertilizantes, calcário, agrotóxicos e resíduos industriais e/ou domésticos. Podendo ser lixiviado ao lençol freático ou absorvido pelas plantas,resultando na redução do crescimento e da produtividade. Esse metal afeta todos os organismos vivos e causa diferentes danos às células. A tolerância a esse metal se deve principalmente ao balanço do estado redox da célula para manter a integridade celular e metabolismo.Assim, foram isoladas bactérias de solo contaminado e não contaminado com Cd, selecionando isolados tolerantes a altas concentrações de diferentes metais (Cd, Ni e Zn), em seguida, foi observado a resposta do sistema antioxidante da bactéria na presença do Cd, a fim de auxiliar no desenvolvimento de metodologias para biorremediar solos contaminados. Foi quantificado MDA e peróxido de hidrogênio e a atividade de diferentes enzimas do sistema antioxidante (SOD, CAT, GR e GST) de duas estirpes do gênero Burkholderia tolerantes a todos os metais testados, uma isolada do solo contaminado com altas concentrações de Cd (estirpe SCMS54) e a outra do solo sem Cd (estirpe SNMS32) em dois tempos de exposição (5 e 12 h). Na estirpe SCMS54, as medidas de estresse (peroxidação lipídica e peróxido de hidrogênio) e a atividade das enzimas antioxidantes (SOD, CAT, GR e GST) da maioria dos tratamento com cádmio aumentaram, esta estirpe também expressa mais isoformas de SOD, CAT e GR, além de acumular 50% mais Cd. Esses resultados mostram que a estirpe SCMS54 (isolada do solo contaminado com Cd) apresenta uma maior diversidade metabólica e plasticidade. Foram analisadas também a resposta dessas duas estirpes ao Ni, observando uma resposta semelhante ao Cd, exceto na expressão da enzima GST, que no estirpe SCMS54 foi induzida na presença do Ni, indicando que essa enzima pode ser essencial na tolerância ao Ni. Portanto, a estirpe isoladado solo contaminado com Cd (SCMS54) foi selecionada para prosseguir os estudos e avaliar os benefícios da interação entre microrganismos tolerantes-plantas de tomate na fitorremediação. Essa técnica é usada remover para metais pesados do solo com um menor impacto e baixos custos. Os microrganismos do solo podem solubilizar e mobilizar metais do solo, atuando como biorremediador. Além da alta tolerância ao Cd, a estirpe SCMS54 produz ácido indol acético (AIA), solubiliza fosfato inorgânico e produz sideroforo, mostrando seu potencial na interação benéfica planta-microorganismo. Quando interagindo com as plantas de tomate expostas ao Cd, essa bactéria diminui a concentração de peróxido da planta e a clorose ocasionado pelo Cd,e reduz a absorção de Cd pela raiz resultando em um aumento da tolerância da planta ao metal pesado altamente tóxico. Assim, a inoculação de plantas de tomate com Burkholderia sp. SCMS54 promove crescimento da planta na presença de Cd, desencadeando um mecanismo que diminui a concentração de Cd nas raízes devido a essa interação benéfica bactéria-raiz da planta.
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Resposta do milho e do tomateiro à inoculação com bactérias diazotróficas isoladas da superfície de folhas / Response of maize and tomato to the inoculation with diazotrophic bacteria isolated from leaf surfaceAndre Alves de Souza 30 August 2017 (has links)
O desenvolvimento de tecnologias alternativas que visem aumentar a disponibilidade e eficiência do uso dos recursos minerais para as plantas aparecem como importante medida para se alcançar um crescimento sustentável da agricultura. Neste contexto, o suprimento de nitrogênio por meios biológicos, utilizando micro-organismos diazotróficos, dos quais os mais estudados são os rizóbios associados simbioticamente às leguminosas, é uma importante alternativa. Porém o sucesso atingido com essa simbiose em leguminosas ainda não foi alcançado em outras famílias de plantas, principalmente por limitações associadas à otimização das combinações planta-bactéria. Em gramíneas, diazotróficos endofíticos têm sido usados como inoculante, com resultados controversos. No entanto, é possível que micro-organismos diazotróficos epifíticos possam apresentar maior vantagem adaptativa devido à menor especificidade em sua relação com a planta, e serem mais eficientes em plantas não-leguminosas. O presente trabalho teve por objetivo avaliar o efeito de um inoculante produzido a partir de bactérias diazotróficas isoladas da filosfera no crescimento de milho e tomateiro. Ensaios foram realizados em condições de casa-de-vegetação, utilizando-se três diferentes isolados de bactérias diazotróficas encapsuladas em esferas de alginato sob diferentes níveis de adubação nitrogenada. A eficiência dos isolados em fixar nitrogênio foi avaliada através da atividade da nitrogenase. As populações de diazotróficos na rizosfera foram determinadas através da técnica do número mais provável (NMP) ao final do ensaio. Além disso, parâmetros como biomassa da parte aérea e das raízes foram analisados. Os dados foram submetidos à análise de variância, sendo as médias das variáveis comparadas pelo teste Duncan (p<0,05), utilizando-se o programa estatístico R. Plantas de milho, aos 60 dias após a inoculação com o isolado J8L, apresentaram matéria seca da raiz e atividade da nitrogenase 26 e 50 % superior ao controle não-inoculado, respectivamente. Plantas de tomateiro, 30 dias após a inoculação com o isolado E5L, apresentaram matéria seca da parte aérea e matéria seca da raiz 39 e 31 % superior ao controle não-inoculado, respectivamente. Parte dos efeitos observados no milho e tomateiro podem ser explicados pela atividade de fixação biológica de nitrogênio e produção de fitormônios, como auxina. A atividade da nitrogenase na rizosfera do milho, 60 dias após a inoculação foi maior no solo sem adição de N. O NMP não apresentou diferenças significativas e nos ensaios com milho e tomateiro, entre plantas inoculadas e não inoculadas, mostrando uma provável influência da comunidade diazotrófica nativa sobre a população final de diazotróficas analisada. De uma maneira geral, os isolados J8L e E5L proporcionaram ganhos significativos para as culturas do milho e tomateiro, mostrando potencial para uso como biofertilizantes. / The development of alternative technologies to increasing availability and use efficiency of mineral resources for plants is an important step for sustainable agricultural production. In this context, the supply of nitrogen by biological means, using diazotrophic microorganisms, of which the most studied are rhizobia symbiotically associated with legumes, is an important alternative. However, the efficiency of rhizobium-legume symbioses has not yet been achieved in other plant families, mainly due to limitations associated with the optimization of plant-bacterial combinations. In grasses, endophytic diazotrophs have been used as inoculant, with controversial results. However, it is possible that epiphytic diazotrophic microorganisms may have adaptive advantages over endophytes, due to the lower specificity in their associations with plants, and be more efficient in non-leguminous plants. The objective of the present work was to evaluate the effect of an inoculant produced with diazotrophic bacteria isolated from the phyllosphere on the growth of maize and tomato. Assays were carried out under greenhouse conditions using three isolates of diazotrophic bacteria encapsulated in alginate beads under different levels of nitrogen fertilization. The efficiency of the isolates in fixing nitrogen was evaluated determining nitrogenase activities. The populations of diazotrophic bacteria in the rhizosphere were determined by the most probable number (MPN) technique at the end of the experiment. In addition, parameters such as shoot and root biomass were analyzed. The data were submitted to the analysis of variance, and means were compared by the Duncan test (p<0.05), using the statistical program R. Maize plants at 60 days after inoculation with the J8L isolate presented root dry weight and nitrogenase activity in the rhizosphere 26 and 50% higher than the uninoculated control, respectively. Tomato plants, 30 days after inoculation with the E5L isolate, presented shoot and root dry weight 39 and 31% higher than the uninoculated control, respectively. Part of the effects observed in maize and tomato can be explained by the nitrogen fixation activity and auxin production. Nitrogenase activity in the maize rhizosphere, 60 days after inoculation was higher in the soil without N supply. The population of diazothrophic bacteria in the rhizosphere of inoculated and non-inoculated maize and tomato plants were not significantly different, showing a probable influence of the native diazotrophic community on the total diazotrophic population. In general, isolates J8L and E5L promoted growth of maize and tomato, showing potential for use as biofertilizers.
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Resposta do milho e do tomateiro à inoculação com bactérias diazotróficas isoladas da superfície de folhas / Response of maize and tomato to the inoculation with diazotrophic bacteria isolated from leaf surfaceSouza, Andre Alves de 30 August 2017 (has links)
O desenvolvimento de tecnologias alternativas que visem aumentar a disponibilidade e eficiência do uso dos recursos minerais para as plantas aparecem como importante medida para se alcançar um crescimento sustentável da agricultura. Neste contexto, o suprimento de nitrogênio por meios biológicos, utilizando micro-organismos diazotróficos, dos quais os mais estudados são os rizóbios associados simbioticamente às leguminosas, é uma importante alternativa. Porém o sucesso atingido com essa simbiose em leguminosas ainda não foi alcançado em outras famílias de plantas, principalmente por limitações associadas à otimização das combinações planta-bactéria. Em gramíneas, diazotróficos endofíticos têm sido usados como inoculante, com resultados controversos. No entanto, é possível que micro-organismos diazotróficos epifíticos possam apresentar maior vantagem adaptativa devido à menor especificidade em sua relação com a planta, e serem mais eficientes em plantas não-leguminosas. O presente trabalho teve por objetivo avaliar o efeito de um inoculante produzido a partir de bactérias diazotróficas isoladas da filosfera no crescimento de milho e tomateiro. Ensaios foram realizados em condições de casa-de-vegetação, utilizando-se três diferentes isolados de bactérias diazotróficas encapsuladas em esferas de alginato sob diferentes níveis de adubação nitrogenada. A eficiência dos isolados em fixar nitrogênio foi avaliada através da atividade da nitrogenase. As populações de diazotróficos na rizosfera foram determinadas através da técnica do número mais provável (NMP) ao final do ensaio. Além disso, parâmetros como biomassa da parte aérea e das raízes foram analisados. Os dados foram submetidos à análise de variância, sendo as médias das variáveis comparadas pelo teste Duncan (p<0,05), utilizando-se o programa estatístico R. Plantas de milho, aos 60 dias após a inoculação com o isolado J8L, apresentaram matéria seca da raiz e atividade da nitrogenase 26 e 50 % superior ao controle não-inoculado, respectivamente. Plantas de tomateiro, 30 dias após a inoculação com o isolado E5L, apresentaram matéria seca da parte aérea e matéria seca da raiz 39 e 31 % superior ao controle não-inoculado, respectivamente. Parte dos efeitos observados no milho e tomateiro podem ser explicados pela atividade de fixação biológica de nitrogênio e produção de fitormônios, como auxina. A atividade da nitrogenase na rizosfera do milho, 60 dias após a inoculação foi maior no solo sem adição de N. O NMP não apresentou diferenças significativas e nos ensaios com milho e tomateiro, entre plantas inoculadas e não inoculadas, mostrando uma provável influência da comunidade diazotrófica nativa sobre a população final de diazotróficas analisada. De uma maneira geral, os isolados J8L e E5L proporcionaram ganhos significativos para as culturas do milho e tomateiro, mostrando potencial para uso como biofertilizantes. / The development of alternative technologies to increasing availability and use efficiency of mineral resources for plants is an important step for sustainable agricultural production. In this context, the supply of nitrogen by biological means, using diazotrophic microorganisms, of which the most studied are rhizobia symbiotically associated with legumes, is an important alternative. However, the efficiency of rhizobium-legume symbioses has not yet been achieved in other plant families, mainly due to limitations associated with the optimization of plant-bacterial combinations. In grasses, endophytic diazotrophs have been used as inoculant, with controversial results. However, it is possible that epiphytic diazotrophic microorganisms may have adaptive advantages over endophytes, due to the lower specificity in their associations with plants, and be more efficient in non-leguminous plants. The objective of the present work was to evaluate the effect of an inoculant produced with diazotrophic bacteria isolated from the phyllosphere on the growth of maize and tomato. Assays were carried out under greenhouse conditions using three isolates of diazotrophic bacteria encapsulated in alginate beads under different levels of nitrogen fertilization. The efficiency of the isolates in fixing nitrogen was evaluated determining nitrogenase activities. The populations of diazotrophic bacteria in the rhizosphere were determined by the most probable number (MPN) technique at the end of the experiment. In addition, parameters such as shoot and root biomass were analyzed. The data were submitted to the analysis of variance, and means were compared by the Duncan test (p<0.05), using the statistical program R. Maize plants at 60 days after inoculation with the J8L isolate presented root dry weight and nitrogenase activity in the rhizosphere 26 and 50% higher than the uninoculated control, respectively. Tomato plants, 30 days after inoculation with the E5L isolate, presented shoot and root dry weight 39 and 31% higher than the uninoculated control, respectively. Part of the effects observed in maize and tomato can be explained by the nitrogen fixation activity and auxin production. Nitrogenase activity in the maize rhizosphere, 60 days after inoculation was higher in the soil without N supply. The population of diazothrophic bacteria in the rhizosphere of inoculated and non-inoculated maize and tomato plants were not significantly different, showing a probable influence of the native diazotrophic community on the total diazotrophic population. In general, isolates J8L and E5L promoted growth of maize and tomato, showing potential for use as biofertilizers.
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Effects of a bacterial ACC deaminase on plant growth-promotionCzarny, Jennifer Claire January 2008 (has links)
Plants often live in association with growth-promoting bacteria, which provide them with several benefits. One such benefit is the lowering of plant ethylene levels through the action of the bacterial enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase that cleaves the immediate biosynthetic precursor of ethylene, ACC. The plant hormone ethylene is responsible for many aspects of plant growth and development but under stressful conditions ethylene exacerbates stress symptoms. The ACC deaminase-containing bacterium Pseudomonas putida UW4, isolated from the rhizosphere of reeds, is a potent plant growth-promoting strain and as such was used, along with an ACC deaminase minus mutant of this strain, to study the role of ACC deaminase in plant growth-promotion. Also, transgenic plants expressing a bacterial ACC deaminase gene were used to study the role of this enzyme in plant growth and stress tolerance in the presence and absence of nickel. Transcriptional changes occurring within plant tissues were investigated with the use of an Arabidopsis oligonucleotide microarray.
The results showed that transcription of genes involved in hormone regulation, secondary metabolism and the stress response changed in all treatments. In particular, the presence of ACC deaminase caused genes for auxin response factors to be up-regulated in plant tissues suggesting a de-repression of auxin signaling in the absence of high levels of ethylene. Also, transgenic plants had longer roots and grew faster than the non-transformed plants and genes involved in the stress response and secondary metabolism were up-regulated. Plants inoculated with bacteria had lower levels of secondary metabolism gene expression and slightly higher stress response gene expression than uninoculated plants. Yet, inoculation with the ACC deaminase-expressing bacterium caused less up-regulation of plant genes involved in stress and defense responses and the down-regulation of genes involved in nitrogen metabolism in comparison to plants inoculated with the ACC deaminase minus mutant.
Nickel stress caused the down-regulation of genes involved in photosynthesis and carbon fixation and the up-regulation of genes involved in stress responses, and amino acid and lipid breakdown suggesting energy starvation. When transgenic plants expressing ACC deaminase in the roots were exposed to nickel stress, plant stress symptoms were significantly lower and biomass was significantly higher suggesting that lowering the level of ethylene relieved many of the stress symptoms. In fact, genes involved in photosynthesis, secondary metabolism and nitrate assimilation were up-regulated in transgenic plants compared with non-transformed plants in the presence of nickel, suggesting that ACC deaminase is effective at reducing the severe effects of this metal stress.
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Effects of a bacterial ACC deaminase on plant growth-promotionCzarny, Jennifer Claire January 2008 (has links)
Plants often live in association with growth-promoting bacteria, which provide them with several benefits. One such benefit is the lowering of plant ethylene levels through the action of the bacterial enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase that cleaves the immediate biosynthetic precursor of ethylene, ACC. The plant hormone ethylene is responsible for many aspects of plant growth and development but under stressful conditions ethylene exacerbates stress symptoms. The ACC deaminase-containing bacterium Pseudomonas putida UW4, isolated from the rhizosphere of reeds, is a potent plant growth-promoting strain and as such was used, along with an ACC deaminase minus mutant of this strain, to study the role of ACC deaminase in plant growth-promotion. Also, transgenic plants expressing a bacterial ACC deaminase gene were used to study the role of this enzyme in plant growth and stress tolerance in the presence and absence of nickel. Transcriptional changes occurring within plant tissues were investigated with the use of an Arabidopsis oligonucleotide microarray.
The results showed that transcription of genes involved in hormone regulation, secondary metabolism and the stress response changed in all treatments. In particular, the presence of ACC deaminase caused genes for auxin response factors to be up-regulated in plant tissues suggesting a de-repression of auxin signaling in the absence of high levels of ethylene. Also, transgenic plants had longer roots and grew faster than the non-transformed plants and genes involved in the stress response and secondary metabolism were up-regulated. Plants inoculated with bacteria had lower levels of secondary metabolism gene expression and slightly higher stress response gene expression than uninoculated plants. Yet, inoculation with the ACC deaminase-expressing bacterium caused less up-regulation of plant genes involved in stress and defense responses and the down-regulation of genes involved in nitrogen metabolism in comparison to plants inoculated with the ACC deaminase minus mutant.
Nickel stress caused the down-regulation of genes involved in photosynthesis and carbon fixation and the up-regulation of genes involved in stress responses, and amino acid and lipid breakdown suggesting energy starvation. When transgenic plants expressing ACC deaminase in the roots were exposed to nickel stress, plant stress symptoms were significantly lower and biomass was significantly higher suggesting that lowering the level of ethylene relieved many of the stress symptoms. In fact, genes involved in photosynthesis, secondary metabolism and nitrate assimilation were up-regulated in transgenic plants compared with non-transformed plants in the presence of nickel, suggesting that ACC deaminase is effective at reducing the severe effects of this metal stress.
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The relationship between Sarracenia oreophila and an endophytic BurkholderiaKuntz, Veronica L. 17 May 2011 (has links)
Plant growth-promoting bacteria (PGPB) have been studied in many agriculturally interesting plants, but never in pitcher plants.
Sarracenia oreophila (the green pitcher plant) is an endangered species in Georgia, Alabama, and North Carolina (Rice 2010). With the help of Dr. Jim Spain's lab, a previous student in Dr. Gerald Pullman's lab discovered evidence that nitrogen-fixing bacteria (Burkholderia spp.) live within these pitcher plants. This study aims to determine whether these nitrogen-fixing bacteria confer a benefit to their host plants by providing fixed nitrogen.
To do this, pitcher plants were inoculated with the Burkholderia and grown on a control medium, a medium without sugar (as the sugar causes the bacteria to grow until they hinder the plants), various media that are missing nitrogen-containing compounds usually provided in growth media, and a medium completely lacking nitrogen. These plants were compared to control plants on the same media that had not been inoculated with Burkholderia. The plants' biomass and root growth were measured.
The data suggest that Burkholderia may stimulate plant biomass growth when sufficient nitrogen is present and there may be a nitrogen-threshold that needs to be met in order to sustain the Burkholderia-Sarracenia symbiosis. Also, the Burkholderia has a negative effect on roots grown in high-nitrogen media, possibly due to competition for nutrients.
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Burkholderia sp. cadmium tolerance mechanism and its influence in phytoremediation / Mecanismos de tolerância ao cádmio em Burkholderia sp. e sua aplicação na fitorremediaçãoManuella Nóbrega Dourado Ribeiro 22 November 2013 (has links)
Soils have been contaminated with cadmium (Cd) by the use of fertilizers, calcareous, pesticides and industrial and/or domestic effluents. It can be leached to groundwater, as well as be taken up by plants potentially leading to reduce growth and yield. It causes different damages to the cell, generating oxidative stress which is responsible for its toxicity, affecting all living organism. A balance in the redox state of the cell to maintain cellular integrity and metabolism is essential for organism tolerance. Thus, the antioxidant response of bacteria exposed to Cd was studied to understand the tolerance mechanism, and be able to develop a methodology to bioremediate contaminated soils. MDA and hydrogen peroxide contents and different enzymes activity of antioxidant system (SOD, CAT, GR and GST) of two strains from Burkholderia genus, one from a soil contaminated with Cd in high concentrations (strain SCMS54) and the other from soil without Cd (strain SNMS32) in two exposure time (5 and 12 h), were analyzed. Stress measurement (MDA and hydrogen peroxide content) and antioxidant enzyme activities (SOD, CAT, GR and GST) increased in almost all treatments in the presence of Cd. These results also indicate that strain SCMS54 (isolated from Cd contaminated soil) presents a higher metabolic diversity and plasticity due the expression of more isoforms of the enzymes SOD, CAT and GR. The strain also accumulates 50% more Cd. We also analyzed the response to Ni of these two strain, observing a similar response to Cd, except for GST enzyme expression, which in strain SCMS54 this enzyme was induced in the presence of Ni, indicating that this enzyme can be essential on Ni tolerance. After that, the strain isolated from Cd contaminated soil (SCMS54) was selected to proceed the studies to evaluate the benefits of tolerant microorganism-tomato plant interaction. The use of plants to remove heavy metals from contaminated soilhas less impact and a lower cost. Soil microorganisms can be able to solubilize or mobilize soil metals acting also as bioremediator. Besides the high tolerance to Cd, the strain SCMS54 can produce indole-acetic acid (IAA), solubilize inorganic phosphate and produce siderophore, revealing its potential in plantmicroorganism mutual and beneficial interaction. When interacting with tomato plants exposed to Cd, this bacterium led to decrease in plant peroxide concentration and chlorosis levels, promoted relative plant growth and reduced the root absorption of Cd resulting in an increase in plant tolerance to this highly toxic heavy metal. Indicating that inoculation of tomato plants with Burkholderia sp. SCMS54 promotes better growth when cultivated in the presence of Cd by a mechanism that appears to decrease Cd concentration in roots as a result of a bacterial-plant root beneficial interaction. / O cádmio (Cd) tem contaminado solos pelo uso de fertilizantes, calcário, agrotóxicos e resíduos industriais e/ou domésticos. Podendo ser lixiviado ao lençol freático ou absorvido pelas plantas,resultando na redução do crescimento e da produtividade. Esse metal afeta todos os organismos vivos e causa diferentes danos às células. A tolerância a esse metal se deve principalmente ao balanço do estado redox da célula para manter a integridade celular e metabolismo.Assim, foram isoladas bactérias de solo contaminado e não contaminado com Cd, selecionando isolados tolerantes a altas concentrações de diferentes metais (Cd, Ni e Zn), em seguida, foi observado a resposta do sistema antioxidante da bactéria na presença do Cd, a fim de auxiliar no desenvolvimento de metodologias para biorremediar solos contaminados. Foi quantificado MDA e peróxido de hidrogênio e a atividade de diferentes enzimas do sistema antioxidante (SOD, CAT, GR e GST) de duas estirpes do gênero Burkholderia tolerantes a todos os metais testados, uma isolada do solo contaminado com altas concentrações de Cd (estirpe SCMS54) e a outra do solo sem Cd (estirpe SNMS32) em dois tempos de exposição (5 e 12 h). Na estirpe SCMS54, as medidas de estresse (peroxidação lipídica e peróxido de hidrogênio) e a atividade das enzimas antioxidantes (SOD, CAT, GR e GST) da maioria dos tratamento com cádmio aumentaram, esta estirpe também expressa mais isoformas de SOD, CAT e GR, além de acumular 50% mais Cd. Esses resultados mostram que a estirpe SCMS54 (isolada do solo contaminado com Cd) apresenta uma maior diversidade metabólica e plasticidade. Foram analisadas também a resposta dessas duas estirpes ao Ni, observando uma resposta semelhante ao Cd, exceto na expressão da enzima GST, que no estirpe SCMS54 foi induzida na presença do Ni, indicando que essa enzima pode ser essencial na tolerância ao Ni. Portanto, a estirpe isoladado solo contaminado com Cd (SCMS54) foi selecionada para prosseguir os estudos e avaliar os benefícios da interação entre microrganismos tolerantes-plantas de tomate na fitorremediação. Essa técnica é usada remover para metais pesados do solo com um menor impacto e baixos custos. Os microrganismos do solo podem solubilizar e mobilizar metais do solo, atuando como biorremediador. Além da alta tolerância ao Cd, a estirpe SCMS54 produz ácido indol acético (AIA), solubiliza fosfato inorgânico e produz sideroforo, mostrando seu potencial na interação benéfica planta-microorganismo. Quando interagindo com as plantas de tomate expostas ao Cd, essa bactéria diminui a concentração de peróxido da planta e a clorose ocasionado pelo Cd,e reduz a absorção de Cd pela raiz resultando em um aumento da tolerância da planta ao metal pesado altamente tóxico. Assim, a inoculação de plantas de tomate com Burkholderia sp. SCMS54 promove crescimento da planta na presença de Cd, desencadeando um mecanismo que diminui a concentração de Cd nas raízes devido a essa interação benéfica bactéria-raiz da planta.
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Molecular perception and metabolic rewiring of the host plant by beneficial microbe Enterobacter sp. SA187Alzayed, Waad S. 10 1900 (has links)
Among abiotic stresses, salinity is considered the main limiting stress that negatively affects plant growth and reduces productivity worldwide. To overcome this challenge, a sustainable solution such as plant growth-promoting bacteria (PGPB) can be used to meet the increasing demand for food. The desert microbe Enterobacter sp SA187, an endophytic PGPB, induces salt tolerance in both model plant and crops. The interaction between SA187 and the host plant triggers the sulfur pathway in the bacteria which then provides multiple sulfur-containing compounds to its host plant. However, the molecular sensor of these compounds in the host plant is not known. Here, we show that SA187 activates the plant target of rapamycin (TOR) pathway. The beneficial effect of SA187 was lost in TOR mutants like raptor, and by the application of TOR inhibitor AZD8055. Next, we show that SA187 modulates the one- carbon (1C) metabolism of the host plant consisting of methionine and folate cycles. The beneficial effect of SA187 was compromised by using chemical inhibitors of folate cycle like Methotrexate (MTX) and Sulfadiazine (SDZ). The intermediates of the 1C metabolism like Homocysteine and S-adenosyl methionine (SAM) showed similar beneficial effects as SA187 colonized plants. Finally, we showed that SA187 enhances 1C metabolism activity by increasing methylation index (SAM/SAH ratio) in the plants. Taken together, we could show that host TOR-1C axis is essential for plant salt
tolerance by SA187.
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