Identifying mechanisms that determine who lives and dies is the first step in developing successful restoration techniques for rare species and endangered habitats. We studied interactions that affect establishment of native plant forbs of conservation concern at the seedling stage to support the theoretical basis for restoration activities in Pacific Northwest prairies. Specifically, we tested the hypothesis that seedling establishment is controlled by 1) competition with or 2) facilitation by existing vegetation and that the interaction is mediated in part by environmental stress. We direct-seeded or planted vegetative plugs of Lupinus oreganus, Castilleja levisecta, Erigeron decumbens, Iris tenax and Sidalcea malviflora ssp. virgata into 20 plots with a range of community compositions in high-stress upland prairies at each of three sites. We counted seedlings and estimated cover of plant functional groups as well as litter, bare soil and disturbance then used linear regression to test for effects of these factors on seedling establishment. We found evidence of indirect facilitation of grass on seedling establishment in the first year: higher accumulations of leaf litter increased seedling numbers at two sites. In the second year, there was evidence of facilitation by live vegetation and litter on seedlings at one site, but no net effect of either competition or facilitation at the other two sites. Overall, we found more evidence for positive interactions than we did for competition. In particular, litter appeared to have a positive effect on seedling establishment of L. oreganus and S. malviflora ssp. virgata. This is contrary to the common perception that litter inhibits plant establishment but supports the theory that facilitation is more common in high stress sites; practitioners should consider seeding into leaf litter at some sites. To support a robust approach to conservation and reintroduction of species with dormant seed, we characterized dormancy types and developed germination protocols for S. malviflora ssp. virgata and I. tenax. S. malviflora ssp. virgata has physical dormancy and may have physiological dormancy. Scarification followed by four weeks of cold moist stratification was effective in initiating germination. I. tenax has morphophysiological dormancy which is overcome by four weeks of warm moist stratification followed by 6-12 weeks of cold stratification. We also conducted a meta-analysis of experiments that tested pre-sowing seed scarification of L. oreganus and conclude that breaking physical dormancy prior to direct seeding does not support higher establishment relative to unscarified seeds in this species. / Graduation date: 2012

Thesis (M.S.)--Mississippi State University. Department of Animal and Dairy Sciences. / Title from title screen. Includes bibliographical references.

Issues associated with urban development such as the urban heat island effect, loss of habitat, increased areas of impervious surfaces leading to storm water management concerns are well known. Many designers, engineers and policy creators are sensitive to these issues creating positive change by implementing alternatives to traditional development. Although, the concept of green roofs is not new to the prairies, modern development of this technology has not been fully embraced. Surrounded by concerns of efficacy and longevity here in the harsh northern prairie climate, green roof development and implementation has been slow. The objective of this practicum is to determine what green roof system and what vegetation of the short grass / fescue prairie and mix grass prairie would succeed in a green roof setting. Determining the appropriate planting palette and growth medium depth for the Canadian Prairies is essential for the development of the green roof industry locally.

Issues associated with urban development such as the urban heat island effect, loss of habitat, increased areas of impervious surfaces leading to storm water management concerns are well known. Many designers, engineers and policy creators are sensitive to these issues creating positive change by implementing alternatives to traditional development. Although, the concept of green roofs is not new to the prairies, modern development of this technology has not been fully embraced. Surrounded by concerns of efficacy and longevity here in the harsh northern prairie climate, green roof development and implementation has been slow. The objective of this practicum is to determine what green roof system and what vegetation of the short grass / fescue prairie and mix grass prairie would succeed in a green roof setting. Determining the appropriate planting palette and growth medium depth for the Canadian Prairies is essential for the development of the green roof industry locally.

Thesis (M.S.)--Mississippi State University. Department of Wildlife and Fisheries. / Title from title screen. Includes bibliographical references.

Thesis (M.S.)--Baylor University, 2009. / Includes bibliographical references (p. 65-71).

The vast majority of terrestrial plant species live in symbiosis with arbuscular mycorrhizal fungi (AMF). AMF and plants live in complex networks, with roots of individual plants hosting multiple AMF, and single AMF colonizing multiple plants concurrently. Through the exchange of resources, the two partners of this symbiosis can have great effects on each other, effects which can ripple through both communities. What determines the patterns of associations between the partners is still largely unknown. In this dissertation, I examine a variety of factors, and in particular host identity, that could drive the community composition of AMF in roots. I began by surveying the diversity of AMF in roots of 12 plant species at a remnant bunchgrass prairie in Oregon, U.S.A. (Chapter 2). To do that, I first designed new primers for use in polymerase chain reaction (PCR) to specifically amplify DNA from all Glomeromycota species. Using those primers, I found 36 distinct AMF phylogenetic groups, or operational taxonomic units (OTUs) in the roots from the prairie. The proportion of OTUs in the basal order Archaeosporales was greater than in many other environmental surveys. I also conducted an in silico analysis to predict how effectively previously published primers would detect the whole diversity of OTUs I detected. I then assayed AMF community composition in the roots of 50 plants from nine plant species (Chapter 3). To do that, I designed primers specific to 18 of the OTUs detected in the initial field survey and used them to test for the presence of each OTU in the roots individual plants. I used that data to test if AMF community composition in individual roots correlated with host identity, spatial distribution, or soil characteristics. I found host identity was associated with both the richness and the structure of root AMF communities, while spatial distribution and soil characteristics were not. Finally, I performed an experimental test of the effect of host identity and community context on AMF community assembly (Chapter 4). I grew plants from four native perennial plant species, including two common and two federally endangered plants, either individually or in a community of four plants (with one plant of each species). I analyzed the AMF community composition in the roots of all plants after 12 weeks of growth with exposure to a uniform mix of field soil as inoculum. I found that host species identity affected root AMF richness and community composition, and community context affected AMF richness. Only one of the endangered species was highly colonized by AMF, and I did not detect unique AMF communities associated with it. This dissertation provides information on the diversity of AMF at a remnant bunchgrass prairie, an ecosystem which has been the subject of very few studies of AMF. Although a complex mix of factors interact to determine AMF community composition in roots, this work provides strong evidence that host identity plays a major role in that process. / Graduation date: 2013