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Synthetic Genes for Antimicrobial PeptidesBorrelli, Alexander P 28 April 2003 (has links)
The goal of this project was to clone and express the antimicrobial peptide protegrin 1 (PG-1). Initially a yeast system was chosen but was discarded due to technical difficulties. Invitrogen's bacterial T7 expression system was chosen next to express the peptide. PG-1 expression was verified by anti-his immunoblot and then the peptide was purified by IMAC. Its activity was verified using a Bacillus subtillis radial diffusion assay.
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Synthetic genes for antimicrobial peptidesBorelli, Alexander P. January 2003 (has links)
Thesis (M.S.)--Worcester Polytechnic Institute. / Keywords: antimicrobial peptides; protegrins; synthetic genes. Includes bibliographical references (p. 56-57).
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