During the first few hours of recovery from prolonged exercise, co-ingestion of protein (PRO) with carbohydrate (CHO) will increase the rate of muscle glycogen resynthesis if the rate of CHO intake is sub-optimal and/or when the feeding intervals are > 1 h apart (Burke et al.. 2004). It remains controversial whether the higher rate of glycogen resynthesis is attributable to a PRO-mediated increase in insulin release or simply the result of higher energy intake (Jentjens et al., 2001). The optimal rate of CHO ingestion necessary to maximize glycogen resynthesis remains unknown, although some studies have recommended a peak CHO intake of >1.2 g/kg/h. PURPOSE: We examined whether the addition of PRO or “extra CHO” to a drink that provided 1.2 g CHO/kg/h would increase muscle glycogen resynthesis during recovery from prolonged exercise. METHODS: Six men (22± 1 yr; V02|,eilk= 48±8 ml/kg/min) were studied during a 4-h recovery period on 3 separate occasions after a standardized 2-h exercise protocol designed to substantially lower muscle glycogen. Subjects randomly consumed 1 of 3 drinks during recovery from each trial: 1.2 gCHO/kg/h (CHO), 1.6 gCHO/kg/h (CHO/CHO) or 1.2 gCHO + 0.4 gPRO/kg/h (CHO/PRO). Drinks were consumed immediately post-exercise and at 15 min intervals for 3 hrs. RESULTS: Biopsies obtained at 0 and 4 hrs post-exercise revealed no difference in muscle glycogen resynthesis rates between trials (CHO: 22.7±6.6; CHO/CHO: 25.0±3.0; CHO/PRO: 24.6±4.2 mmol/kg dry wt/h) despite differences in energy intake between trials. The ingestion of additional CHO or PRO did not induce changes in blood [insulin ] and blood [glucose] compared to a 1.2 gCHO/kg/h beverage. Muscle [lactate] increased from immediately post-exercise to 4 hrs into recovery in all 3 trials (main effect for time. P<0.05). All subjects reported some degree of gastro-intestinal(Gl) distress after 3 and 4 hrs of recovery but there were no differences between treatments (main effect for time, P<0.05 vs. 0 hrs). CONCLUSION: Ingesting 1.2 gCHO/kg/h met or exceeded the threshold necessary to optimize muscle glycogen synthesis during the first 4 hrs of recovery from prolonged, strenuous exercise in recreationally active men. The ingestion of >1.2 gCHO/kg/h at a concentration of -20% solution induced GI distress in some individuals and may hamper subsequent same-day performance. / Thesis / Master of Science (MS)

xiv, 233 p. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. / In general, postexercise hypotension is characterized by a sustained increase in systemic vascular conductance that is not completely offset by ongoing increases in cardiac output. These hemodynamic changes are present immediately after a single bout of moderate-intensity dynamic exercise in healthy (sedentary and endurance exercise-trained) and hypertensive humans. The mechanisms underlying this postexercise hypotension are currently under investigation; however, the overall hemodynamic response may be altered in response to different factors related to sex, training status, and fluid regulation. The purpose of this dissertation was to investigate the contribution of endogenous hormones associated with the normal menstrual cycle and training status and sex on postexercise hemodynamics and to better understand how fluid replacement and heat-stress affect postexercise hemodynamics in a group of highly trained men. In Chapter IV, the contribution of the menstrual cycle and sex to postexercise hemodynamics was investigated. The results showed that postexercise hemodynamics are largely unaffected by sex and factors associated with the menstrual cycle. In Chapter V, the role of heat-stress and fluid replacement on the postexercise cardiac hemodynamics in a group of endurance exercise-trained men was investigated. These data suggest that fluid replacement and heat-stress mitigate the previously observed fall in cardiac output during exercise recovery in trained men. In Chapter VI, the study investigated the hemodynamic profile in well-hydrated sedentary and trained men and women during recovery from exercise. In contrast to previous research, the results showed a lack of variation in the postexercise hemodynamic response across categories of subjects as there was no evidence of a sex and training interaction. Thus, it appears that factors such as heat-stress and fluid replacement can alter postexercise hemodynamics in trained men; however, factors such as menstrual cycle, sex, and training status do not seem to influence the hemodynamic recovery profile. Yet substantial variation in the postexercise response across individuals remains unexplained. This dissertation contains my previously published and my co-authored material. / Adviser: John R. Halliwill

Acute (~2 min) baroreceptor unloading was reported to modulate metaboreflex control of postexercise cutaneous blood flow, but not sweating. We examined whether sustained changes in baroreceptor loading status during prolonged postexercise recovery can alter the metaboreceptors’ influence on heat loss. Thirteen young males performed a 1-min isometric handgrip exercise (IHG) at 60% maximal voluntary contraction followed by 2-min of forearm ischemia (to activate metaboreceptors) before and 15, 30, 45 and 60-min after a 15-min intense treadmill running exercise (>90% maximal heart rate) in the heat (35°C). This procedure was repeated on three separate days with the application of lower-body positive (LBPP, +40 mmHg), negative (LBNP, -20 mmHg), or no pressure (Control) postexercise. Sweat rate (ventilated capsule; forearm, chest, upper back) and cutaneous vascular conductance (CVC; forearm, upper back) were measured. Relative to pre-IHG levels, sweating at all sites increased during IHG and remained elevated during ischemia at baseline and similarly at 30, 45, and 60-min postexercise (site average sweat rate increase during ischemia: Control, 0.13±0.02; LBPP, 0.12±0.02; LBNP, 0.15±0.02 mg·min-1·cm-2; all P<0.01), but not at 15-min (all P>0.10). LBPP and LBNP application did not modulate the pattern of sweating to IHG and ischemia (all P>0.05). At 15-min postexercise, forearm CVC was reduced from pre-IHG levels during both IHG and ischemia under LBNP only (ischemia: 3.9±0.8 %CVCmax; P<0.02). Therefore, we show metaboreceptors modulate postexercise sweating in the mid-to-late stages (30-60 min) of recovery, independent of baroreceptor loading status and similarly between skin sites. In contrast, metaboreflex modulation of forearm but not upper back CVC occurs only in the early stages of recovery (15 min) and depends upon baroreceptor unloading.

xvii, 168 p. : ill. A print copy of this thesis is available through the UO Libraries. Search the library catalog for the location and call number. / An acute bout of moderate-intensity dynamic exercise results in a sustained rise in skeletal muscle blood flow from that of pre-exercise levels. This postexercise skeletal muscle hyperemia is mediated by two histamine receptors (subtypes, H 1 and H 2 ). Skeletal muscle glucose uptake is also enhanced, in an insulin-independent manner, following moderate-intensity dynamic exercise. The impact of skeletal muscle hyperemia on glucose regulation following exercise has yet to be examined. Therefore, the purpose of this dissertation was to determine if postexercise skeletal muscle hyperemia plays a substantial role in glucose regulation in humans. In Chapter III I tested my ability to block local H 1 - and H 2 -receptors located in the vastus lateralis muscle in humans. The results demonstrate that I was able to successfully block the increase in local blood flow evoked by compound 48-80 with the combination of the H 1 -receptor antagonist pyrilamine and the H 2 -receptor antagonist cimetidine, administered via skeletal muscle microdialysis. In Chapter IV I sought to determine the effect of local combined H 1 - and H 2 -receptor blockade, administered via skeletal muscle microdialysis, on postexercise interstitial glucose concentrations. My findings indicate postexercise delivery of glucose to the interstitial space of the previously active skeletal muscle is mediated, in part, by local H 1 - and H 2 -receptors. In Chapter V I examined the effect of oral administration of H 1 - and H 2 -receptor antagonists on glucose regulation following a postexercise oral glucose load. The results showed that the glycemic and insulin responses to postexercise oral glucose load were more sustained with H 1 - and H 2 -receptor blockade versus control, suggesting a histaminergic effect on postexercise glucose regulation. / Adviser: John Halliwill

The ability of an individual to withstand elevated head-to-toe gravitoinertial (+Gz) forces is determined by the capacity of their body to maintain sufficient head-level arterial pressure. Recent studies have shown a relationship between resting blood-vessel stiffness and an individual’s +Gz-tolerance, although the mechanisms behind this relationship are unclear. The aim of this project is to determine whether or not +Gz-tolerance is affected by a change inresting vasomotor tone. To evaluate this relationship, seven participants were asked to complete a +Gz-tolerance protocol using a human-use centrifugeon two different occasions. On both visits, gradual onset rate (0.1 G.s−1)and rapid onset rate (3.5 G.s−1) tests were done to evaluate the participants+Gz-tolerance. On one of the two visits, prior to the +Gz-tolerance testing,participants performed a 20-min cycle intervention to induce postexercisehypotension, with the aim of temporarily reducing participants’ resting bloodpressure and vasomotor tone. The cycling intervention was successful atinducing postexercise hypotension, as mean arterial pressure was significantlylower on the cycling visit (P&lt;0.05). +Gz-tolerance was significantly lower(P&lt;0.05) on the cycling visit compared with the non-cycling visit for both theGOR and ROR tests (absolute difference of 0.5 G and 0.25 G, respectively).The effect of the type of test on +Gz-tolerance was not influenced by the effectof the cycling intervention (P&gt;0.05). Being the most documented mechanismlinked to postexercise hypotension, sustained vasodilation was assumed tohave occurred. This would have increased distensibility of the affected vessels,explaining the decrease in +Gz-tolerance. The decrease in +Gz-tolerance wassimilar for both tests, indicating that the baroreflex was not affected by thecycling intervention. Assuming that vasodilation occurred, this study showedthat a decrease in resting vasomotor tone decreased +Gz-tolerance, indicatingthe importance of this variable in the relationship between resting blood-vesselstiffness and an individual’s +Gz-tolerance.

Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / A hipotensão pós-exercício (HPE) é um fenômeno de relevância clínica, mas dúvidas persistem no tocante ao efeito do modo e da forma de execução (contínua vs. acumulada) do exercício aeróbio para sua manifestação, bem como o papel do controle autonômico cardíaco como mecanismo fisiológico associado à HPE. Assim, a presente tese objetivou: a) investigar a HPE induzida por sessões aeróbias de exercício isocalórico contínuo e acumulado; b) comparar as respostas de pressão arterial sistólica (PAS) e diastólica (PAD) após teste cardiopulmonar de exercício máximo (TCPE) em três modalidades; c) verificar a influência do modo de exercício e do controle autonômico cardíaco em repouso sobre a reativação vagal após TCPE. No primeiro estudo, 10 homens saudáveis (idade: 27,6 3,5 anos) realizaram TCPEs de corrida e ciclismo para medida do consumo de oxigênio de pico (VO2pico) e sessões contínuas (400 kcal) e acumuladas (2 x 200 kcal) de corrida e ciclismo à 75%VO2reserva. A PAS e PAD reduziram similarmente após exercício contínuo e acumulado (4,6 2,3 vs. 5,2 2,3 mmHg, 2,6 2,5 vs. 3,6 2,5 mmHg, respectivamente, P > 0,05). Porém, a corrida provocou maior declínio na PAS do que o ciclismo (P < 0.05). A atividade simpática (componente de baixa frequência, LF) e parassimpática (componente de alta frequência, HF) aumentou (P < 0,001) e diminuiu (P < 0,001) em relação à sessão controle, elevando o balanço simpato-vagal (razão LF:HF) (P < 0,001) que foi inversamente correlacionado ao &#916;PAS e &#916;PAD (r = -0,41 a -0,70; P < 0.05). No segundo e terceiro estudos, 20 homens saudáveis (idade: 21.2 3.0 anos) realizaram três TCPEs (ciclismo, caminhada e corrida). No segundo estudo, investigou-se a resposta aguda da PA, débito cardíaco (Q), resistência vascular periférica (RVP), sensibilidade do barorreflexo arterial (SBR), variabilidade da frequência cardíaca (VFC) e dispêndio energético durante 60 min após os TCPEs e sessão controle. Comparado ao controle, somente a corrida modalidade envolvendo maior dispêndio energético total (P < 0,001) - foi capaz de reduzir a PAS no pós-exercício (P < 0,001). Mudanças na RVP, SBR, LF, e razão LF:HF foram negativamente correlacionadas às variações na PAS (-0,69 a -0,91; P < 0,001) e PAD (-0,58 a -0,93; P &#8804; 0,002). No terceiro estudo, examinou-se a reativação parassimpática após cada TCPE pela raiz quadrada da média do quadrado das diferenças entre intervalos R-R normais adjacentes em janelas de 30 s (rMSSD30s). Apesar da menor FCpico, VO2pico e dispêndio energético no ciclismo vs. caminhada e corrida (P < 0,001), a reativação parassimpática foi significativamente mais rápida após o ciclismo (P < 0,05). Outrossim, o &#916; rMSSD30-180s foi positivamente correlacionado ao HF (rs = 0,90 a 0,93; P < 0,001) e negativamente correlacionado ao LF e a razão LF:HF medidos no repouso (rs = -0,73 a -0,79 e -0,86 a -0,90, respectivamente; P < 0,001). Em conclusão, a forma de execução do exercício aeróbio não interfere na magnitude da HPE, mas a HPE é dependente do modo ou o volume total de exercício. Os resultados também indicam que o padrão de recuperação do controle autonômico cardíaco pela análise espectral da VFC pode ter um papel importante na indução da HPE. / Postexercise hypotension (PEH) is a phenomenon of clinical relevance, but doubts persist regarding the effect of the mode and manner of execution (continuous vs. cumulative) of aerobic exercise for its manifestation, as well as the role of cardiac autonomic control as physiological mechanisms associated with PEH. Thus, this thesis aimed to: a) investigate the PEH elicited by isocaloric bouts of continuous and accumulative aerobic exercise; b) to compare the acute responses of systolic (SBP) and diastolic blood pressure (DBP) after maximal cardiopulmonary exercise tests (CPET) performed using three exercise modalities; and c) to determine the influence of exercise mode and cardiac autonomic control at rest on the vagal reactivation after CPET. In the first study, ten healthy men (age: 27.6 3.5 yrs) performed maximal CPETs to determine the peak oxygen uptake (VO2peak), and continuous (400 kcal) and accumulated (2 x 200 kcal) exercise bouts of running and cycling at 75% VO2reserve. The SBP and DBP decreased similarly after continuous and accumulated exercise (4.6 2.3 vs. 5.2 2.3 mmHg, 2.6 2.5 vs. 3.6 2.5 mmHg, respectively, P > 0.05). However, running elicited greater SBP reductions than cycling (P < 0.05). The sympathetic (low frequency component, LF) and parasympathetic (high frequency component, HF) activity increased (P < 0.001) and decreased (P < 0.001) from baseline, increasing the sympathovagal balance (LF:HF ratio) (P < 0.001) that was inversely related to &#916;SBP and &#916;DBP (r = -0.41 to -0.70; P < 0.05). In the second and third studies, 20 healthy men (age: 21.2 3.0 yrs) performed three CPETs (cycling, walking and running). The second study investigated the acute response of BP, cardiac output (Q), peripheral vascular resistance (PVR), spontaneous baroreflex sensitivity (SBR), heart rate variability (HRV) and energy expenditure during 60 min after exercise and a control session. Compared to the control, only running the exercise mode involving greater energy expenditure (P <0.001) - was able to reduce the SBP after exercise (P <0.001). Changes in SVR, BRS, LF, and LF:HF ratio were negatively correlated to variations in SBP (range -0.69 to -0.91; P < 0.001) and DBP (range -0.58 to -0.93; P &#8804; 0.002). The third study examined the parasympathetic reactivation after each CPET by the root mean square of successive R-R differences calculated for consecutive 30-s windows (rMSSD30s). Despite lower HRpeak, VO2peak and energy expenditure in cycling vs. walking and running (P < 0.001), parasympathetic reactivation was significantly faster after cycling (P < 0.05). Furthermore, &#916;rMSSD30-180s was positively related to the HF (rs = 0.90 to 0.93; P < 0.001) and negatively related to the LF and LF:HF ratio (rs = -0.73 to -0.79 and -0.86 to -0.90, respectively; P < 0.001) assessed at rest. In conclusion, continuous or accumulated bouts of aerobic exercise do not affect the magnitude of PEH; but the PEH is dependent on exercise mode or the total volume of exercise. The results also indicate that the recovery pattern of HRV may have an important role in eliciting PEH.

Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro / A hipotensão pós-exercício (HPE) é um fenômeno de relevância clínica, mas dúvidas persistem no tocante ao efeito do modo e da forma de execução (contínua vs. acumulada) do exercício aeróbio para sua manifestação, bem como o papel do controle autonômico cardíaco como mecanismo fisiológico associado à HPE. Assim, a presente tese objetivou: a) investigar a HPE induzida por sessões aeróbias de exercício isocalórico contínuo e acumulado; b) comparar as respostas de pressão arterial sistólica (PAS) e diastólica (PAD) após teste cardiopulmonar de exercício máximo (TCPE) em três modalidades; c) verificar a influência do modo de exercício e do controle autonômico cardíaco em repouso sobre a reativação vagal após TCPE. No primeiro estudo, 10 homens saudáveis (idade: 27,6 3,5 anos) realizaram TCPEs de corrida e ciclismo para medida do consumo de oxigênio de pico (VO2pico) e sessões contínuas (400 kcal) e acumuladas (2 x 200 kcal) de corrida e ciclismo à 75%VO2reserva. A PAS e PAD reduziram similarmente após exercício contínuo e acumulado (4,6 2,3 vs. 5,2 2,3 mmHg, 2,6 2,5 vs. 3,6 2,5 mmHg, respectivamente, P > 0,05). Porém, a corrida provocou maior declínio na PAS do que o ciclismo (P < 0.05). A atividade simpática (componente de baixa frequência, LF) e parassimpática (componente de alta frequência, HF) aumentou (P < 0,001) e diminuiu (P < 0,001) em relação à sessão controle, elevando o balanço simpato-vagal (razão LF:HF) (P < 0,001) que foi inversamente correlacionado ao &#916;PAS e &#916;PAD (r = -0,41 a -0,70; P < 0.05). No segundo e terceiro estudos, 20 homens saudáveis (idade: 21.2 3.0 anos) realizaram três TCPEs (ciclismo, caminhada e corrida). No segundo estudo, investigou-se a resposta aguda da PA, débito cardíaco (Q), resistência vascular periférica (RVP), sensibilidade do barorreflexo arterial (SBR), variabilidade da frequência cardíaca (VFC) e dispêndio energético durante 60 min após os TCPEs e sessão controle. Comparado ao controle, somente a corrida modalidade envolvendo maior dispêndio energético total (P < 0,001) - foi capaz de reduzir a PAS no pós-exercício (P < 0,001). Mudanças na RVP, SBR, LF, e razão LF:HF foram negativamente correlacionadas às variações na PAS (-0,69 a -0,91; P < 0,001) e PAD (-0,58 a -0,93; P &#8804; 0,002). No terceiro estudo, examinou-se a reativação parassimpática após cada TCPE pela raiz quadrada da média do quadrado das diferenças entre intervalos R-R normais adjacentes em janelas de 30 s (rMSSD30s). Apesar da menor FCpico, VO2pico e dispêndio energético no ciclismo vs. caminhada e corrida (P < 0,001), a reativação parassimpática foi significativamente mais rápida após o ciclismo (P < 0,05). Outrossim, o &#916; rMSSD30-180s foi positivamente correlacionado ao HF (rs = 0,90 a 0,93; P < 0,001) e negativamente correlacionado ao LF e a razão LF:HF medidos no repouso (rs = -0,73 a -0,79 e -0,86 a -0,90, respectivamente; P < 0,001). Em conclusão, a forma de execução do exercício aeróbio não interfere na magnitude da HPE, mas a HPE é dependente do modo ou o volume total de exercício. Os resultados também indicam que o padrão de recuperação do controle autonômico cardíaco pela análise espectral da VFC pode ter um papel importante na indução da HPE. / Postexercise hypotension (PEH) is a phenomenon of clinical relevance, but doubts persist regarding the effect of the mode and manner of execution (continuous vs. cumulative) of aerobic exercise for its manifestation, as well as the role of cardiac autonomic control as physiological mechanisms associated with PEH. Thus, this thesis aimed to: a) investigate the PEH elicited by isocaloric bouts of continuous and accumulative aerobic exercise; b) to compare the acute responses of systolic (SBP) and diastolic blood pressure (DBP) after maximal cardiopulmonary exercise tests (CPET) performed using three exercise modalities; and c) to determine the influence of exercise mode and cardiac autonomic control at rest on the vagal reactivation after CPET. In the first study, ten healthy men (age: 27.6 3.5 yrs) performed maximal CPETs to determine the peak oxygen uptake (VO2peak), and continuous (400 kcal) and accumulated (2 x 200 kcal) exercise bouts of running and cycling at 75% VO2reserve. The SBP and DBP decreased similarly after continuous and accumulated exercise (4.6 2.3 vs. 5.2 2.3 mmHg, 2.6 2.5 vs. 3.6 2.5 mmHg, respectively, P > 0.05). However, running elicited greater SBP reductions than cycling (P < 0.05). The sympathetic (low frequency component, LF) and parasympathetic (high frequency component, HF) activity increased (P < 0.001) and decreased (P < 0.001) from baseline, increasing the sympathovagal balance (LF:HF ratio) (P < 0.001) that was inversely related to &#916;SBP and &#916;DBP (r = -0.41 to -0.70; P < 0.05). In the second and third studies, 20 healthy men (age: 21.2 3.0 yrs) performed three CPETs (cycling, walking and running). The second study investigated the acute response of BP, cardiac output (Q), peripheral vascular resistance (PVR), spontaneous baroreflex sensitivity (SBR), heart rate variability (HRV) and energy expenditure during 60 min after exercise and a control session. Compared to the control, only running the exercise mode involving greater energy expenditure (P <0.001) - was able to reduce the SBP after exercise (P <0.001). Changes in SVR, BRS, LF, and LF:HF ratio were negatively correlated to variations in SBP (range -0.69 to -0.91; P < 0.001) and DBP (range -0.58 to -0.93; P &#8804; 0.002). The third study examined the parasympathetic reactivation after each CPET by the root mean square of successive R-R differences calculated for consecutive 30-s windows (rMSSD30s). Despite lower HRpeak, VO2peak and energy expenditure in cycling vs. walking and running (P < 0.001), parasympathetic reactivation was significantly faster after cycling (P < 0.05). Furthermore, &#916;rMSSD30-180s was positively related to the HF (rs = 0.90 to 0.93; P < 0.001) and negatively related to the LF and LF:HF ratio (rs = -0.73 to -0.79 and -0.86 to -0.90, respectively; P < 0.001) assessed at rest. In conclusion, continuous or accumulated bouts of aerobic exercise do not affect the magnitude of PEH; but the PEH is dependent on exercise mode or the total volume of exercise. The results also indicate that the recovery pattern of HRV may have an important role in eliciting PEH.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2017-06-22T14:38:54Z No. of bitstreams: 1 marcelledepaularibeiro.pdf: 2385853 bytes, checksum: 5bb0fb2ea70927048b42aca6a6b0a326 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2017-08-07T19:21:29Z (GMT) No. of bitstreams: 1 marcelledepaularibeiro.pdf: 2385853 bytes, checksum: 5bb0fb2ea70927048b42aca6a6b0a326 (MD5) / Made available in DSpace on 2017-08-07T19:21:29Z (GMT). No. of bitstreams: 1 marcelledepaularibeiro.pdf: 2385853 bytes, checksum: 5bb0fb2ea70927048b42aca6a6b0a326 (MD5) Previous issue date: 2014-03-24 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / INTRODUÇÃO: Em indivíduos com amputação traumática de membros inferiores, o aumento do risco de mortalidade por origem cardiovascular pode ser explicado pelos altos níveis de pressão arterial dessa população. Por outro lado, em diversas doenças, o exercício físico vem sendo adotado como conduta não-medicamentosa para o controle da pressão arterial, visto que uma única sessão de exercício físico aeróbio é capaz de reduzir a resistência vascular periférica e promover, consequentemente, queda significativa dos níveis pressóricos, fenômeno denominado hipotensão pós-exercício. No entanto, não é conhecido se amputados traumáticos apresentam, após uma única sessão de exercício físico, hipotensão pósexercício e se a ocorrência da hipotensão pós-exercício é acompanhada de redução da resistência vascular periférica. MATERIAIS E MÉTODOS: Nove indivíduos do sexo masculino com amputação traumática de membros inferiores participaram desse estudo. O protocolo experimental constou de duas sessões conduzidas em ordem aleatória: uma sessão Controle (repouso) e outra de Exercício Físico (cicloergômetro de membros superiores, 30 minutos, intensidade equivalente à frequência cardíaca do primeiro limiar ventilatório, identificado a partir de teste de esforço submáximo). A pressão arterial clínica (método oscilométrico - DIXTAL® 2023), frequência cardíaca (Polar® RS800cx), fluxo sanguíneo do antebraço e resistência vascular do antebraço (pletismografia de oclusão venosa - Hokanson®), foram medidos antes e após a intervenção em cada sessão. Além disso, a pressão arterial e frequência cardíaca ambulatorial de 24 horas foram medidas após as sessões (CardioMapa®). Para a análise estatística, foi adotado como significativo o valor de p<0,05. RESULTADOS: O exercício físico promoveu redução da pressão arterial sistólica, diastólica e média, quando comparado aos valores pré-exercício e sessão Controle. A redução da pressão arterial foi acompanhada de redução significativa da resistência vascular do antebraço, elevação significativa do fluxo sanguíneo do antebraço e da frequência cardíaca, quando comparado aos valores pré-exercício e sessão Controle. Adicionalmente, o exercício físico resultou em redução significativa da média de 24 horas para pressão arterial sistólica, diastólica e média; redução da média de vigília para a pressão arterial diastólica; redução da média do sono para a pressão arterial sistólica, diastólica e média e; manutenção da frequência cardíaca para as médias de 24 horas, vigília e sono. CONCLUSÃO: Indivíduos com amputação traumática de membros inferiores apresentam, após uma única sessão de exercício físico aeróbio, hipotensão pós-exercício clínica e ambulatorial. A hipotensão pós-exercício clínica foi, pelo menos em parte, justificada pela redução da resistência vascular periférica. / INTRODUCTION: In patients with traumatic lower extremity amputations, the elevated cardiovascular mortality risk can be explained by the elevated blood pressure. However, in several diseases, physical exercise has been adopted as a non-pharmacological therapy to reduce blood pressure, since a single session of aerobic exercise can promote significant reduction in blood pressure, phenomenon called postexercise hypotension. Nevertheless, it is unknown if traumatic amputees presents after a single session of physical exercise, postexercise hypotension and if the occurrence of postexercise hypotension is accompanied by a reduction in peripheral vascular resistance. MATERIALS AND METHODS: Nine male subjects with traumatic lower extremity amputation participated in this study. The experimental protocol consisted of two sessions conducted in random order: one Control session (rest) and other of Physical Exercise (upper body cycle ergometer, 30 minutes, intensity equivalent to heart rate of the first ventilatory threshold). The clinic blood pressure (oscillometric method - DIXTAL® 2023), heart rate (Polar ® RS800CX), forearm blood flow and forearm vascular resistance (venous occlusion plethysmography - Hokanson®) , were measured before and after intervention in each session. Also, the ambulatory blood pressure and heart rate were measured for 24 hours after the sessions (CardioMapa®).The p<0,05 value was adopted as significant. RESULTS: Exercise induced a reduction in systolic, diastolic and mean arterial blood pressure values when compared to pre-exercise and Control session. The blood pressure reduction was accompanied by significant reduction of forearm vascular resistance, significant increase of forearm blood flow and heart rate when compared to pre-exercise values and Control session. In addition, exercise resulted in a significant reduction in 24-hour average for systolic, diastolic and mean arterial blood pressure; reduction in awake average for diastolic arterial blood pressure, reduction of asleep average for systolic, diastolic and mean arterial blood pressure and; maintenance of heart rate in 24-hours, awake and asleep average. CONCLUSION: Individuals with traumatic lower extremity amputation presents after a single session of aerobic exercise, clinic and ambulatory postexercise hypotension. Clinic postexercise hypotension was justified by a reduction in peripheral vascular resistance.