• Refine Query
  • Source
  • Publication year
  • to
  • Language
  • 5241
  • 1344
  • 609
  • 380
  • 248
  • 212
  • 212
  • 212
  • 212
  • 212
  • 212
  • 131
  • 90
  • 75
  • 52
  • Tagged with
  • 10142
  • 1585
  • 1143
  • 1059
  • 929
  • 861
  • 855
  • 826
  • 711
  • 686
  • 642
  • 633
  • 603
  • 570
  • 557
  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
111

The design and synthesis of peptide turn mimetics /

Cassidy, Peter Joseph. January 1998 (has links) (PDF)
Thesis (Ph. D.)--University of Queensland, 1999. / Includes bibliographical references.
112

Synthetic and structural studies towards novel backbone peptidomimetics /

Kelso, Michael John. January 2002 (has links) (PDF)
Thesis (Ph. D.)--University of Queensland, 2002. / Includes bibliographical references.
113

Protein chemical synthesis by serine and threonine ligation

Zhang, Yinfeng, 张银凤 January 2014 (has links)
Landmark advances in the field of synthetic protein chemistry have enabled the preparation of complex, homogeneous proteins, including those that carry specific posttranslational modifications (PTMs). In addition, chemical synthesis will allow one to incorporate unnatural elements to generate new biologics with altered properties and functions. Native chemical ligation (NCL) is a milestone in the chemical synthesis of proteins (Kent et al., Science, 1994, 266, 776-779), in which a C-terminal peptide thioester and an N-terminal cysteine (Cys)-containing peptide-both in side-chain unprotected forms-are selectively coupled to generate a natural peptidic linkage at the site of ligation. This method requires a cysteine at the optimal convergent ligation site. However, Cys is one of the least abundant amino acids in natural proteins. Therefore, the development of new ligation methods at other amino acids will be necessary and important in this regard. Along these lines, our laboratory has developed a novel thiol-independent approach-serine/threonine ligation (STL). It uses the N-terminal serine or threonine of a peptide segment to chemoselectively react with another peptide segment with a C-terminal salicylaldehyde ester to form an N,O-benzylidene acetal linked product, followed by acidolysis to afford the final product at the natural Ser/Thr site. To extend the application of STL in chemical protein synthesis, we have developed a robust method for the preparation of peptide salicylaldehyde esters via Fmoc-based solid phase peptide synthesis. Furthermore, we have successfully applied this ligation method in the convergent synthesis of peptide drugs of significant therapeutic importance, including Teriparatide (Forteo), Corticorelin (oCRH), Exenatide (Byetta) and Tesamorelin (hGHRH). Of significance, we have demonstrated the effectiveness of our STL in the assembly of a more complex target of biological interest: human erythrocyte acylphosphatase (~ 11 kDa). In summary, we have developed a new serine/threonine ligation, which can be effectively used to synthesize peptides and proteins. As there are countless serine and threonine residues in natural proteins, particularly those carrying posttranslational modifications, this method is anticipated to offer new opportunities in synthetic protein chemistry and chemical biology. / published_or_final_version / Chemistry / Doctoral / Doctor of Philosophy
114

Structural studies of two nucleoid-associated proteins : histone-like nucleoid-structuring protein H-NS and α-hemolysin expression-modulating protein Hha

Cao, Wei, 曹威 January 2014 (has links)
In prokaryotic cells, the nucleoid contains almost all the genetic materials as well as a number of nucleoid structuring factors. The nucleoid-associated proteins (NAPs) are known to have low molecular weight and the ability to form dimer or oligomer, and most of them can bind to DNA for regulation of gene expression. The Histone-like nucleoid structuring protein H-NS, well studied as one of the NAPs, acts as a global transcriptional repressor. It has independent functional N-terminal domain for oligomerization and C-terminal domain for DNA binding, joined by a flexible linker. H-NS contributes to horizontal genes transfer and responses to environmental factors like temperature or pH, which would influence the oligomerization ability of H-NS and DNA binding. The α-hemolysin expression-modulating protein Hha is a member of the Hha-YmoA family, expressed only in Gram-negative Enterobacteriaceae as a modulator of virulence factors expression. In E. coli, the binding of Hha to H-NS can modulate the expression of α-hemolysin operon, which is essential for the H-NS-regulated gene expression. In this study, both Hha and the oligomerization domain of H-NS (H-NS64) were expressed in E. coli and the purified proteins were crystallized. The Hha crystals diffracted to 2.2 Å; and the HhA/H-NS complex crystals diffracted to 1.8 Å. Both structures were successfully determined by molecular replacement method. Comparisons were carried out between the published apo Hha and H-NS structures and our complex structures. The structures showed the binding details between H-NS and Hha and also conformational changes of each protein, which may indicate how Hha regulates gene expressions through H-NS. / published_or_final_version / Physiology / Master / Master of Philosophy
115

Structural basis for the regulation of GRK2 by G[beta][gamma]

Lodowski, David Thomas 28 August 2008 (has links)
Not available / text
116

The role of protein methylation as a modifier of cellular pathways

Kim, Jeesun 28 August 2008 (has links)
Not available / text
117

Study of sPDZD2 function using in vitro and in vivo approaches

Tam, Siu-man, Tammy., 談少雯. January 2004 (has links)
published_or_final_version / abstract / toc / Biochemistry / Master / Master of Philosophy
118

PROGRESS TOWARD THE CHEMICAL SYNTHESIS OF PROTEINS

Ehler, Kenneth Walter, 1946- January 1972 (has links)
No description available.
119

EVALUATIONS OF PROTEIN QUALITY AND THE RELATIONSHIP OF AMINO ACID BALANCETO APPETITE

Dorflinger, Richard Lawrence, 1943- January 1972 (has links)
No description available.
120

Rhomboid-like proteins and their functions

Luginsland, Christian January 2013 (has links)
No description available.

Page generated in 0.069 seconds