Green roofs are an emerging technology promoted primarily for stormwater management but little has been published about their potential for biodiversity performance. This is the first study to explore the potential for creating prairie-like, non-succulent, native plant communities on unirrigated extensive green roofs in the southeastern United States. Ten experimental green roof platforms were used to: 1) identify native species and methods of establishment appropriate for green roof applications in the southeastern United States; 2) examine the effects of introducing natural soil into a commercially available green roof soil media mixture on the survival and establishment of native prairie species; and 3) examine the composition of early successional green roof plant communities. Eleven planted species were successfully established and 46 colonizing species were identified. It was found that the addition of native prairie soil did not significantly affect survival, overall cover, or biodiversity in terms of species richness and evenness.