The study aims to present the notion of rhythmanalysis in relation to the city environment and aims to apply the theory to field research. The theoretical part focuses on the semiotic conception of the rhythm, on the position of rhythmanalysis in the work of its father, Henri Lefebvre and it further tackles the topic and its applications in Czech and also international context. Following this approach, it clarifies a wide range of related conceptions and phenomenons. It finds common points of reference in philosophy, semiotics, critical theory, urbanism, social geography, art and other fields, which is determined by its interdisciplinary nature. The practical part relates the previously mentioned theoretical foundations to applied, empirical research. It observes and reveals the rhythms of Prague area named Vltavská, it analyzes and interprets them and shows the rhythmanalysis as a legitimate analytical instrument with a potential value for urbanism.