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Three Dimensional Simulation of Rayleigh-Bénard Convection for Rapid Microscale Polymerase Chain ReactionMuddu, Radha Malini Gowri 2010 December 1900 (has links)
Rayleigh-Bénard convection has been extensively studied in literature owing to its ubiquitous nature. However, most of the studies have been confined to geometries where the aspect ratio of the cylinder was less than 1. Here we study the motion of fluid in geometries with aspect ratio greater than 1, with particular application to use of such motion to actuate biochemical reactions, such as the polymerase chain reaction. We show that it is possible to accelerate the rate of reaction by using a geometry that promotes chaotic motion versus a geometry that promotes quasi- periodic motion. We also simulate chemical kinetics using the fluid motion as a starting point and we prove that chaotic motion indeed enhances the rate of the reaction. We also provide qualitative and quantitative measures for chaotic motion in a fluid flow, which helps to distinguish between different types of fluid motion. We highlight the transitions between different types of flow that are possible with Rayleigh-Bénard convection. Finally, we compare our simulations against experimental data obtained from particle image velocimetry, laser induced fluorescence and optical microscopic visualization.
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Determination of PTEN mutations in prostate cancer in ChineseTsui, Wai-yan. January 2001 (has links)
Thesis (M. Med. Sc.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 32-36).
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Sequence-specific electrochemical DNA detection and its implementation in integrated PCR-electrochemical microdevices /Lee, Thomas Ming Hung. January 2003 (has links)
Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2003. / Includes bibliographical references (leaves 155-177). Also available in electronic version. Access restricted to campus users.
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Evaluation of a multiplex polymerase chain reaction assay for detection of beta-lactam resistance in streptococcus pneumoniaeWong, Chun-wai, 黃振威 January 2003 (has links)
published_or_final_version / Medical Sciences / Master / Master of Medical Sciences
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The detection and quantification of genetically modified E. coli using PCRCornett, Johanne Hazel January 2000 (has links)
No description available.
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Selector technology : for multiplex DNA analysis /Dahl, Fredrik, January 2005 (has links)
Diss. (sammanfattning) Uppsala : Uppsala universitet, 2005. / Härtill 4 uppsatser.
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Selectivity in palladium- and enzyme-catalyzed reactions : focusing on enhancement of reactivity /Nilsson, Peter, January 2003 (has links)
Diss. (sammanfattning) Uppsala : Univ., 2003. / Härtill 5 uppsatser.
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Rapid detection of Norwalk-like viruses (NLV's) /Wati, Satiya. January 1999 (has links) (PDF)
Thesis (M.Sc.) -- University of Adelaide, Dept. of Microbiology and Immunology, 2000? / Bibliography: leaves 106-128.
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Development of a real-time quantitative polymerase chain reaction method to quantify Lactobacillus buchneri in order to study its growth and effects in silages when added alone or in combination with Pediococcus pentosaceusSchmidt, Renato Jose. January 2008 (has links)
Thesis (Ph. D.)--University of Delaware, 2008. / Principal faculty advisor: Limin Kung, Jr., Dept. of Animal & Food Sciences. Includes bibliographical references.
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Gene expression of components of the calpain system m-calpain, [mu]-calpain and calpastatin in male and female broiler skeletal muscle /Janardhanan, Anitha C. January 1999 (has links)
Thesis (M.S.)--West Virginia University, 1999. / Title from document title page. Document formatted into pages; contains vii, 93 p. : ill. (some col.) Includes abstract. Includes bibliographical references (p. 72-80).
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