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<p>Ovarian stimulation in assisted reproduction often leads to the production of a high number of oocytes. After fertilization of these oocytes, the resulting embryos can be cryopreserved for later use. Vitrification is a recently introduced method for cryostoring embryos, showing high survival rates for both cleavage stage embryos and blastocysts. Characteristic of vitrification are high concentrations of cryoprotectants and ultra fast freezing which makes the material glassily. A major concern with vitrification has been the direct contact of the cryo-solutions with liquid nitrogen. Therefore, sealed containers have been developed and one of these is the Rapid-i™ made by Vitrolife Sweden AB.</p><p>We evaluated this new device using embryos not suitable for embryo transfer or cryopreservation for clinical purposes. Embryos at cleavage stages were first vitrified and then warmed. Outcome parameters were cryosurvival and development to the blastocyst stage. Blastocysts were randomised between the established VitroLOOP™ and the Rapid-i™ as carriers. Outcome parameters were cryosurvival and further development. Our results show that Rapid-i™ gives good survival rates in vitrification for cleavage stage embryos and blastocysts.</p>

With the increasing use of geometry scanners to create 3D models, there is a rising need for fast and robust mesh smoothing to remove inevitable noise in the measurements. While most previous work has favored diffusion-based iterative techniques for feature-preserving smoothing, we propose a radically different approach, based on robust statistics and local first-order predictors of the surface. The robustness of our local estimates allows us to derive a non-iterative feature-preserving filtering technique applicable to arbitrary "triangle soups". We demonstrate its simplicity of implementation and its efficiency, which make it an excellent solution for smoothing large, noisy, and non-manifold meshes. / Singapore-MIT Alliance (SMA)

This essay concerns the site of Pompeii and its preservation. The aim is to investigate what causes the decay of the ruins, how to prevent it and what consequences the tourism has on the site. The material consists of interviews with three Swedish scientists who are currently working with documenting a block in Pompeii. The result reveals a large number of threats against the city, such as climate, tourism and an inadequate economy situation. Examples of solutions are more investments which can be used for restorations and reconstructions, and guards to prevent vandalism. Another alternative is to stop the preservation work and let the ruins decay.

Freezing is one of the important technologies for preservation of foods. In this project, using surimi as a food model, thermophysical properties of frozen foods were evaluated and the freezing process was simulated using a finite element package. To measure temperature-dependent thermal conductivity, a line-source probe system was used. Effects of test conditions and sample history were investigated. Thermal conductivity of Alaska pollock (Theragra chalcogramma) surimi having 0, 4, 6, 8, and 12% cryoprotectant levels was measured in the range of -40 to 30 ° C. Other thermal properties were analyzed using differential scanning calorimetry (DSC) at the same cryoprotectant concentrations and in the same temperature range. Each dynamically corrected DSC thermogram was used to determine initial freezing point, unfreezable water (bound water), apparent specific heat, enthalpy and unfrozen water weight fraction. When water content of the sample is controlled, thermophysical properties of surimi have a relatively weak dependence upon cryoprotectant level in the unfrozen and fully frozen (-40° C) ranges. However, the initial freezing point and the properties just below this point were significantly affected. From measured data, the Schwartzberg thermal property models for frozen foods were investigated. The models agreed well with experimental data. However, possibility for further improvement is demonstrated by using DSC analysis. This research additionally demonstrated the great potential of DSC for measuring and modeling frozen food thermal properties. Using the derived property models, a commercial PC-based finite element package was used to simulate the process of freezing a food block in a plate freezer. The capability of the program to handle temperature-dependent thermal properties and time-dependent boundary conditions enabled a simulation which accounted for measured changes in thermal properties, ambient temperatures and overall heat transfer coefficient. Predicted temperature history agreed well with measured data. Sensitivities of important model parameters, which were varied within their experimental error range, were also investigated using a factorial experimental design method. The result showed that in decreasing order of influencing freezing time prediction, attention should be given to apparent specific heat, block thickness, overall heat transfer coefficient, ambient temperature, thermal conductivity, and density. / Graduation date: 1991

The focus of this thesis is to explore the influence of market power possessed by seed input companies on rent distribution in an identity preserved production and marketing system. This thesis develops a theoretical model to estimate rent distribution between participants in an identity preserved production and marketing system under constrained production and the elicitation of a premium from market development activities in the presence of a range of seed ownership structures. The thesis employs an empirical model to examine rent distribution of two varieties involved in the Canadian Wheat Boards Identity Preserved Contract Program.<p> The theoretical model demonstrates that market development activities for an identity preserved production and marketing program had a diminished impact on farmers when the seed industry possessed a large degree of market power. The finding of the theoretical model were consistent with that of the empirical model, where the price of certified seed for varieties involved in the identity preserved production and marketing program were priced higher than conventional varieties. The difference in price was found to be greater than the premiums offered by the Identity Preserved Contract Program marketing and/or production contracts for Saskatchewan farmers that received average yields and average prices of grain.

Actualistic, sedimentological, and experimental approaches to the study of vertebrate trace taphonomy in the Kenya Rift Valley have permitted the characterization and even quantification of factors that influence trace morphology and preservation potential in semi-arid lake margins. Several important taphonomic factors were identified from the modern lake-marginal sediments of saline, alkaline Lake Bogoria and freshwater Lake Baringo in the Baringo-Bogoria basin (1º N and 36º E). The investigation of these factors, considering vertebrate ecology and sedimentology of the deposits, together with the study of early and later diagenetic processes that cement substrates, provided a framework for the paleoecological interpretation of three Pleistocene fossil footprint localities, also in the Baringo-Bogoria basin. The most important taphonomic factors appear to be related to the semi-arid climate (e.g., high evaporation:precipitation ratio), frequent lake level changes, the closed nature of the lake basins, bedrock geology (mainly volcaniclastic) within the catchment, and the chemical composition of lake and pore waters. Notable factors that cause the alteration, destruction, and stabilization of traces include efflorescent salt crystallization, which may temporarily cement substrates or destroy traces during crystal growth in the capillary fringe; substrate wetting and drying, which can induce soil-crusting and the shrinking and swelling of smectitic clays; and the presence of benthic microbial mats and biofilms, which may temporarily stabilize substrates or contribute to their early cementation by mediating carbonate precipitation. Experiments to quantitatively and statistically test the effects of salt efflorescence, the rate and temperature of substrate drying (e.g., sun-baking), and swelling and non-swelling clays supported field observations. Preservational processes interpreted from Pleistocene footprint-bearing sediments include the early cementation of substrates by carbonates (e.g., calcite), and during prolonged, stable dry phases, the precipitation of zeolitic cements and Mn- and Fe-oxide minerals. ****PLEASE NOTE: This thesis is formatted to be printed double-sided.

Cryopreservation of gametes and embryos is crucial in fertility treatment and fertility preservation. Preservation of oocytes is a more complicated process than preservation of sperm and embryos. According to recent studies, a new preservation technique called vitrification is found to have higher rates of oocytes-survival after warming    The aim of this study was to evaluate VitriBlast™ Kit, for vitrification of oocytes. Vitrification is an ultra-rapid freezing method in the presence with of high concentrations of cryoprotectants which avoids intracellular ice-crystal formation during the freezing and warming process.     In this study, a total number of 117 immature oocytes were used and 62 of these oocytes were vitrified with VitroBlast™ Kit. During this process two different vitrification devices were used, VitroLoop™ and Cryopette®. After warming, the average survival rate for vitrified oocytes was found to be 61% for VitroLoop™ and 15% for Cryopette® (p&lt;0.001). The remaining 55 oocytes were used as a non-frozen control group and the same incubation method as for vitrified oocytes was used. The survival rate for the control groups was higher than for the vitrified groups (93% versus 35%, p&lt;0.001). The results of this study indicate that VitriBlast™ Kit is not suitable for vitrification of oocytes.

Actualistic, sedimentological, and experimental approaches to the study of vertebrate trace taphonomy in the Kenya Rift Valley have permitted the characterization and even quantification of factors that influence trace morphology and preservation potential in semi-arid lake margins. Several important taphonomic factors were identified from the modern lake-marginal sediments of saline, alkaline Lake Bogoria and freshwater Lake Baringo in the Baringo-Bogoria basin (1º N and 36º E). The investigation of these factors, considering vertebrate ecology and sedimentology of the deposits, together with the study of early and later diagenetic processes that cement substrates, provided a framework for the paleoecological interpretation of three Pleistocene fossil footprint localities, also in the Baringo-Bogoria basin. The most important taphonomic factors appear to be related to the semi-arid climate (e.g., high evaporation:precipitation ratio), frequent lake level changes, the closed nature of the lake basins, bedrock geology (mainly volcaniclastic) within the catchment, and the chemical composition of lake and pore waters. Notable factors that cause the alteration, destruction, and stabilization of traces include efflorescent salt crystallization, which may temporarily cement substrates or destroy traces during crystal growth in the capillary fringe; substrate wetting and drying, which can induce soil-crusting and the shrinking and swelling of smectitic clays; and the presence of benthic microbial mats and biofilms, which may temporarily stabilize substrates or contribute to their early cementation by mediating carbonate precipitation. Experiments to quantitatively and statistically test the effects of salt efflorescence, the rate and temperature of substrate drying (e.g., sun-baking), and swelling and non-swelling clays supported field observations. Preservational processes interpreted from Pleistocene footprint-bearing sediments include the early cementation of substrates by carbonates (e.g., calcite), and during prolonged, stable dry phases, the precipitation of zeolitic cements and Mn- and Fe-oxide minerals. ****PLEASE NOTE: This thesis is formatted to be printed double-sided.