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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Evaluation and expression of indolicidins and polyphemusin variants in plants for broad-spectrum disease resistance

Bhargava, Apurva January 2005 (has links)
The cationic antimicrobial peptides, indolicidins and polyphemusins, have shown activity against animal pathogenic viruses. The aim of this work was to evaluate the efficacy of these peptides for engineering plants with a broad-spectrum disease resistance, including viral diseases. TTC, Evan's blue and phytotoxicity assays showed low cytotoxicity of indolicidin and polyphemusin variants (10R, 11R and PV5) on tobacco leaf discs, protoplasts and plantlets. In vitro assays using these variants showed a broad-spectrum activity against plant pathogenic bacteria, fungi and viruses. An assessment of in planta activity was performed by expressing these cationic peptides in transgenic tobacco (Nicotiana tabacum var Xanthi). The disease assays using detached leaves from transgenic tobacco plants showed a broad-spectrum disease resistance against bacteria, fungi and 'TMV. Further work is needed on optimization of expression of these peptides and their combinations for successful application of this approach under greenhouse and field conditions.

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