This diploma thesis, entitled "Monitoring System for Determination of Motility and Position of Laboratory Animals After Anesthesia", focuses on the design and implementation of contactless detection of the position of a rat or mouse in an enclosure with a transparent cover. The aim of the semester work is to find suitable methods of realization of contactless detection of rat or mouse position and to automatically determine and display average speed or other movement characteristics. The assignment arose from the needs of animal monitoring after curative intervention and also as a necessary utility for future "shading" animal movement (automatic targeting of the scar on the animal's back). The rat, which is located inside our enclosure, is either moving as standard or is dazed after anesthesia. In this work I deal first with search of automatic monitoring systems for detection of animals in the enclosure. Then in the practical part are tested three types of cameras for visual detection of rat position and a script for automatic detection and analysis of rat movement is designed. The system works like a camera eye which in real time is able to find the area of a black box in its field of view and then limit the detection area to the size of this box and then automatically detects the center of gravity and counts. and evaluates the obtained speed with an average calculated with a test of 10 mice - voices on the screen the mouse status in the previous ten seconds. for no stressed animal The rat that is located inside our enclosure is either standard or movable after anesthesia. In this work I first deal with searches of automatic monitoring systems for detecting the position of animals in the enclosure. Then, in the practical part, I test three types of cameras for image detection of rat position. Evaluation software for motion analysis will largely be solved in the follow-up diploma thesis.Project made like monitoring and detecting software based on OpenCV.