The use of a direct method for measuring respiration and transpiration rates may lead to better storage control systems and closer observation of storage losses. A laboratory scale environmental chamber was constructed and provided with a fan and adjustable louver to obtain variable ventilation flow rates. Flow rates were determined using a pressure sensor and fan characteristic curves which were determined experimentally. A regression equation representing the flow rate versus pressure relation was derived and used for estimating the flow rates throughout the study. / Respiration and transpiration were simulated using precision heat sources ranging from 15 to 105W in 15W interval and an evaporative pad. Measured wet and dry bulb temperatures and ventilation flow rates were used for the estimation of heat and moisture generated inside the chamber. The work was performed in two parts. In the first, precision heat sources were used and the heat balance equation components were solved. / In the second part, heat and moisture were generated inside the chamber, heat and moisture balance equations as well as psychrometric equations were solved. (Abstract shortened by UMI.)

Thesis (MScAgric)--Stellenbosch University, 2002. / ENGLISH ABSTRACT: Internal breakdown (internal browning - IB and gel breakdown - GB), over maturity and immaturity are the main factors adversely affecting the quality of exported South African plums. Maturity problems occur when plums are strip harvested, i.e., all the fruit in a block or orchard are harvested once, when the majority of the fruit are at optimum maturity. This results in both ovennature and immature fruit being harvested and contributes to a high percentage of fruit being rejected for export. In general, internal browning occurs when plums are exported under a single 10\,,, temperature regime and gel breakdown occurs when plums are exported under a dual temperature regime. However, GB can also occur at harvest in fruit that are very ripe and may occur at single low temperatures, where it would be masked by lB. While it is known that some cultivars, like 'Angeleno', can withstand a single temperature regime, others cannot. To the best of our knowledge, the reason for this difference is not understood. Many factors affect the quality of plums, including light incidence in the canopy, mineral nutrition and harvest maturity. Plums that were grown on high density training systems such as 'a V- or spindle system yielded consistently high quality fruit with low incidences of gel breakdown. This was in contrast to earlier findings where low-density training systems produced high levels of GB, especially in the lower part of the tree canopy. Branches that were shaded with 80% shade netting yielded fruit with high levels of GB, indicating that the main effect of improved canopy structure on quality was improved light management. A postharvest boron application on 'Songold' plums prior to storage had no effect on the incidence of intemal breakdown in the fruit, but did damage the cuticle, resulting in severe shrivel. However, internal conductivity and firmness measurements indicated that there was some effect of the boron on cell membranes. A more thorough investigation of pre- and postharvest application of boron is recommended in order to determine whether there could be a positive effect of boron in improving fruit quality in plums. Four cultivars of plums ('Pioneer', 'Sapphire', "Songold ' and 'Angeleno') were harvested throughout, and extending beyond, the commercial harvesting period. In all cultivars, the drop in firmness between harvests was not as great as expected and the later harvested fruit were of a similar, if not superior, quality as compared to the earlier harvested fruit. Later harvested plums tended to have higher TSS and better colour development. Contrary to what was expected, later harvested fruit did not have more internal disorders than earlier harvested fruit. This indicates the importance of harvesting at optimum maturity. 'Angeleno' plums had no internal disorders, even after five weeks of cold storage at a single low temperature. 'Pioneer' and 'Sapphire' plums were classified as climacteric and 'Songold' and 'Angeleno' were classified as suppressed climacteric based on ethylene production. The climacteric plums respired and produced ethylene at a higher rate than the suppressed climacteric plums. Climacteric plums ripened faster during shelf life than suppressed climacteric plums. Furthermore, while climacteric plums did not need a cold storage period prior to ripening, suppressed climacteric plums needed a cold storage period in order to ripen normally. The longer the cold storage period prior to transfer to higher temperatures, the faster the plums ripened and the higher the ethylene production at the higher temperature. The suppressed climacteric genotype could possibly be incorporated into plum breeding programs in order to extend the storage period and shelflife of new plum cultivars. The long storage times required to ship plums from South Africa to the export markets has necessitated research on postharvest physiology and quality of this fruit. The use of the climacteric and suppressed climacteric system to classify fruit is expected to assist in understanding the different physiological responses of the cultivars and assist in developing handling protocols. Preharvest factors, particularly light and nutrition, also playa role in postharvest quality. / AFRIKAANSE OPSOMMING: Interne verval (interne verbruining en gelverval), oorrypheid en onryp vrugte, is die hoof faktore wat die uitvoer van Suid Afrikaanse pruime negatief beïnvloed. Rypheidsprobleme ontstaan wanneer pruime gestroop-oes word, met ander woorde, al die vrugte in 'n blok of boord word geoes wanneer die meerderheid vrugte optimum rypheid bereik het. As gevolg hiervan word 'n groot persentasie vrugte vir uitvoer afgekeur, omdat hulle te ryp of nie ryp genoeg is nie. Oor die algemeen vind interne verbruining plaas wanneer vrugte onder 'n enkel lae temperatuur uitgevoer word en gelvervel vind plaas wanneer vrugte onder 'n dubbele temperatuur regime vervoer word. Gel verval kan egter in baie ryp vrugte by oes voorkom en mag by enkel lae temperature voorkom .waar dit deur interne verbruining gemaskeer sal word. Kultivars soos 'Angeleno' kan onder enkel lae temperatuur uitgevoer word sonder interne probleme, terwyl ander pruimkultivars me so uitgevoer kan word nie. So ver ons weet, word die rede hiervoor nie goed verstaan nie. Daar is baie faktore wat die kwaliteit van pruime beïnvloed, onder meer lighuishouding, minerale voeding en die rypheid waarby die pruime geoes word. Pruime wat in hoë-digtheid sisteme soos 'n V- of "spindle" groei het goeie kwaliteit vrugte met 'n lae persentasie gelverval gelewer. Dit is in teenstelling met vroeër bevindinge, waar vrugte van lae digtheid boorde hoë persentasies geverval gelewer het, veral in die onderste gedeeltes van die boom. Takke wat met 80% skadunet bedek is het hoër persentasies gelverval as die kontrole gelewer, wat aandui dat die hoof effek van die verbeterde boom struktuur op kwaliteit, verbeterde lighuishouding was. 'n Na-oes aanwending van boor op 'Songold' pruime het geen effek op die voorkoms van gelverval gehad nie, maar het die kutikula beskadig en tot hoë persentasies verrimpeling gelei. Fermheid en inteme weerstand lesings het egter getoon dat daar wel 'n effek van die boor op die selmembrane en selwande was. 'n Meer omvattende ondersoek van voor- en na-oes aanwending van boor word aanbeveel om vas te stelof daar wel 'n positiewe effek van boor in die verbetering van pruim kwaliteit is. Vier pruim kultivars, ('Pioneer', 'Sapphire', 'Songold' en 'Angeleno'), is gedurende, sowel as later as die kommersiële oesperiode geoes. In al die kultivars was die afname in fermheid kleiner as wat verwag is, en vrugte wat later geoes is het dieselfde, en soms beter, kwaliteit as die vrugte wat vroeër geoes is gehad. Pruime wat later geoes is het beter kleur en gewoonlik hoër suikers gehad. In teenstelling met wat verwag is, het pruime wat later geoes is nie meer interne verval gehad as die pruime wat vroeër geoes is nie. Dit dui die belangrikheid van optimale oesrypheid aan. 'Angeleno' het geen interne verval gehad nie, selfs na vyfweke opberging by -0.5°C. 'Pioneer' en 'Sapphire' pruirne is as klimakteries en 'Songold' en 'Angeleno' as onderdrukte klimakteries geklassifiseer, gebaseer op etileen produksie. Die klimakteriese pruime het teen 'n hoër tempo gerespireer en etileen geproduseer as die onderdrukte klimakteriese pruime. Gedurende raklewe het klimakteriese vrugte vinniger as onderdrukte klimakteriese vrugte ryp geword. Verder, terwyl klimakteriese pruime nie opbgerging by 'n lae temperatuur nodig gehad het nie, het onderdrukte klimakteriese vrugte welopberging by 'n lae temperatuur nodig gehad om normaal ryp te word. Hoe langer die koel opbergingsperiode was, hoe vinniger het die pruime ryp geword by raklewe en hoe hoër was hulle etileen produksie. Die onderdrukte klimakteriese genotipe kan moontlik in teelprogramme geïnkorporeer word om kultivars met verlengde opbergings- en raklewe te teel. Die lang vervoer tye wat benodig word om Suid Afrikaanse pruime by die uitvoer markte te kry het dit nodig gemaak om navorsing oor die na-oes fisiologie en kwaliteit van pruime te doen. Die klassifisering van pruime as klimakteries of onderdrukte klimakteries kan ons in staat stelom die verskillende fisiologiese reaksies van die kultivars te verstaan en om hanterings prosedures te ontwikkel. Voor-oes faktore, veral lighuishouding en mineraalvoecling speelook 'n rol in na-oes kwaliteit van pruime.

No description available.

No description available.

Dissertation (PhD (Agric))--University of Stellenbosch, 2003. / ENGLISH ABSTRACT: Leaf blackening is a particular problem limiting vase life and marketability of Protea cut flowers. This research investigated suppression of Protea leaf blackening with a specific focus on Protea cv. Sylvia (P. eximia x P. susannae) cut flowers. Leaf blackening decreased significantly with decreasing storage temperatures m 'Sylvia' proteas and this was attributed to lower respiration rate and conservation of carbohydrate. Low storage temperatures were beneficial in short term handling procedures encountered during airfreight. However, use of low temperatures alone during the longer sea freight period was unsatisfactory in either maintaining or extending 'Sylvia' protea vase life. Cooling of 'Sylvia' proteas under vacuum significantly suppressed leaf blackening and was of greater benefit than forced air cooling. Although removal of the uppermost leaves delayed leaf blackening in short term storage no significant benefit was found for longer storage periods. Girdling directly beneath the 'Sylvia' protea flowerhead significantly reduced leaf blackening and in combination with low storage temperatures (O°C) enabled a significant extension in both storage and vase life of 'Sylvia' proteas. 'Sylvia' proteas did not exhibit a climacteric respiration peak during 96 h storage at O°C. Exposure to ethylene did not increase Protea leaf blackening or have a detrimental effect on vase life of either proteas or pincushions evaluated. No beneficial response to sucrose supplementation was found in 'Sylvia' proteas. Analysis of the sugar content of both flowerhead and leaves indicated that glucose supplementation might be of benefit and was investigated. Holding solutions of 2.5 % glucose significantly extended vase life due to a significant reduction in leaf blackening. Vase life was terminated due to flowerhead collapse instead of leaf blackening for the first time in 'Sylvia' protea cut flowers. Vase life was significantly extended by 2:3% glucose pulse solutions and leaf blackening significantly suppressed with increasing glucose pulse concentration. Solution uptake was facilitated by use of high intensity PAR lights in the early morning and was attributed to increased stomata opening and a consequent increase in both transpiration and glucose solution uptake. The faster uptake of glucose solutions in shoots harvested in the afternoon was attributed to higher shoot temperatures and consequent transpiration rate to those harvested in the morning. There was a significant reduction in uptake time with increasing pulse temperature, which enabled vacuum cooling to be performed earlier further benefiting storage and vase life extension. Enclosure of 'Sylvia' proteas in polyethylene (PE) lined cartons did suppress leaf blackening in non-pulsed shoots. However, this had no practical significance on useful vase life, which was terminated at this point due to excessive leaf blackening. Water loss appears to have a minimal influence on 'Sylvia' protea leaf blackening. Shading at four and three weeks prior to harvest coincided with a period of significant flowerhead dry mass increase. It is thought that shading at this point, concurrent with an increased carbohydrate demand by the developing flower head resulted in a temporary limitation in carbohydrate supply resulting in the appearance of preharvest leaf blackening. It would appear that proteas do not store large quantities of carbohydrate. Although accentuating winter light conditions by shading did result in a decrease in carbohydrate content the fact that carbohydrate content was already low precluded shading from having a significant impact on postharvest leaf blackening. The finding that glucose was beneficial in extension of both storage and vase life of 'Sylvia' proteas directed research into its use for other Protea and Leucospermum cut flowers. Significant differences in the response to glucose supplementation were found in both Protea and Leucospermum (pincushions). The significant difference in sensitivity to glucose concentration in 'Pink Ice' proteas (phytotoxic at 2:4%) and 'Susara' proteas (no apparent toxicity), in conjunction with a lack of response in 'Cardinal' proteas, a hybrid from the same parents as 'Sylvia' indicates the need to direct future research to individual cultivars. Glucose supplementation had no beneficial effect on vase life of 'Scarlet Ribbon' and 'Tango' pincushions, whilst significantly extending vase life of 'Cordi', 'Gold Dust', 'High Gold' and 'Succession' pincushions. / AFRIKAANSE OPSOMMING: Blaarverswarting is 'n spesifieke probleem wat die vaasleeftyd en die bemarkbaarheid van Protea snyblomme beperk. In hierdie navorsing is ondersoek ingestel na die onderdrukking van Protea blaarverswarting met spesifieke fokus op die snyblomme van die kv. Sylvia (P. eximia x P. susannae). Die voorkoms van blaarverswarting by 'Sylvia' het merkbaar afgeneem tydens die verlaging van bergingstemperature. Hierdie afname is toegeskryf aan 'n laer respirasietempo en die behoud van koolhidrate. Lae bergingstemperature in die korttermyn hantering van die produk tydens lugvrag was voordelig. Die gebruik van lae temperature, slegs tydens die langer verskeepingsperiode, was egter onbevredigend vir vaasleeftyd verlenging en onderhoud van 'Sylvia' protea. Die afkoeling van 'Sylvia' proteas onder vakuum het blaarverswarting in 'n groot mate onderdruk en het beter resultate gelewer as geforseerde lugverkoeling. Alhoewel die verwydering van die heel boonste blare blaarverswarting by korttermynopberging vertraag het, het dit geen merkbare voordele vir langer bergingsperiodes ingehou nie. Ringelering direk onder die blomkop van die 'Sylvia' protea het blaarverswarting aansienlik verminder, en saam met lae bergingstemperature (O°C) het dit 'n merkbare verlenging in beide die bergingstyd en die vaasleeftyd van 'Sylvia' proteas teweeggebring. 'Sylvia' proteas het geen klimakteriese respirasiekruin tydens 'n bergingsperiode van 96 uur teen O°C getoon nie. Blootstelling aan etileen het nie die Protea blaarverswarting laat toeneem of 'n nadelige effek op die vaasleeftyd van die proteas of speldekussings wat geevalueer is, gehad nie. Geen voordelige reaksie op sukrose-byvoeging is in 'Sylvia' proteas gevind nie. 'n Analise van die suikerinhoud van beide die blomkoppe en die blare het aangetoon dat 'n glukose-byvoeging moontlik voordelig kon wees, en hierdie aspek is ondersoek. Met stooroplossings van 2,5 % glukose is die vaasleeftyd aansienlik verleng omdat daar 'n merkbare afname in blaarverswarting was. Vir die eerste keer in die geval van die 'Sylvia' protea, het die vaasleeftyd van die snyblomrne tot 'n einde gekom omdat die blornkoppe uitmekaar gebreek het en nie omdat blaarverswarting ingetree het nie. Die vaasleeftyd is aansienlik verleng met ~ 3% glukose-pulsoplossings, en blaarverswarting is merkbaar onderdruk met die verhoging van hierdie oplossings se glukosekonsentrasie. Die opname van die oplossings is gefasiliteer deur hoe intensiteit PAR (fotosinteties-aktiewe radiasie) ligte vroeg in die oggend, en is toegeskryf daaraan dat meer huidmondjies oopgegaan het. Dit het gelei tot 'n toename in transpirasie en 'n toename in die opname van die glukose-oplossing. Die feit dat glukose-oplossings vinniger opgeneem is deur lote wat in die middag geoes is, is toegeskryf daaraan dat loottemperature dan hoer is as soggens en gevolglik lei tot 'n vinniger transpirasietempo. Daar was 'n merkbare afname in die opnametyd wanneer die temperatuur van die pulsoplossings verhoog is. Vakuumafkoeling kon dus vroeer toegepas word, wat 'n verlenging in bergingstyd en vaasleeftyd tot gevolg gehad het. Verpakking van 'Sylvia' proteas in kartonne wat met poli-etileen uitgevoer is, het blaarverswarting van lote wat nie aan pulsering onderwerp is nie, onderdruk. Hierdie maatreel het egter geen praktiese waarde met betrekking tot vaasleeftyd nie; die vaasleeftyd het tot 'n einde gekom as gevolg van omvangryke blaarverswarting. Dit lyk asof waterverlies weinig invloed het op die blaarverswarting van' Sylvia' proteas. Die vermoede bestaan dat lae koolhidraatvlakke proteas ontvanklik maak vir blaarverswarting. Alhoewel die beklemtoning van winterligtoestande deur beskaduwing gelei het tot 'n afname in koolhidraatinhoud, was hierdie inhoud reeds laag en blaarverswarting na die oes is nie beinvloed nie. Beskaduwing tydens die vier en drie weke voor oestyd het saamgeval met 'n tydperk van aansienlike toename in die droe massa van die blomkop. Die vermoede bestaan dat beskaduwing tydens hierdie fase, saam met die toename in die ontwikkelende blomkop se behoefte aan koolhidrate, aanleiding gegee het tot 'n tydelike beperking in koolhidraatvoorraad wat die voorkoms van blaarverswarting voor die oes tot gevolg gehad het. Die bevinding dat glukose voordelig is vir die verlenging van beide die bergingstyd en die vaasleeftyd van 'Sylvia' proteas het die navorsing gerig om ook ondersoek in te stel na die gebruik daarvan vir ander Protea en Leucospermum snyblomme. Merkbare veranderinge is gevind in die reaksie op glukosebyvoegings in beide Protea en Leucospermum (speldekussings). Die opmerklike verskil in sensitiwiteit vir glukosekonsentrasie in 'Pink Ice' proteas (fitotoksies by ~ 4%) en 'Susara' proteas (geen klaarblyklike toksisiteit), saam met 'n gebrek aan reaksie by 'Cardinal' proteas, 'n hibried van dieselfde ouers as 'Sylvia', dui aan dat verdere navorsing op individuele kultivars toegespits sal rnoet word. Glukosebyvoegings het geen voordelige uitwerking op die vaasleeftyd van 'Scarlet Ribbon' en 'Tango' speldekussings gehad nie, terwyl dit die vaasleeftyd van 'Cordi', 'Gold Dust', 'High Gold' en 'Succession' speldekussingkultivars merkbaar verIeng het.

Post-harvest cane deterioration in the South African sugar industry results in significant revenue loss that is estimated to be in the region of ZAR 60 million per annum. Despite these large losses, precise biological data relating to the process of cane deterioration under South African conditions is limited. Severity of deterioration is influenced by a number of factors, including the length of the harvest-to-crush delay (HTCD), ambient temperature and harvesting practices. For example, burning of cane prior to harvest may result in rind splitting, which provides entry for microbes, particularly Leuconostoc mesenteroides that may exacerbate deterioration. The effect of these factors on deterioration was examined by quantifying the biochemical and microbiological changes that occur in sugarcane stalks after harvest, with the influence of length of HTCD, degree of L. mesenteroides infection and ambient temperature receiving attention. The primary novelty of the work resides in the analysis of deterioration under tightly regulated temperatures, which were designed to reflect diurnal variations typically experienced during summer and winter in the South African sugar belt. In addition, inoculation of mature internodes with a consistent titre of L. mesenteroides was used as a means to mimic a consistent level of infection of harvested stalks by the bacterium. Metabolites selected for analysis were those both native to the stalk and produced as by-products of microbial metabolism, viz. sucrose, glucose, fructose, ethanol, lactic acid, dextran and mannitol. Simulated HTCDs under summer temperatures resulted in increasing glucose and fructose levels with time, which contrasted to the approximately constant levels of these hexose sugars under winter conditions. Commonly referred to as ‘purity’ in an industrial context, precise determination of the concentration of these hexoses in cane consignments could potentially indicate the extent of deterioration. Despite the detection of a basal concentration of lactic acid in unspoiled cane, the observed increase in concentration of this organic acid over the simulated summer HTCD suggests that this metabolite could also potentially serve as an indicator for postharvest deterioration. In contrast, the investigation indicated that ethanol was an unsuitable biochemical marker for deterioration of L. mesenteroides infected cane. An inability to detect dextran and mannitol in the samples, combined with consistent sucrose levels and variable mill room data, suggest that extreme proliferation of L. mesenteroides is facilitated primarily by in-field practices, particularly the manner in which cane is prepared prior to harvest and transport to the mill. Bacterial proliferation and infection by L. mesenteroides of inoculated stalks were monitored by standard selective culturing techniques. Despite the limited detection of L. mesenteroides-associated metabolites, culture-based analyses revealed that the bacterium was the dominant bacterial species within the samples. A number of other bacterial species were isolated and identified, however the extent to which the total number of microorganisms proliferated was limited to a maximum of 1 x 105 colony forming units per gram of fresh tissue. In conjunction with these analyses, a molecular approach known as Polymerase Chain Reaction-Mediated Denaturing Gradient Gel Electrophoresis (PCR-DGGE) was undertaken to investigate the bacterial diversity patterns associated with deteriorating sugarcane stalks throughout the delay period. In contrast to the results obtained by means of the culture-based assays, PCR-DGGE revealed that L. mesenteroides was not the dominant bacterial population, and showed that the level of bacterial diversity was relatively consistent across the differing treatments and with time. The use of complimentary culture-dependent and cultureindependent analyses thus permitted the detection of this discrepancy and indicated the utility of PCR-DGGE in the determination of bacterial community structure of postharvest sugarcane tissue. The biology of post-harvest deterioration of green sugarcane stalks is highly complex, even under rigorously controlled temperature and infection regimens. The results of this study emphasize the important effects that harvest method and environmental conditions have on post-harvest sugarcane deterioration. Towards the formulation of industry-relevant recommendations for combating post-harvest deterioration, future work will strive to mimic the effects that harsh harvesting and transport practices have on the severity of the problem. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

Thesis (MScAgric)--University of Stellenbosch, 2005. / ENGLISH ABSTRACT: Shrivelling and friction discolouration (FD), as postharvest disorders, negatively influence the marketability and potential shelf life of pears. By investigating the contributing factors in each of the disorders, the potential involvement of handling and storage variables were determined. This allowed for a better understanding of the responsible factors that create susceptible environments for these disorders to occur. From the moment that pears are harvested they lose weight by means of transpiration and, to a lesser extent, respiration. When excessive losses are experienced, the fruit will appear shrivelled and the marketability and shelf life are negatively influenced. By minimizing the rates of weight loss, the occurrence of shrivelling among pears during the postharvest handling can be lessened. The periods that proved to be most conducive to shrivelling (during a simulated postharvest handling duration) were where temperatures above 0 °C were experienced. These short periods proved more perilous for shrivelling than lengthy storage durations at low temperatures. This influenced the transpiration rate in such a way that the driving force accelerated the rate of weight loss in all the cultivars that were studied. Removing field heat from fresh produce and maintaining the cold chain reduces the driving force behind the transpiration of the pears. In all the cultivars studied, ‘Packham’s Triumph’, ‘Beurrè Bosc’ and ‘Forelle’, smaller and less mature fruit were more inclined to appear shrivelled. The surface area to volume ratio is fundamental in determining the rate of weight loss. This was most evident in ‘Beurrè Bosc’. Although no reproducible results could be obtained from the morphological studies, literature has attributed this phenomenon to the composition and quantity of the cuticle layer. Reduction of weight loss was obtained by sealing of the fruit stem. This obstructed water movement from the fruit through the xylem conducting tissue to the surrounding atmosphere. Not only did the stem appear greener and fresher, but less weight loss and subsequent shrivel was noticeable in the treated fruit. This effect was most evident in ‘Packham’s Triumph’ and ‘Beurrè Bosc’, but not in ‘Forelle’. ‘Forelle’ typically has a very short, thin stem in comparison to the other two cultivars.All the cultivars showed visual shrivel symptoms after 11 days at 18 °C. Rate of weight loss was the lowest in ‘Packham’s Triumph’, but due to its prominent dimensions, it appeared shrivelled before any of the other cultivars. ‘Beurrè Bosc’ lost weight at the highest rate (0.42%.day-1). As fruit injury, in the presence of oxygen, is inevitable, the oxidative enzymatic browning of pears will always be troublesome. This defensive mechanism partially prevents the infection of the fruit where epidermal cells are injured. To minimize FD, impact and frictional forces need to be lower during both harvesting and handling practices. A laboratory scale method was developed through which reproduceable treatments could be performed, thereby subjecting the fruit to industry related friction, rather than impact, injury. By assessing the discolouration in terms of both extent and intensity, the influence of variables could be determined on both ‘Packham’s Triumph’ and ‘Doyenne du Comice’ pears. As also found in practice, ‘Doyenne du Comice’ proved to be far more susceptible to FD than ‘Packham’s Triumph’, although the activity of the enzyme, polyphenol oxidase (PPO) was found to be higher in the latter. Although no significant difference was found between the FD encountered at fruit temperature of 3 °C and 15 °C, discolouration was greater at the higher temperature. This might be attributed to a greater degree of water loss, lower cell turgidity or higher enzyme activity. Thus, fruit taken from storage and sorted directly thereafter will exhibit less FD. The contribution of condensation forming on the fruit, acting as lubrication, cannot be ignored. Such fruit, with high turgor pressure, might again be more susceptible to bruising which will only be revealed well after the injury. Since enzymes, which include PPO, catalyse biochemical reactions, the availability of sufficient substrate most probably regulates the extent of this biochemical discolouration. Harvesting at optimum maturity and preventing any unnecessary friction will most definitely reduce the occurrence of FD. The ultimate challenge remains to optimize sorting and packing conditions without compromising on fruit quality. / AFRIKAANSE OPSOMMING: Verrimpeling en friksie verbruining (FV), as na-oes defekte, het ’n negatiewe invloed op die bemarkbaarheid en potensiële raklewe van pere. Hierdie studie het die bydraende faktore vir elkeen van die defekte ondersoek. Daar is gepoog om die effek van verskillende hanteringsen opbergingsmetodes op bogenoemde defekte te bepaal. ‘n Beter begrip is verkry van die oorsaaklike faktore wat bydra tot die ontstaan van die betrokke defekte. Direk na die oes van pere, begin die vrug gewig verloor as gevolg van veral transpirasie. Oormatige verliese sal lei tot ’n vrug wat verrimpeld voorkom, met ‘n negatiewe invloed op die bemarkbaarheid en raklewe daarvan. Die voorkoms van die verrimpeling van pere tydens die na-oes hantering van die vrugte, kan verminder word deur vermindering van die tempo van gewigsverlies. Die periodes waartydens die verrimpeling veral voorgekom het, (soos gevind in ’n gesimuleerde na-oes hanteringsmodel) was wanneer temperature bo 0 °C ondervind is. Sulke kort periodes was meer geneig om aanleiding te gee tot verrimpeling as die verlengde periodes van opberging by lae temperature. Sulke periodes van hoër temperature het gelei tot versnelde transpirasie en ’n versnelde tempo van gewigsverlies in al die kultivars wat ondersoek is. Die transpirasietempo van pere kan verlaag word deur die verwydering van veld-hitte en deur die streng behoud van die koue-ketting. In al die kultivars wat ondersoek is, ‘Packham’s Triumph’, ‘Beurrè Bosc’ en ‘Forelle’, is gevind dat die kleiner en minder volwasse vrugte meer geneig was tot verrimpeling. Die oppervlak area tot volume verhouding is krities in die bepaling van die tempo van gewigsverlies. Hierdie bevinding was die prominentste in ‘Beurrè Bosc’. Alhoewel geen beduidende resultate verkry kon word van die morfologiese studies nie, is daar verskeie verwysings in die literatuur wat hierdie verskynsel toeskryf aan die samestelling en hoeveelheid van die kutikula laag. Vermindering van gewigsverlies is verkry deur verseëling van die vrugtestingel. Hierdie tegniek het gelei tot ’n blokkering van die watervloei van die vrug na die omgewing deur die xileem weefsel. Verseëling van die stingel het dit groener en varser laat voorkom, en het ook ’n merkbare vermindering in gewigsverlies en die daaropvolgende verrimpeling tot gevolg gehad. Die effek van stingel-verseëling was die prominentste in ‘Packham’s Triumph’ en‘Beurrè Bosc’. Dit was minder duidelik in ‘Forelle’ wat tipies gekenmerk word deur ‘n baie korter, dun stingel in vergelyking met die ander twee kultivars. Al die kultivars het makroskopiese verrimpeling getoon na ‘n opbergingperiode van 11 dae by 18 °C. Die tempo van gewigsverlies was die laagste in ‘Packham’s Triumph’ alhoewel dit eerste verrimpeld voorgekom het. Dit kan toegeskryf word aan die spesifieke afmetings van hierdie betrokke kultivar. ‘Beurrè Bosc’ het vinnigste gewig verloor (0.42%.dag-1). Aangesien die besering van vrugte, in die aanwesigheid van suurstof, onvermydelik is, sal oksidatiewe, ensiematiese verbruining van pere altyd problematies wees. Hierdie verdedigingsmeganisme voorkom tot ’n mate die infeksie van die vrug wanneer epidermale selle beskadig word. FV kan beperk word deur die vrugte tydens oes en hanteringsprosedures so min as moontlik bloot te stel aan impak en friksie kragte. ’n Laboratorium-model is ontwikkel ter nabootsing van die omstandighede in die industrie. Die vrugte is aan friksie, eerder as impak, onderwerp, soos ondervind in die industrie. Die omvang asook die intensiteit van die verbruining is gemeet in beide ‘Packham’s Triumph’ en ‘Doyenne du Comice’ pere. Op hierdie wyse kon die invloed van die onderskeie veranderlikes in elke kultivar bepaal word. Alhoewel die ensiematiese aktiwiteit van die polifenol oksidase ensiem (PFO) die hoogste in ‘Packham’s Triumph’ was, is gevind dat ‘Doyenne du Comice’ veel meer geneig was tot FV as ‘Packham’s Triumph’. Hierdie bevinding bevestig die verskynsel soos in die praktyk gevind. Alhoewel geen betekenisvolle verskil gevind is tussen FV by vrug temperatuur van 3 °C en 15 °C nie, was daar meer verbruining by die hoër temperatuur. Hierdie verskynsel kan toegeskryf word aan ’n groter mate van waterverlies, laer sel turgiditeit en hoër ensiem aktiwiteit. Dus sal vrugte wat direk na opberging gesorteer word, minder FV toon. Die bydrae van die kondensasie wat op die vrug vorm, en as ’n smeermiddel dien, kan nie geïgnoreer word nie. Sulke vrugte met hoër turgiditeit, mag egter meer vatbaar wees vir kneusing, wat egter eers ’n geruime tyd na die kneusing tevoorverskyn mag kom. Aangesien ensieme, wat PFO insluit, as katalis dien in biochemiese reaksies, sal die beskikbaarheid van voldoende substraat, na alle waarskynlikheid die omvang van die biochemiese verkleuring reguleer.Die oes van pere tydens optimum volwassenheid, en die voorkoming van onnodige friksie sal definitief die voorkoms van FV verminder. Die uitdaging is steeds om sortering- en verpakkingstegnieke verder te verfyn sonder om ’n negatiewe invloed op vrugtekwaliteit te hê.

Thesis (MSc (Horticulture))--University of Stellenbosch, 2006. / Rind breakdown of ‘Nules Clementine’ mandarin (Citrus reticulata Blanco) is a physiological rind disorder that develops during storage. The disorder appears following leakage of essential oil from oil glands in the flavedo, which then leaks into and oxidises the albedo. Oxidised tissue appears as brown spots in the rind. Occurrence of this disorder over the years has caused high financial losses to ‘Clementine’ mandarin producers and exporting companies. Therefore, research aimed at solving this problem was identified as a priority by the citrus industry. Several factors have been reported to be associated with rind breakdown of ‘Clementine’ mandarin fruit, and include environmental factors, fruit maturity at harvest, ethylene gas degreening, storage temperature and storage duration, canopy position where fruit are borne, plant growth regulators, and differences in susceptibility among selections. Practical information has been generated on rind breakdown, but the basic physiology of the disorder is still unresolved. The objective of this study was, therefore, to quantify the effects of various factors on the development of rind breakdown of ‘Nules Clementine’ mandarin fruit, as well as to establish an association between rind pigments and rind antioxidant capacity on the development of this disorder. In this study a series of five experiments was conducted, and included quantifying the differences in susceptibility to rind breakdown between ‘Nules’ and ‘Oroval Clementine’ mandarin fruit, investigating the effects of fruit canopy position, harvest date, ethylene gas degreening, storage temperature and duration on the development of rind breakdown. The effect of these factors on rind pigments and antioxidant capacity was also reported. Generally, ‘Nules’ and ‘Oroval Clementine’ mandarin fruit exhibited similar characteristics at harvest, in terms of maturity and antioxidant capacity. After storage, ‘Nules Clementine’ mandarin developed higher levels of rind breakdown than ‘Oroval Clementine’ mandarin. However, the difference in susceptibility to rind breakdown of ‘Nules’ and ‘Oroval Clementine’ mandarin fruit could not be associated with the antioxidant capacity measured at harvest...

Thesis (MSc)--Stellenbosch University, 2002. / ENGLISH ABSTRACT: Successful export of South African fresh fruit requires fruit of a high quality. Variable fruit quality within a consignment is detrimental to effective marketing of the product. The light microclimate under which the fruit develops is one of the factors that affect within-tree and between-tree variation in quality, maturity and yield. Light exposure effects on fruit quality at harvest and after commercial storage and ripening periods, as well as the physiological mechanisms of these responses to light exposure were studied. Increased exposure to light resulted in the development of typical sun leaf characteristics, with the associated increase in leaf nitrogen concentration and photosynthetic rates. Size and mass of 'Laetitia' plums and 'Cripps' Pink' apples increased with increasing exposure to light. Shade treatments were only started after the initial phase of cell / division was complete. Increased size of the fruit was likely due to the improved carbon balance of the exposed foliage and fruit from the end of cell division until harvest. The transpiration stream was higher in the more exposed foliage compared to the shaded parts of the canopy. This was supported by increased transpiration rates and decreased midday water potentials of exposed leaves. 'Songold' plums and 'Rosemarie' pears were also investigated in the first season, but results were not conclusive. Increased exposure to light was associated with advanced maturity of 'Laetitia' plums at harvest. Shaded fruit were able to attain a similar level of maturity as exposed fruit during storage and ripening periods. At harvest and after the storage and ripening periods, exposed fruit had a higher total soluble solid (TSS) content and therefore an improved eating quality. At harvest, blush colour of 'Laetitia' plums increased with increased exposure to irradiance. Blush colour continued to develop during storage and ripening, and after the ripening period it was evident that blush colour development was associated with a dosage effect i.e. exposure to a cumulative level of irradiance gives the fruit the potential to develop a certain amount of blush colour. Fruit exposed to more than 70% photosynthetic photon flux density (PPFD) were .able to develop to a similar level of blush colour, whereas, fruit exposed to less than 50% PPFD were not able to attain the same level of blush colour. Increased exposure to light did not result in advanced maturity of 'Cripps' Pink' apples at harvest, but it did lead to improved blush colour and increased TSS levels. Blush colour of 'Rosemarie' pears was also dependent on exposure to light from four weeks before harvest. Exposed 'Laetitia' plums had a greater whole fruit content of Mn and B, but concentration on a dry mass basis of P, K, and B decreased with increasing light. Exposed 'Cripps' Pink' apples had increased whole fruit content of P, K, Ca, Mg, Mn, Fe, Cu and B, but concentration on a dry mass basis of K and Na decreased with increasing light. Nutrient content is often associated with the incidence of internal disorders of fruit after storage and further investigation of this effect is necessary as internal disorders were virtually absent in this study. The termination of irrigation shortly before harvest in order to advance the maturity of all the fruit to a similar level, and the subsequent strip harvest of the fruit on a single harvest date, is a practice commonly used by South African plum producers to reduce cost and ostensibly to improve fruit quality. The effect of this practice on 'Songold' plum quality at harvest, after storage and after ripening was also studied. Drip-irrigated plums and plums subjected to soil drying had a better eating quality and were more marketable than micro-irrigated and non-droughted fruit. Following commercial storage and ripening periods these fruit were firmer, had a higher TSS content and were of a similar size and mass to micro-irrigated and non-droughted fruit. The extended harvesting period, in contrast to a strip harvest, allowed the fruit that were smaller and less mature at the beginning of the period to attain a greater size and advanced maturity toward the end of the harvesting period. / AFRIKAANSE OPSOMMING: Suksesvolle uitvoer van Suid-Afrikaanse vars vrugte vereis volgehoue hoë gehalte. Wisselvallige vruggehalte binne 'n besending is nadelig vir die effektiewe bemarking van die produk. Die lig mikroklimaat waaronder die vrug ontwikkel is een van die faktore wat variasie in gehalte, rypheidsstadium en opbrengs binne die boom en tussen bome beïnvloed. Die effek van verhoogde ligblootstelling op vruggehalte by oes, na kommersiële opberging en na die rypwordingsperiode, sowel as die fisiologiese meganismes van die reaksie van verhoogde ligblootstelling is bestudeer. Verhoogde blootstelling aan lig lei tot die ontwikkeling van tipiese son-blaar karaktertrekke, met die gepaardgaande verhoging in blaar stikstof konsentrasie en fotosintetiese tempo. Grote en massa van 'Laetitia' pruime en 'Cripps' Pink' appels het toegeneem met verhoogde blootstelling aan lig. Skadu behandelings is eers begin na die einde van die periode van selverdeling. Toenemende grootte van die vrugte is as gevolg van die verbeterde koolstofbalans van die blootgestelde blare en vrugte vanaffase II van vruggroei tot oestyd. Die transpirasie stroom is geallokeer na die blootgestelde blare. Dit word ondersteun deur die verhoogde transpirasie tempo en verminderde middag waterpotensiaal van die blootgestelde blare. 'Songold' pruime en 'Rosemarie' pere is ook bestudeer, maar die uitslae is nie so oortuigend nie. Toenemende blootstelling aan lig is geassosieer met gevorderde rypheid van 'Laetitia' by oes. Dit was moontlik vir skadu vrugte om dieselfde rypheidsvlak as blootgestelde vrugte te bereik, tydens die opberging en rypwording periodes. Teen oestyd en na opberging en rypwording, het blootgestelde vrugte 'n hoër suiker inhoud gehad en dus 'n hoër eetgehalte. Teen oestyd, het die bioskleur van die pruime toegeneem met toenemende blootstelling aan lig. Ontwikkeling van bloskleur het aangehou gedurende opberging en rypwording, en na die die rypwordings periode was dit duidelik dat bioskleur ontwikkeling met 'n dosis-effek geassosieer word, m.a.w. blootstelling aan 'n sekere opgestapelde vlak van lig gee die vrug die potensiaal om 'n sekere hoeveelheid bioskleur te ontwikkel. Vrugte wat meer as 70% ligblootstelling gekry het, het dieselfde bioskleur ontwikkel, maar vrugte wat minder as 50% ligblootstelling gekry het, het minder bloskleur ontwikkel. Toenemende blootstelling aan lig het nie gelei tot gevorderde rypheid van 'Cripps' Pink' appels teen oestyd nie, maar dit het wel gelei tot verbeterde bioskleur en verhoogde suiker inhoud. Bloskleur van 'Rosemarie' pere is afhanklik van blootstelling aan lig kort voor oestyd. Blootgestelde 'Laetitia' pruime het 'n verhoogde vrug inhoud van Mn en B met verhoogde ligblootstelling gehad, maar die konsentrasie van P, K en B op 'n droë massa basis het afgeneem met verhoogde ligblootstelling. Blootgestelde 'Cripps' Pink' appels het 'n verhoogde vrug inhoud van P, K, Ca, Mg, Mn, Fe, Cu en B met verhoogde ligblootstelling gehad, maar die konsentrasie van K en Na op 'n droë massa basis het afgeneem met verhoogde ligblootstelling. Voedingstof inhoud is geassosieer met die voorkoms van interne probleme in vrugte na opberging en verdere navorsing oor hierdie effek is nodig. Die terminering van besproeiing kort voor oestyd met die doelom die rypheid van al die vrugte op dieselfde vlak te kry sowel as die gepaardgaande oes van al die vrugte op een dag, is 'n algemene praktyke wat gebruik word deur Suid-Afrikaanse pruim produsente om kostes te beperk en oënskynlik, om vruggehalte te verbeter. Die effek van hierdie praktyke op 'Songold' pruim gehalte teen oestyd, na opberging en na rypwording is ook bestudeer. Drup-besproeide pruime en pruime wat blootgestel is aan grond uitdroging het 'n beter eetgehalte en is meer bemarkbaar as mikro-besproeide en nie-droogte geïnduseerde vrugte. Na kommersiële opberging en rypwording periodes het hierdie vrugte 'n hoër fermheid en suiker inhoud gehad, en 'n gelyksoortige grootte en massa as mikrobesproeide en nie-droogte geïnduseerde vrugte. Die verlengde oesperiode het die kleiner en minder ryp vrugte aan die begin van die periode 'n kans gegee om toe te neem in grootte en 'n gevorderde vlak van rypheid te bereik aan die einde van die oesperiode.

Thesis (MScAgric)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: Protea cut flowers are exported worldwide but the vase life of some species and cultivars is considerably shortened by post-harvest leaf blackening. Research has established carbohydrate depletion to be positively correlated with this disorder. Consequently, a study had been made of the carbohydrate status of various species and cultivars, as well as the effect of supplemental glucose (pre and post-storage) on leaf blackening. Glucose, fructose, sucrose and starch concentrations of various Protea species and cultivars held in water were measured at harvest, and again at leaf blackening initiation. All measured carbohydrates declined significantly in 'Carnival', 'Pink Ice' and 'Sheila'. In 'Cardinal' all carbohydrate concentrations decreased significantly, except the sucrose concentration in the inflorescence. 'Susara' and 'Ivy' had very high initial carbohydrate concentrations in the leaves which decreased significantly. The very high initial carbohydrate concentrations in the inflorescence of 'Ivy' declined significantly. 'Brenda' differed from the other cultivars and species in that glucose concentrations increased over time. Carbohydrate concentrations of most of the tested proteas declined significantly from harvest to the initiation of leaf blackening. This highlighted the dependence of the leaves and inflorescence on the carbohydrate reserves, further substantiating the carbohydrate depletion theory. The inflorescences were characterized by high fructose and glucose concentrations and low sucrose concentrations when compared to the leaves. It was hypothesized that glucose pulsing and cold storage at 1°C for three weeks would significantly reduce leaf blackening. 'Brenda', 'Cardinal', 'Carnival', 'Pink Ice', 'Susara ' and 'Sylvia' had significantly less leaf blackening with glucose treatments of 4 and 10%. Leaf blackening of 'Sheila', P. cynaroides and P. grandiceps was not significantly reduced by glucose pulsing. P. magnifica showed a small, but significant, reduction in leaf blackening in response to the 3, 6 and 9% treatments after 10 days only, but despite this, leaf blackening was unacceptably high. 'Pink Ice' harvested at the soft tip stage had less leaf blackening than those harvested open or closed. Toxicity symptoms on the leaves, and in some instances flowers, were observed at higher glucose concentrations (8 and 10%) onP. grandiceps, P. cynaroides, 'Cardinal' and 'Sheila'. All glucose treatments resulted in toxicity symptoms on P. magnifica. A decrease in nonstructural carbohydrates post-harvest apparently occurs in all proteas but it appears that only members of the Ligulatae respond to glucose. Glucose pulsing followed by cold storage at 1°C for three weeks in combination with post-storage glucose vase solutions, significantly reduced leaf blackening of some Protea cultivars. Glucose (1 and 2%), with hypochlorite, significantly delayed leaf blackening in 'Cardinal' and 'Sylvia' after seven days. Leaf blackening of 'Brenda', 'Carnival', 'Pink Ice' and 'Susara' was not significantly reduced by the glucose vase solutions. Other disinfectants, in combination with the sugar treatments, need to be evaluated since the hypochlorite treatment had a dehydrating effect on all the cultivars and resulted in increased leaf blackening. Carbohydrate supplementation of protea flowers with glucose, pre and post-storage, will help meet the post-harvest carbohydrate requirements of certain Protea cultivars and species to an extent. Glucose treatments must be seen in conjunction with maintaining the cold chain and when combined with cold chain maintenance, can extend the storage and vase life. / AFRIKAANSE OPSOMMING: Protea snyblomme word wêreldwyd uitgevoer alhoewel die vaasleeftyd van sommige spesies en kultivars beduidend verkort word deur na-oes loof verbruining. Navorsing het koolhidraatverbruik positief gekorreleer met hierdie probleem. Gevolglik is 'n studie gemaak van die koolhidraatstatus van verskeie spesies en kultivars asook die effek van addisionele glukose (voor en na opberging) op loofverbruining. Glukose, fruktose, sukrose en stysel konsentrasies van verskeie Protea spesies en kultivars wat in water gehou is, is bepaal met oes en weer met die eerste tekens van loofverbruining. Al die gemete koolhidraatkonsentrasies het beduidend afgeneem in 'Carnival', 'Pink Ice' en 'Sheila'. In 'Cardinal' het al die koolhidraatkonsentrasies beduidend afgeneem, behalwe vir die sukrosekonsentrasie in die blom. 'Susara' en 'Ivy' het baie hoë begin koolhidraatkonsentrasies in die blare wat beduidend afneem. Die baie hoë inisiële koolhidraatkonsentrasies in die blom van 'Ivy' neem beduidend af met tyd. 'Brenda' verskil van die ander kultivars en spesies deurdat die glukosekonsentrasies toeneem met tyd. Koolhidraatkonsentrasies van die meeste getoetste proteas neem beduidend af vanaf oes totdat die eerste tekens van loofverbruining verskyn. Dit het die afhanklikheid van die blare en blom op die koolhidraatreserwes beklemtoon en daardeur verder die koolhidraatteorie ondersteun. Die blomme is gekarakteriseer deur hoë fruktose- en glukosekonsentrasies en lae sukrosekonsentrasies wanneer dit met die blare vergelyk is.Die hipotese is gestel dat die voorsiening van glukose, vir 'n aantal ure, gekombineerd met koue opberging by 1°C vir drie weke loofverbruining beduidend sal verminder. 'Brenda', 'Cardinal', 'Carnival', 'Pink lee', 'Susara' en 'Sylvia' het beduidend minder loofverbruining met glukose behandelings tussen 4 en 10%. Loofverbruining van 'Sheila', P. cynaroides en P. grandiceps is nie beduidend verminder deur glukose behandelings nie. P. magnifica het 'n klein, maar beduidende verlaging in loofverbruining getoon met die 3, 6 en 9% behandelings na 10 dae, maar ten spyte hiervan was loofverbruining onaanvaarbaar hoog. 'Pink lee' is geoes by die sagte punt stadium en het minder loofverbruining gehad as blomme wat oop of toe geoes is. Toksisiteitsimptome op die blare, en in sommige gevalle blomme, is waargeneem met hoër glukose konsentrasies (8 en 10%) op P. grandiceps, P. cynaroides, 'Cardinal' en 'Sheila'. Alle glukosebehandelings het toksisiteitsimptome tot gevolg gehad op P. magnifica. 'n Afname in nie-strukturele koolhidrate na oes kom waarskynlik voor in alle proteas maar dit wil voorkom of slegs lede van die Ligulatae positief reageer op glukose. Glukosebehandeling gevolg deur koue opberging by 1°C vir drie weke in kombinasie met na-stoor glukose vaasoplossings het loofverbruining van sommige Protea kultivars beduidend verminder. Glukose (l en 2%), saam met hipoehloriet, het loofverbruining beduidend verminder in 'Cardinal' en 'Sylvia' na sewe dae. Loofverbruining van 'Brenda', 'Carnival', 'Pink lee' en 'Susara' is nie beduidend verminder deur die glukose vaasoplossings nie. Ander ontsmettingsmiddels in kombinasie met die suikerbehandelings moet geëvalueer word aangesien die hipoehlorietbehandeling 'n dehidrerende effek op al die kultivars gehad het en 'n toename in loofverbruining tot gevolg gehad het. Byvoeging van glukose by proteablomme, voor en na opberging, sal tot 'n mate help om in die na-oes koolhidraatbehoeftes van sekere Protea kultivars en spesies te voorsien. Glukosebehandelings moet saam met die beheer van die koueketting gesien word en wanneer gekombineerd met koueketting beheer kan dit opberg en vaasleeftyd verleng.