I used mitochondrial COI sequence data from forty one individuals to investigate phylogenetic relationships among populations of two morphologically similar species of the pseudoscorpion genus Apochthonius, A. minimus and A. occidentalis, in western Washington, Oregon, and northern California. My goal was to assess whether genetic structure in the two species was congruent with geography. Many plant and animal species in the Pacific Northwestern United States have shown patterns of genetic differentiation that follow both north-south and east-west trends, indicating that geologic and climatic events in the past separated populations to the extent that they became genetically differentiated. A distinct geographic pattern emerged within A. occidentalis, with at least one northern and two southern populations. A clade containing all A. minimus sequences was recovered. However, this clade falls within the larger clade of A. occidentalis, rendering A. occidentalis paraphyletic. Furthermore, the A. minimus sequences showed north-south geographic structuring within the clade. Population genetic analyses were performed based on geographic location within the Pacific Northwest. I found high genetic differentiation coupled with low gene flow between most populations, with the exception of the Portland and North Coast Range populations. These data suggest the presence of more than two species of Apochthonius in the Pacific Northwest.

Pieris oleracea, formerly Pieries napi, was once a widespread pierid butterfly in New England until the introduction of a biological control agent, Cotesia glomerata. It has been suggested that C. glomerata is responsible for the range reduction of P. oleracea. There are been several introductions of a second more specialized biological control agent, Cotesia rubecula, to the United States since the 1960’s. My first goal was to determine the current distribution and status of P. rapae parasitoids and the effectiveness of C. rubecula as a biological control agent since its release. The findings of a survey I conducted of the parasitoid community of P. rapae indicate that C. rubecula now occurs as far west as North Dakota and has become the dominant parasitoid of P. rapae in the northeastern and north central United States and adjacent parts of southeastern Canada, where it has displaced C. glomerata, the previously dominant parasitoid. Survival of artificially established cohorts of P. rapae larvae was assessed in a collard patch on an organic vegetable farm in western Massachusetts. There was a significant drop in larval survival between the 4th and 5th instar due to parasitism by C. rubecula. This was change from survival curves of P. rapae from a 1985-1986 study, in which there was a significant drop in survival between the 5th instar and pupal stage due to C. glomerata. The final goal of my thesis work is to try to understand why P. oleracea was able to survive at the focal study site in Lenox, MA despite parasitoid pressure and range reduction elsewhere in New England. In olfactometer tests, there was no difference in attractiveness of naïve C. glomerata females to volatiles of either Cardamine pratensis (cuckooflower) foliage, the host plant of P. oleracea or Brassica olercea (collard) foliage (P = 0.51). In order to determine if overtopping by other vegetation may provide an enemy free space for P. oleracea by affecting detection by C. glomerata, cage experiments were conducted. Overtopping vegetation had a significant effect on parasitism by C. glomerata (F = 12.8, df = 3, PP. oleracea has been able to thrive at the Lenox, MA site.

Translocations are an important aspect of the management of natural populations in an increasingly fragmented landscape. Maintaining connectivity and gene flow is beneficial for both contemporary fitness and adaptive potential in the face of environmental change. Genetic rescue (GR) can alleviate inbreeding depression, genetic load, and increase adaptive potential of populations. Here, I have translocated 10 (5 of each sex) brook trout (Salvelinus fontinalis) to four geographically proximate and environmentally similar fragmented stream-dwelling populations of brook trout in Virginia to test for genetic rescue. The translocated brook trout contributed to more families than would be expected under neutral introgression, and hybridization resulted consistently in larger full-sibling family sizes. In the cohort immediately following translocation I observed relatively high (>20%) introgression in 3 of the 4 recipient sites, and in one recipient population 57.7 % of the offspring had at least one migrant parent. During the post-translocation period favorable regional climatic conditions resulted in large cohorts across recipient sites and controls, however the percent increase in juvenile abundance scales to initial genetic diversity and patch size. I observe strong evidence of hybrid vigor through consistently larger body sizes of hybrid offspring. At this point I cannot rule out potential negative effects of translocations such as outbreeding depression with out sampling more cohorts following genetic recombination. However, I provide an empirical and replicated foundation to begin assessing the efficacy of GR-motivated translocations for headwater fish conservation, and make a substantial contribution to the growing body of GR-literature.

Long-term population dynamics across heterogeneous environments can be a major factor in determining species’ ability to expand their ranges and persist in novel environments. Whether and how the relative performance of populations in different microsites over time impacts invasion into new microsites is poorly understood. Though largely restricted to disturbed semi-shaded microhabitats in its home range, the invasive herb Alliaria petiolata (garlic mustard) successfully invades intact forest understories – a novel microhabitat – in its introduced range, where it is known to impact above and below ground community composition. To test the hypothesis that source-sink metapopulation dynamics may be promoting A. petiolata’s incursion into the forest understory, I utilized two multi-season field surveys – approximately a decade apart – to evaluate trait variation, biomass allocation, and long-term population demographics of A. petiolata growing at the forest edge, within the intact forest understory, and in the intermediate transition zone between the two. My results show that adult plants in the edge were taller and branchier, produced more fruits, and had higher total and reproductive biomass than plants in the intermediate and forest microhabitats. Over time, seedling density remained highest in the edge microhabitat compared to the forest and intermediate microhabitats, which had similar densities. Reproductive adult densities were similar among all microhabitats at the beginning of the study, but a decade later, all microhabitats exhibited a decline in the number of adult plants they supported. Populations in the intermediate microhabitat displayed the steepest decline in reproductive adults between sampling periods but still supported more adult plants than the forest microhabitat. Populations in all microhabitats were predicted to grow (λ>1) at the onset of the study. A decade later, declines in population size were only predicted in the forest understory (λ1). Since edge and intermediate patches had higher densities of adult plants which produced the most fruit and had larger reproductive biomass, it appears that the edge populations, and possibly the intermediate populations, have sustained the low-density forest populations through source-sink dynamics at my study sites.

Atlantic horseshoe crabs (Limulus polyphemus) have endured decades of intense harvest pressure. Genetics studies have shown evidence of distinct sub-groups spanning the coast, although few fine-scale studies have been done to delineate these groups on a local level. Massachusetts lies directly between two of these sub-groups. With documented differences in prosomal widths of horseshoe crabs from either side of Cape Cod, it is possible that Cape Cod is a barrier to gene flow and that there are two distinct genetic groups within Massachusetts. Regulations currently consider all horseshoe crabs to be of one stock. I examined 6 microsatellite loci from 193 horseshoe crabs collected from 7 locations across Massachusetts between 5 May and 24 June 2010. I also analyzed the prosomal widths of 324 horseshoe crabs from 8 locations across Massachusetts. Data analysis revealed low divergence with a G′ST of 0.005 (95% CI −0.004–0.013) and a G″ST of 0.015 (95% CI −0.014–0.045). Wellfleet Bay showed evidence of divergence from all other sites except Buzzards Bay. Isolation by distance is apparent via the Atlantic Ocean. Phenotypic variation in the prosomal widths of horseshoe crabs shows greater divergence among sites than neutral markers and indicates the presence of additive genetic effects. Low divergence and high heterozygosity indicate that although documented population declines have occurred, effective population size (Ne) is still large enough to maintain allele frequencies. With isolation by distance, divergence is likely to increase over time if populations remain low. Phenotypic divergence shows the possibility of local adaptation and that the implementation of management units (MUs) to the north and south of Cape Cod would be recommended as a conservative measure.

Three separate studies of the effect of plant pathogen on population biology of its host are presented in the thesis. Two are using field data about a widespread system of plant Falcaria vulgaris and its systemic rust fungus Puccinia sii-falcariae. The first study shows, that the disease prevalences in 40 populations of the plant were correlated with the moisture, the soil reaction and the cover of the herb layer at the localities. This was probably a result of the interaction of the life history of the plant and different effect the disease has at various localities. Similar pattern was found in the second study that aimed to determine long-term effect of the disease at the population level at four chosen localities over 4-5 years. Population growth rates were only rarely predicted to be higher for the healthy part of the population when compared with the whole population. Other analyses have however found big differences among years and localities. The locality type (slope vs. field populations) was important factor influencing population stage composition and importance of life cycle transitions for the growth of the population. Finally, the last study explores the possibility that systemic infection in clonal plants might be able to select against clonality. The result of the modelling showed that more...

When new populations are first identified in a region there are multiple potential sources: introduction of a non-native species, extra-range expansion of a nearby population, or demographic growth of a previously unnoticed species. Red foxes were absent or rare in the mid-eastern portion United States until the late 1800s. Their origins potentially include natural population increase/expansion, translocations from Europe, and, eventually, 20th century fur farming. In this study I attempt to identify the relative impact of native expansion versus human mediated introductions of both colonial era European foxes and early 20th century fur-farm foxes on the establishment of red foxes in the mid-Atlantic region of the United States. I subsequently address the potential impacts of hybridization and nuclear introgression between previously separate sister taxa. Through analysis of mitochondrial DNA, I identified indigenous haplotypes, two European haplotypes, and fur-farm haplotypes; another set of haplotypes were potentially indigenous or native. In addition, I found European Y-chromosome haplotypes. Most European and fur-farm haplotypes were found near the densely human-populated coastal plain and Hudson River lowlands; most red foxes of the Appalachians and Piedmont had native eastern haplotypes. However, nuclear data does not support this division showing low genetic structure despite the broad geographic scale of our study area, attributable both to range expansion and admixture. Admixture has not had the same impact on the nuclear genome as it has in mitochondrial haplotypes leading to mito-nuclear discordance across the region. I also found evidence for differential patterns of expansion related to habitat. Specifically, the Appalachian Mountains acted as a corridor for gene flow from the northern native source into the southern Mid-Atlantic region

To assess the status of a rare plant species, we must first understand the factors that affect the size of populations and their numbers. In this thesis, I study processes affecting plant species prosperity on local scale (paper 1), factors influencing species distribution in landscape and ability of species to colonize new habitats (paper 2) and species traits responsible for gene flow between established populations (paper 3). Since I work with rare plants, I also aim to turn theoretical knowledge into practical recommendations for nature conservation (paper 4) to help effectively preserve rare and endangered species. In the first 3 papers, I work with two rare fern species restricted to serpentine rocks, Asplenium adulterinum and A. cuneifolium, in a study system covering 10 × 10 km. I found that both species are long living (several decades) and in the study region, populations are in a good state and slowly growing. Even very small populations (10 individuals) have quite high chance to survive. In both fern species, I found dispersal limitation, which might be surprising regarding huge production of small spores in ferns (paper 2). The species differ in ploidy and thus, also mating system. A. adulterinum is tetraploid and its main breeding system is intragametophytic selfing. A. cuneifolium is...

In einem 45 ha großen Untersuchungsgebiet in der Innenstadt von Berlin wurden von Februar 1996 bis Juni 1999 alle dort vorkommenden verwilderten Katzen erfaßt. Wir untersuchten Alter- und Geschlechtsverteilung, Populationsdichte und -stabilität, Reproduktions- und Mortalitätsraten, Gesundheitsstatus, Territorialverhalten, Ver- wandtschaftsbeziehungen und den Einfluß der Kastration auf das individuelle Verhal- ten der Katzen und die Entwicklung der Population. Insgesamt wurden 75 Tiere in- nerhalb von 42 Monaten beobachtet. Im Untersuchungsgebiet waren gleichzeitig je- weils 25-32 Tiere ansässig, außerdem wurden durchschnittlich 10 Durchzügler pro Jahr registriert. Die ansässigen Katzen lebten in 2 genetisch differenzierten Teilpopu- lationen. Unkastrierte Kater hatten größere Streifgebiete (20-66 ha) als kastrierte Ka- ter, kastrierte und unkastrierte Katzen sowie Jungtiere (0,5-7,5 ha). Die Mortalität der ansässigen Katzen betrug 25 % pro Jahr. Vakante Streifgebiete wurden durch eige- ne Nachkommen aufgefüllt. Die Anzahl unkastrierter adulter Kater im Untersu- chungsgebiet blieb über die gesamte Zeit konstant, Kastration und Tod von Deckka- tern wurde durch Einwanderung fremder Kater kompensiert. Nach Kastration aller weiblicher Katzen einer Teilpopulation sank die Populationsdichte trotz signifikant höherer Zuwanderung fremder Tiere. / In a 45 ha study area of Berlin city all cats were studied between February 1996 and June 1999. We investigated age and sex composition, population density and stabil- ity, rates of reproduction and mortality, health status, territorial behaviour, kinship re- lation, and the influence of neutering on individual behaviour and population devel- opment. Altogether we observed 75 cats within 42 months. 25 to 35 cats lived simul- taneously in the study area, additionally we found 10 transient animals per year on average. The resident cats lived in two genetically distinct subpopulations. Uncas- trated adult males had larger home ranges (20-66 ha) than castrated males, cas- trated and uncastrated females and subadult cats (0,5-7,5 ha). Mortality rate of resi- dent cats was 25 % per year. Vacancies were filled by own kittens. The number of uncastrated adult tomcats was constant throughout the study period, castration and death of stud males were compensated by immigration of unknown tomcats. After the castration of all females in one subpopulation population density declined, even though immigration of unknown cats increased significantly.

For over a decade, researchers at Harbor Branch Oceanographic Institute (HBOI) have conducted surveys of the bottlenose dolphin (Tursiops truncatus) population of Indian River Lagoon (IRL) in Florida. I have constructed a 4-stage population model using the statistical program R. The model is used to conduct a viability analysis by analyzing the relationship between birth, calf and adult survival rates. The power analysis compares survey frequency to expected confidence intervals in estimating abundance. The sensitivity analysis shows that the population is most sensitive to changes in adult survival, followed by birth rate and calf survival. The model shows a strong chance of viability over a 50 year time span. The population is vulnerable to long periods of decline if birth, calf or adult survival rates fall below certain thresholds. Overall, the model simulates the future impacts of demographic change, providing a tool for conservation efforts. / Includes bibliography. / Thesis (M.S.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection